A survey on relationship between body condition score, vaginal pH and some serum biochemical parameters and sex ratio of their offspring in Arabian mares

Document Type : Research Paper

Authors

1 DVM Graduated, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Assistant Professor, Department of Nutrition and Breeding, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

4 Professor, Department of Statistics, Faculty of Mathematics and Computer Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

        Among various animal species, fetal sex determination and the use of methods to make earlier diagnosis of fetus sex possible are of great interest. There are several assumptions to predict the sex ratio of offspring from a given parent, including the relationship between maternal body condition and some factors in the environment. The present study conducted on 25 Arab mares in Ahvaz, Khuzestan, in the southeast of Iran, to investigate the relationship between maternal body condition score (BCS), vaginal pH, blood glucose, triglyceride, cholesterol and urea levels in mares and offspring sex ratio, one month before mating and at mating time, and also the above-mentioned parameters’ changes during these periods. The results showed that the maternal BCS had no significant effect on predicting offspring sex one month before mating, while its effect on offspring sex was significant at mating time. As was expected, with one-unit increase in maternal BCS one month before mating, the proportion of males to females offspring increased by 4.81 times. The U-Mann-Whitney statistical test showed that the mean score of body condition in mares, who had a colt, was significantly better (higher) than in mares with fillies at each time point of the study. However, no significant relationship was found regarding changes in body condition score and offspring gender. It was also found that other variables had no significant effect on offspring sex at any time of evaluation. In general, it can be claimed that BCS assessment can play an effective role in predicting offspring sex ratio.

