Shahid Chamran University of AhvazIranian Veterinary Journal1735-687313420180121Molecular detection of Theileria equi in dogs from rural areas around AhvazMolecular detection of Theileria equi in dogs from rural areas around Ahvaz585766410.22055/ivj.2017.36795.1613FASomayeh BahramiAssociate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IranAlireza AlborziAssociate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran
University of Ahvaz, Ahvaz, IranBahman MosallanejadProfessor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IranHasan JavanshiriDVM Student, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IranJournal Article20160504 Equine theileriosis is one of the most important parasitic diseases among the equids that poses a serious threat to the horse industry. Proper diagnosis of parasites in carriers is important for prevention and effective control measures. Therefore, the aim of the present study was to investigate the probable presence of <em>Theileria equi</em> infection in dogs from Ahvaz city. A total of 100 blood samples from dogs were examined for the presence of <em>T. equi </em>infection using molecular methods. After DNA extraction from the dog's whole blood, for tracking DNA of <em>T. equi</em> polymerase chain reaction was performed and the results were observed in agarose gel. Out of 100 samples, the PCR method detected <em>T. equi</em> infection in two dogs. In other words, two percent of dogs were probably carriers for <em>T. equi</em>. In conclusion, the present study revealed that dogs from Ahvaz city are infected with <em>T. equi</em>. So these animals can be probably considered as the carriers of <em>T. equi</em>. Therefore, we should pay attention to dog's with <em>T. equi</em> infection in equine theileriosis control programs. Equine theileriosis is one of the most important parasitic diseases among the equids that poses a serious threat to the horse industry. Proper diagnosis of parasites in carriers is important for prevention and effective control measures. Therefore, the aim of the present study was to investigate the probable presence of <em>Theileria equi</em> infection in dogs from Ahvaz city. A total of 100 blood samples from dogs were examined for the presence of <em>T. equi </em>infection using molecular methods. After DNA extraction from the dog's whole blood, for tracking DNA of <em>T. equi</em> polymerase chain reaction was performed and the results were observed in agarose gel. Out of 100 samples, the PCR method detected <em>T. equi</em> infection in two dogs. In other words, two percent of dogs were probably carriers for <em>T. equi</em>. In conclusion, the present study revealed that dogs from Ahvaz city are infected with <em>T. equi</em>. So these animals can be probably considered as the carriers of <em>T. equi</em>. Therefore, we should pay attention to dog's with <em>T. equi</em> infection in equine theileriosis control programs.Shahid Chamran University of AhvazIranian Veterinary Journal1735-687313420180121The inhibitory effect of Lactobacillus rhamnosus LGG 23527on growth and enterotoxin A gene expression of S. aureus ATCC 29213The inhibitory effect of Lactobacillus rhamnosus LGG 23527on growth and enterotoxin A gene expression of S. aureus ATCC 292139185766510.22055/ivj.2017.36885.1615FAMahnoosh ParsaeimehrAssistant Professor, Department Food Hygiene, Faculty of Veterinary Medicine, Semnan University, Semnan,
IranAshakn JabliAssistant Professor, Department Food Hygiene, Faculty of Veterinary Medicine, Semnan University, Semnan,
IranMaryam AzizkhaniAssistant Professor, Department Food Hygiene, Faculty of Veterinary Medicine, Amol University of Special
Modern Technologies, Amol, IranJournal Article20160507 Increase shelf life, food safety and human health improvement by using the natural microflora are the dominant issues in public health. In this research, the effect of <em>Lactobacillus</em><em> rhamnosus</em> (LGG) isolate with intestinal origin was studied on growth and enterotoxin production by <em>Staphylococcus aureus</em> and then, <em>Staphylococcus aureus</em> enterotoxin A gene expression was evaluated in a laboratory media. Therefore, this strain (1*10<sup>7</sup> cfu / ml) and <em>Staphylococcus aureus</em> (1*10<sup>5</sup> cfu / ml) were inoculated in TSB and incubated in two temperatures at 25 and 35°C, at the time of zero, 24, 48 and 72 hours, the mixed cultures were counted in specific media. <em>Staphylococcus</em><em> aureus</em> without lactobacillus was incubated as control and then enterotoxin production evaluated by using ELISA kit and the gene expression of the SEA of <em>S. aureus</em> in mixed and control cultures was also evaluated by RT Real Time PCR technique. The lactobacillus isolates decreased <em>Staphylococcus aureus</em> growth compared to control samples 3 log at 25°C and 2 log at 35°C. The enterotoxin production of A, C and E at 25° and enterotoxin type E and A at 35°C was inhibited by <em>Lactobacillus</em><em> rhamnosus.