The Effect of different concentrations of knotgrass )Polygonum avicular( extract on biological parameters of sperm in fowl

Document Type : Research Paper

Authors

1 DVM Graduated Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

2 Associate Professor, Department of Clinical Sciences, Veterinary Medicine Faculty, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

3 Assistance Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran and Reproductive Biotechnology Research Center, ACECR, Tehran, Iran

Abstract

    Antioxidants can disrupt the production of free radicals by neutralizing oxidative stress and increase the proliferation of testicular germ cells and then improve the quality and quantity of sperm fertility indices in breeder chickens. The knotgrass extract (Polygonum avicular) has high levels of phenolic and flavonoid compounds and has high antioxidant properties. This study aimed to investigate the effect of knotgrass extract on the biological parameters of sperm in roster. In this study, hydroalcoholic extracts of knotgrass was prepared in four concentrations of zero, 500, 1000 and 2000 mg/L and added to drinking water for adult Golpayegan official breeders for one week. After one week, the effect of different doses of the extract on the biological parameters of sperm (number, mobility, survival and normal sperm morphology) was evaluated and compared. The Papanicolaou staining was also used to evaluate the sperm abnormalities. The most number of live sperm with the highest motility and normal morphology was observed in group receiving 2000 mg/L of knotgrass extract. Also, application of high dosage of extract significantly reduced the head, middle piece and tail abnormalities of sperm (P≤0.05). The highest number of live sperm with the highest motility and normal morphology was observed in the group receiving 2000 mg/L of knotgrass extract. Also, using high dose of extract significantly reduced head, medulla, and sperm motility abnormalities (P≤0.05). In overall, the use of hydroalcoholic extracts of knotgrass improved some of the biological parameters of sperm. It can be concluded that knotgrass (Polygonum avicular), due to high amount of phenolic and flavonoid antioxidant compounds, improves the sperm quality in fowls.

Keywords

References

Agarwal, A.; Gupta, S. and Sikka, S. (2006). The role of free radicals and antioxidants in reproduction. Current Opinion Obstetrics and Gynecology, 18(3): 325-32.
Ahmadvand, H.; Amiri, H.; Dalvand, H. and Bagheri, S.H. (2013). Various antioxidant properties of essential oil and hydroalcoholic extract of Artemisa persica. Journal of Birjand University of Medical Sciences, 20(2): 68-78.
Almahdi, A.B.; Ondho, Y.S. and Sutopo, A. (2014). Comparative studies of semen quality on different breed of chicken in poultry breeding center temanggung-central java. International Refereed Journal of Engineering and Science, 3(2): 94-103.
Ansari, S.; Brouki Milan, P.; Mohammadnejad, D.; Delazar, A.; Mortazavi, M. and Mohammadi Roushandeh, A. (2014). Effects of Polygonum avicular extract on histological changes of mouse seminiferous tubules after electromagnetic field exposure. Pharmaceutical Sciences, 19(4), 139-144.
Asgari Jahromi, M.; Movahedin, M.; Amanloo, M.; Mowla, G.; Mazaheri, Z. and Batouli, H. (2013). The effects of calligonum extract on sperm parameters and the rate of apoptosis in aged male mice testis tissue. Modares Journal of Medical Sciences: Pathobiology, 16(1): 25-38.
Aziz, N.; Saleh, R.A.; Sharma, R.K.; Lewis-Jones, I.; Esfandiari, N.; Thomas, A.J. and Agarwal, A. (2004). Novel association between sperm reactive oxygen species production, sperm morphological defects, and the sperm deformity index. . Fertility and Sterility, 81(2): 349-54.
Cao, L.; Leers-Sucheta, S. and Azhar, S. (2004). Aging alters the functional expression of enzymatic and nonenzymatic anti-oxidant defense systems in testicular rat in Leydig cells. Journal of Steroid Biochemistry Molecular Biology, 88(1): 61-7.
Dyce, K.M.; Sack, W.O. and Wensing, C.J.G. (2010). Textbook of Veterinary Anatomy. St. Louis, Mo, Saunders/Elsevier. 4th ed., 705-709.
Edirisinghe, W.R.; Murch, A.; Junk, S. and Yovich, J.L. (1997). Cytogenetic abnormalities of unfertilized oocytes generated from in-vitro fertilization and intracytoplasmic sperm injection: a double-blind study. Human Reproductive, 12(12): 2784-91.
Ekrami, H.; Movahedin, M.; Mazaheri, Z. and Mokhtari Dizaji, M. (2014). Evaluation of cryopreserved human spermatozoa parameters after incubating with Iranian traditional herb (Calligonum) extract. Iranian Journal of Reproductive Medicine, 12(6): 89-91.
Hafez, D.A. (2010). Effect of extracts of ginger goots and cinnamon bark on fertility of male diabetic rats. Journal of American Sciences, 6(10): 940-7.
Hsu, C.Y. (2006). Antioxidant activity of extract from Polygonum aviculare L. Biological Research, 39: 281-288.
Iskandar, S.; Mardalestari, R.; Hernawati, R.; Mardiah E. and Wahyu, E. (2006). Pengaruh jenis konsentrasi krioprotektan dan metode thawing terhadap kualitas semen beku ayam Arab. Indonesian  Journal of Animal and Veterinary Science, 11(1): 34-38.
Justesen, U. and Knuthsen, P. (2001). Composition of flavonoids in fresh herbs and calculation of flavonoid intake by use of herbs in traditional Danish dishes. Food Chemistry, 73(2): 245-50.
Khaki, A.; Fathiazad, F.; Nour,i M.; Khaki, A.A.; Maleki, N. and Jabbari Khamnei, H. (2010). Beneficial effects of quercetin on sperm parameters in streptozotocin-induced diabetic male rats. Phytotherapy Research, 24(9): 1285-91.
Lombardo, F.; Sansone, A.; Romanelli, F.; Paoli, D.; Gandini, L. and Lenzi, A. (2011). The role of antioxidant therapy in the treatment of male infertility: An overview. Asian Journal of Andrology, 13 (5): 690-7.
Milan, P.B.; Nejad, D.M.; Ghanbari, A.A.; Rad, J.S.; Nasrabadi, H.T.; Roudkenar, M.H. et al. (2011). Effects of Polygonum aviculare herbal extract on sperm parameters after EMF exposure in mouse. Pakistan Journal of Biological Sciences, 14(13): 720-4.
Rathore, V.S.; Singh, J.P.; Bhardwaj, S.; Nathawat, N.S.; Kumar, M. and Roy, M.M. (2015). Potential of native shrubs Haloxylon salicornicum and Calligonum Polygonoides for restoration of degraded lands in Arid Western Rajasthan, India. Environmental Management, 55(1): 205-16.
Sikka, S.C. (1996). Oxidative stress and role of antioxidants in normal and abnormal sperm function. Frontiers in Bioscience, 1: 78-86.
Zohreh, F.; Nasri, S. and Kerishchi, P. (2015). The effect of quercetin on pituitary–gonadal axis, sperm parameters and testis tissue in male rats. Quarterly Journal of Sabzevar University of Medical Sciences, 22(3): 18-25.

Files

Share

How to cite

Statistics