Keywords


Alexenko, A.P.; Mao, J.; Ellersieck, M.R.; Davis, A.M.; Whyte, J.J.; Rosenfeld, C.S. et al. (2007). The contrasting effects of ad libitum and restricted feeding of a diet very high in saturated fats on sex ratio and metabolic hormones in mice. Biology of Reproduction, 77(4): 599-604.
Allen, C.D.; Robbins, M.N.; Eguchi, T.; Owens, D.W.; Meylan, A.B.; Meylan, P.A. et al. (2015). First assessment of the sex ratio for an east pacific green sea turtle foraging aggregation: Validation and application of a testosterone ELISA. PLos One, 10.10.
Aurich, C. and Schneider, J. (2014). Sex determination in horses—Current status and future perspectives. Animal Reproduction Sience, 146 (1-2): 34-41.
Austad, S.N. and Sunquist, M.E. (1986). Sex-ratio manipulation in the common opossum. Nature, 324 (6092): 58.
Balaci, I.M.; Pàll, E. and Groza, S. (2009). The influence of vaginal pH in mice over the sex of the offspring. Cluj Veterinary Journal, 162: 41-50.
Bermejo-Alvarez, P.; Roberts, R.M. and Rosenfeld, C.S. (2012). Effect of glucose concentration during in vitro culture of mouse embryos on development to blastocyst, success of embryo transfer, and litter sex ratio. Molecular Reproduction and Development, 79(5): 329-336.
Cameron, E.Z. (2004). Facultative adjustment of mammalian sex ratios in support of the Trivers–Willard hypothesis: evidence for a mechanism. Proceedings of the Royal Society of London. Series B: Biological Sciences, 271(1549): 1723-1728.
Cameron, E.Z. and Linklater, W.L. (2000). Individual mares bias investment in sons and daughters in relation to their condition. Animal Behaviour, 60(3): 359-367.
Cameron, E.Z. and Linklater, W.L. (2007). Extreme sex ratio variation in relation to change in condition around conception. Biology Letters, 3(4): 395-397.
Cameron, E.Z.; Linklater, W.L.; Stafford, K.J. and Minot, E.O. (2008). Maternal investment results in better foal condition through increased play behaviour in horses. Animal Behaviour, 76 (5): 1511-1518.
Cameron, E.Z.; Linklater, W.L.; Stafford, K.J. and Veltman, C.J. (1999). Birth sex ratios relate to mare condition at conception in Kaimanawa horses. Behavioral Ecology. 10 (5): 472-475.
Clutton-Brock, T.H. and Iason, G.R. (1986). Sex ratio variation in mammals. The Quarterly Review of Biology, 61(3): 339-374.
Cui, K.H. and Matthews, C.D. (1993). Human X sperms are larger than Y sperms. Nature, 366(6451): 117-118.
Douhard, M. (2017). Offspring sex ratio in mammals and the Trivers and Willard hypothesis: In pursuit of unambiguous evidence. BioEssays, 39(9):17-43.
Goodall, H. and Roberts, A.M. (1976). Differences in motility of human X-and Y-bearing spermatozoa. Reproduction, 48(2): 433-6.
Green, M.P.; Spate, L.D.; Parks, T.E.; Kimura, K.; Murphy, C.N.; Williams, J.E. et al. (2008). Nutritional skewing of conceptus sex in sheep: effects of a maternal diet enriched in rumen-protected polyunsaturated fatty acids (PUFA). Reproductive Biology and Endocrinology, 6(1): 21.
Gutierrez-Adan, A.; Granados, J.; Pintado, B. and De La Fuente, J. (2001). Influence of glucose on the sex ratio of bovine IVM‏/IVF embryos cultured in vitro. Reproduction, Fertility and Development, 13(6): 361-5.
Henneke, D.R.; Potter, G.D. and Kreider, T.L. (1984). Body condition during pregnancy and lactation and reproductive efficiency of mares. Theriogenology, 21(6): 897-909.
Kimura, K.; Spate, L.D.; Green, M.P. and Roberts, R.M. (2005). Effects of D‐glucose concentration, D‐fructose, and inhibitors of enzymes of the pentose pHospHate pathway on the development and sex ratio of bovine blastocysts. Molecular Reproduction and Development, 72(2): 201-7.
Luna-Estrada, A.A.; Vera-Avila, H.R.; Mora, O.; Anguiano-Serrano, B.; Vasquez-Pelaez, C.G. and Shimada, A. (2006). Effect of pre-mating nutritional status in red deer (Cervus elaphus scoticus) hinds on the sex ratio of their offspring. Small Ruminant Research, 65(1-2): 154-60.
Machado, A.F.; Zimmerman, E.F.; HovlandJr, D.N.; Weiss, R. and Collins, M.D. (2001). Diabetic embryopathy in C57BL/6J mice: altered fetal sex ratio and impact of the splotch allele. Diabetes, 50(5): 1193-9.
Mathews, T.J. and Hamilton, B.E. (2005). Trend analysis of the sex ratio at birth in the United States. National Vital Statistics Reports, 53(20): 1-7.
Minkoff, H.O.W.A.R.D.; Grunebaum, A.M.O.S.; Mccormack, W.M. and Schwarz, R.H. (1985). Relationship of vaginitis to the sex of conceptuses. Obstetrics and Gynecology, 66(2): 239-40.
Oyeyipo, I.P.; van der Linde, M. and du Plessis, S.S. (2017). Environmental Exposure of Sperm Sex-Chromosomes: A Gender Selection Technique. Toxicological Research, 33(4): 315-23.
Peippo, J.; Kurkilahti, M. and Bredbacka, P. (2001). Developmental kinetics of in vitro produced bovine embryos: the effect of sex, glucose and exposure to time-lapse environment. Zygote, 9(2): 105-13.
Polák, J.; Mareš, V.; Konrád, R. and Frynta, D. (2015). Offspring sex ratio in domestic goats: Trivers-Willard out of natural selection. Czech Journal of Animal Science, 60(5): 208-15.
Pratt, N.C.; Huck, U.W. and Lisk, R.D. (1987). Offspring sex ratio in hamsters is correlated with vaginal pH at certain times of mating. Behavioral and Neural Biology, 48(2): 310-6.
Roche, J.R.; Lee, J.M. and Berry, D.P. (2006). Pre-conception energy balance and secondary sex ratio—partial support for the Trivers-Willard hypothesis in dairy cows. Journal of Dairy Science, 89(6): 2119-25.
Rosenfeld, C.S. and Roberts, R.M. (2004). Maternal diet and other factors affecting offspring sex ratio: a review. Biology of Reproduction, 71(4): 1063-70.
Sheldon, B.C. and West, S.A. (2004). Maternal dominance, maternal condition, and offspring sex ratio in ungulate mammals. The American Naturalist, 163(1): 40-54.
Silk, J.B. and Strum, S.C. (2010). Maternal condition does not influence birth sex ratios in anubis baboons (Papioanubis). PloSOne, 5(9): 12750.
Whyte, J.J.; Alexenko, A.P.; Davis, A.M.; Ellersieck, M.R.; Fountain, E.D. and Rosenfeld, C.S. (2007). Maternal diet composition alters serum steroid and free fatty acid concentrations and vaginal pH in mice. Journal of Endocrinology, 192(1): 75-81.
Wyman, A.; Pinto, A.B.; Sheridan, R. and Moley, K.H. (2007). One-cell zygote transfer from diabetic to nondiabetic mouse results in congenital malformations and growth retardation in offspring. Endocrinology, 149(2): 466-9.
Zhang, X.; Christenson, L.K. and Nothnick, W.B. (2007). Regulation of MMP‐9 expression and activity in the mouse uterus by estrogen. Molecular Reproduction and Development: Incorporating Gamete Research, 74(3): 321-31.