</em> Comparison of gene expression SEA between treated and control cultures showed the gene expression of the SEA was significantly suppressed 6.82 fold in <em>S. aureus</em> co cultured with studied lactobacillus isolate at 25°C while it was not significant to 35 °C (P<0.05). Because of the reduced effects of the isolates seen in this study, proposed it for natural prevention of bacterial growth/toxin production in foods to improve the quality and safety of the foods. Increase shelf life, food safety and human health improvement by using the natural microflora are the dominant issues in public health. In this research, the effect of <em>Lactobacillus</em><em> rhamnosus</em> (LGG) isolate with intestinal origin was studied on growth and enterotoxin production by <em>Staphylococcus aureus</em> and then, <em>Staphylococcus aureus</em> enterotoxin A gene expression was evaluated in a laboratory media. Therefore, this strain (1*10<sup>7</sup> cfu / ml) and <em>Staphylococcus aureus</em> (1*10<sup>5</sup> cfu / ml) were inoculated in TSB and incubated in two temperatures at 25 and 35°C, at the time of zero, 24, 48 and 72 hours, the mixed cultures were counted in specific media. <em>Staphylococcus</em><em> aureus</em> without lactobacillus was incubated as control and then enterotoxin production evaluated by using ELISA kit and the gene expression of the SEA of <em>S. aureus</em> in mixed and control cultures was also evaluated by RT Real Time PCR technique. The lactobacillus isolates decreased <em>Staphylococcus aureus</em> growth compared to control samples 3 log at 25°C and 2 log at 35°C. The enterotoxin production of A, C and E at 25° and enterotoxin type E and A at 35°C was inhibited by <em>Lactobacillus</em><em> rhamnosus.</em> Comparison of gene expression SEA between treated and control cultures showed the gene expression of the SEA was significantly suppressed 6.82 fold in <em>S. aureus</em> co cultured with studied lactobacillus isolate at 25°C while it was not significant to 35 °C (P<0.05). Because of the reduced effects of the isolates seen in this study, proposed it for natural prevention of bacterial growth/toxin production in foods to improve the quality and safety of the foods.Shahid Chamran University of AhvazIranian Veterinary Journal1735-687313420180121Designing of the expressing eukaryotic plasmid of the G1 epitope of bovine ephemeral fever virus G glycoprotein in human embryonic kidney cellsDesigning of the expressing eukaryotic plasmid of the G1 epitope of bovine ephemeral fever virus G glycoprotein in human embryonic kidney cells19275766610.22055/ivj.2017.60443.1793FAReza PasandidehPhD Student of Animal Genetics and Breeding, Faculty of Animal and Food Technology Ramin Agriculture
and Natural Resources University of Khuzestan, Iran0000-0003-3002-8605Mohamad Taghi Beigi NasiriProfessor, Department of Animal Science, Faculty of Animal and Food Technology Ramin Agriculture
and Natural Resources University of Khuzestan, IranMasoud Reza Seifiabad ShapouriProfessor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of
Ahvaz, Ahvaz, IranJamal FayaziAssociate Professor, Department of Animal Science, Faculty of Animal and Food Technology Ramin
Agriculture and Natural Resources University of Khuzestan, IranHedayatallah RoshanfekrProfessor, Department of Animal Science, Faculty of Animal and Food Technology Ramin Agriculture
and Natural Resources University of Khuzestan, IranMohsen LotfiResearch Assistant Professor, Department of Quality Control of Biological Products, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension, Karaj, IranJournal Article20160830The G glycoprotein of bovine ephemeral fever virus (BEFV) has been identified as a plausible candidate for immunization against BEF disease. In recent years, this protein has been investigated to produce a recombinant vaccine. The aim of the present study was to construct a eukaryotic plasmid, expressing G1 epitope of BEFV G glycoprotein gene, for application as a possible DNA vaccine in future studies. For this purpose, the G1 epitope of G glycoprotein gene was cloned in a eukaryotic expression vector, pEGFP-N1, under the control of the human cytomegalovirus (CMV) promoter. Then, the recombinant pEGFPN1-G1 construct was transfected into human embryonic kidney (HEK 293) cell line and the expression efficiency was verified by indirect immunofluorescent (IFA) staining and immunoblotting techniques. Observation of intracytoplasmic fluorescence in transfected cells and appearance of a distinct band at an approximate molecular weight of 26 kDa in immunoblotting in reaction to an anti-G1 mouse serum, indicated that G1 protein was successfully expressed by this recombinant construct in the host cells.The G glycoprotein of bovine ephemeral fever virus (BEFV) has been identified as a plausible candidate for immunization against BEF disease. In recent years, this protein has been investigated to produce a recombinant vaccine. The aim of the present study was to construct a eukaryotic plasmid, expressing G1 epitope of BEFV G glycoprotein gene, for application as a possible DNA vaccine in future studies. For this purpose, the G1 epitope of