Histometrical and histophysiological evaluation of female gonad in two fish species yellowfin seabream (Acanthopagrus latus) and sole (Euryglossa orientalis) from the Mussa creek

Document Type : Research Paper

Authors

1 MSc Graduated of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran

2 Associate Professor, Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran

3 Associate Professor, Department of Marine Biology, Faculty of Marine Science, University of Mazandaran, Babolsar, Iran

4 Associate Professor, Department of Fisheries, Faculty of Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran

Abstract

    This study aimed to investigate the gonadal tissue structure and sexual hormones in the yellowfin seabream (Acanthopagrus latus) and sole (Euryglossa orientalis) collected from the Mussa creek. For this purpose, about 1200 fish (10fish/species/station) were collected from five selected stations in Mussa creek including Petrochemical, Gafari, Majidieh, Ghazaleh and Zangi stations  and Genaveh Port station (as control). After the biometrical assay, bleeding was conducted from the fish caudal vine and blood samples were moved to heparinized microtubes and serum was separated after centrifuging. Also, tissue samples were taken from the ovary and fixed in formalin. Tissue samples passed through a routine histological process and the tissue sections were prepared and stained with H&E. later, histological sections were studied under light microscope and microphotographs were prepared using Dino lite with Dinocapture software. The steroid hormones including 17-βestradiol (E2) and testosterone (T) were measured using the radioimmuneassay (RIA) method and gonadotropin hormones (GTHI and GTHII) were also measured by IRMA method. The most follicles observed in the ovarian samples of both species from different stations were in previtellogenic stages including chromatin-nucleolus, prenucleolus and cortical alveoli follicles. Also an increase in the interstitial connective tissue and atretic follicles were recorded in the ovarian samples from some stations especially Petrochemical station. The results showed that the level of steroids (E2 and T) was lower in fish from the Petrochemical station than others, while GTHs reached the highest level in these fish. Generally, the most alterations in gonadal tissue structure in both fish species were recorded in the petrochemical station, probably due to waste waters released into the water in this station from various petrochemical and color alkali industries surrounded this station.

Keywords

References

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