G glycoprotein gene was cloned in a eukaryotic expression vector, pEGFP-N1, under the control of the human cytomegalovirus (CMV) promoter. Then, the recombinant pEGFPN1-G1 construct was transfected into human embryonic kidney (HEK 293) cell line and the expression efficiency was verified by indirect immunofluorescent (IFA) staining and immunoblotting techniques. Observation of intracytoplasmic fluorescence in transfected cells and appearance of a distinct band at an approximate molecular weight of 26 kDa in immunoblotting in reaction to an anti-G1 mouse serum, indicated that G1 protein was successfully expressed by this recombinant construct in the host cells.Shahid Chamran University of AhvazIranian Veterinary Journal1735-687313420180121Comparative effects of Aquavac and Iranian streptococcus/lactococcus vaccine on Health Factors in rainbow trout (Onchorhynchus mykiss)Comparative effects of Aquavac and Iranian streptococcus/lactococcus vaccine on Health Factors in rainbow trout (Onchorhynchus mykiss)28425766710.22055/ivj.2017.48182.1696FAHossein KhajPh.D Student of Aquatic Animal Health, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IranMehrzad MesbahProfessor, Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Chamran University
of Ahvaz, Ahvaz, IranMohamad Reza TabandeAssociate Professor, Department of Basic Science, Faculty of Veterinary Shahid Chamran University of
Ahvaz, Ahvaz, IranTakavar MohamadianAssistant Professor, Department of Clinical Science, Faculty of Veterinary Shahid Chamran University of
Ahvaz, Ahvaz, IranMaryam DadarAssistant Professor, Department of Brucellosis, Razi Vaccine and Serum Research Institute, Agricultural
Research, Education and Extension Organization (AREEO), Karaj, IranJournal Article20160406Streptococcosis / Lactococcosis is important diseases for the aquaculture industry special in rainbow trout (Oncorhynchus mykis). The aim of this study was to compare the effective of Streptococcosis divalent foreign and Iranian vaccines (Lactococus garvie and Streptococcus iniae) in the immune responses contains, complement activation, serum lysozyme rate, bactericidal and respiratory burst (NBT) and some hematological indices contains, RBC and WBC count and hematocrit and as well as the growth performance contains, initial weight, final weight, body weight gain percent, specific growth rate, feed conversion ratio, protein efficiency rate, body weight gain percent and condition factor in rainbow trout. In this study, 630 rainbow trout fish weighing about 26 ± 3g were divided to three treatments contains, foreign vaccine, iranian vaccines and control group. During the experimental period and on days 0, 14, 30, 45 and 60 after vaccination samples were taken. The results of this study showed an increase in measured indices of blood and immune responses From 14 to 60 days of experiment in vaccinated trout fish compared to the control group that the amount of foreign vaccine group increased than two other groups. Also the results of this study showed that Iranian vaccine had a better effect on growth performance of rainbow trout Compared to foreign vaccine and control groups. According to the results of this study, vaccination by foreign and Iranian vaccine could have a positive effect on fish health indicators.Streptococcosis / Lactococcosis is important diseases for the aquaculture industry special in rainbow trout (Oncorhynchus mykis). The aim of this study was to compare the effective of Streptococcosis divalent foreign and Iranian vaccines (Lactococus garvie and Streptococcus iniae) in the immune responses contains, complement activation, serum lysozyme rate, bactericidal and respiratory burst (NBT) and some hematological indices contains, RBC and WBC count and hematocrit and as well as the growth performance contains, initial weight, final weight, body weight gain percent, specific growth rate, feed conversion ratio, protein efficiency rate, body weight gain percent and condition factor in rainbow trout. In this study, 630 rainbow trout fish weighing about 26 ± 3g were divided to three treatments contains, foreign vaccine, iranian vaccines and control group. During the experimental period and on days 0, 14, 30, 45 and 60 after vaccination samples were taken. The results of this study showed an increase in measured indices of blood and immune responses From 14 to 60 days of experiment in vaccinated trout fish compared to the control group that the amount of foreign vaccine group increased than two other groups. Also the results of this study showed that Iranian vaccine had a better effect on growth performance of rainbow trout Compared to foreign vaccine and control groups. According to the results of this study, vaccination by foreign and Iranian vaccine could have a positive effect on fish health indicators.Shahid Chamran University of AhvazIranian Veterinary Journal1735-687313420180121A survey on the seroprevalence of Toxoplasmosis in sheep from rural areas of Tabriz by Immunoblotting assaysA survey on the seroprevalence of Toxoplasmosis in sheep from rural areas of Tabriz by Immunoblotting assays43525766910.22055/ivj.2017.64040.1820FAParisa ShahbaziAssistant Professor, Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz,
Tabriz, IranAhmad NematollahiProfessor, Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz,
Tabriz, IranHamed BehniafarDVM Graduated, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, IranAbbas Imani BaranAssistant Professor, Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz,
Tabriz, IranJournal Article20161024Background and purpose: Toxoplasmosis is a zoonotic disease with a worldwide distribution which is caused by Toxoplasma gondii. It can infect various cells of human and approximately all animals. The current study was conducted to determine the seroprevalence of T. gondii in sheep from rural areas of Tabriz by Western blot and Dot blot assays in which T. gondii soluble antigen was reacted with antibodies raised in serum samples of sheep.<br /> Materials and methods: From October 2012 to April 2013, a total of 186 blood samples were collected from sheep (93 ewes and 93 rams) rural areas of Tabriz. Initially, the sera were separated and evaluated to determine the seroprevalence of toxoplasmosis by Dot blot assay. Then, positive serum samples were evaluated by Western blot assay in order to final confirmation of infection and observation of 30 KDa specific bands.<br /> Results: Based on Dot blot assay, the seroprevalence rate in the sheep from rural areas of Tabriz was 9.14%. The seroprevalence of Toxoplasmosis was higher in ewes (11.83%) than that of rams (6.45%). However, this difference was not statistically significant (P> 0.05). The patterns of Western blot assay on all positive sera were manifested as distinct 30 KDa bands.<br /> Conclusion: In this study, no distinct difference was observed on the antigen/protein bands pertaining to infected samples and the proteins with 30 KDa molecular weight and those being smaller than 10 KDa were identified as the immunogenic proteins of T. gondii tachyzoites strain RH.Background and purpose: Toxoplasmosis is a zoonotic disease with a worldwide distribution which is caused by Toxoplasma gondii. It can infect various cells of human and approximately all animals. The current study was conducted to determine the seroprevalence of T. gondii in sheep from rural areas of Tabriz by Western blot and Dot blot assays in which T. gondii soluble antigen was reacted with antibodies raised in serum samples of sheep.<br /> Materials and methods: From October 2012 to April 2013, a total of 186 blood samples were collected from sheep (93 ewes and 93 rams) rural areas of Tabriz. Initially, the sera were separated and evaluated to determine the seroprevalence of toxoplasmosis by Dot blot assay. Then, positive serum samples were evaluated by Western blot assay in order to final confirmation of infection and observation of 30 KDa specific bands.<br /> Results: Based on Dot blot assay, the seroprevalence rate in the sheep from rural areas of Tabriz was 9.14%. The seroprevalence of Toxoplasmosis was higher in ewes (11.83%) than that of rams (6.45%). However, this difference was not statistically significant (P> 0.05). The patterns of Western blot assay on all positive sera were manifested as distinct 30 KDa bands.<br /> Conclusion: In this study, no distinct difference was observed on the antigen/protein bands pertaining to infected samples and the proteins with 30 KDa molecular weight and those being smaller than 10 KDa were identified as the immunogenic proteins of T. gondii tachyzoites strain RH.Shahid Chamran University of AhvazIranian Veterinary Journal1735-687313420180121Effect dietary threonine and tryptophan on performance, blood parameters and mortality in broiler chickens with cold-induced ascitesEffect dietary threonine and tryptophan on performance, blood parameters and mortality in broiler chickens with cold-induced ascites53665767010.22055/ivj.2017.50017.1710FAAbbas AlipanahPhD Graduated of Animal Science, Faculty of Animal Science, Urmia University, Urmia, IranMohsen DaneshyarAssociate Professor, Department of Animal Science, Urmia University, Urmia, IranParviz FarhudmandProfessor, Department of Animal Science, Urmia University, Urmia, IranGholam Reza NajafiAssociate Professor, Department of Basic Sciences, Faculty of Vetrenary Medicine, Urmia University, Urmia,
IranJournal Article20160612BACKGROUND: Ascites is a metabolic disease that causes the economic losses in broiler industry. OBJECTIVES: This study was performed to investigate the effect of threonine and tryptophan on performance, meat quality and quantity, mortality, some enzymes, thyroid hormones, some blood indices and heart parameters of broiler chickens. METHODS: Three hundred one-day-old female chicks (Ross 308) were used in a completely randomized design with four treatments of control (without amino acids) and 140% tryptophan, 130% threonine and combination of both amino acids. Each treatment included the five replicates and 15 birds per each replicate. RESULTS: consumption of tryptophan and threonine caused the improvement of performance, carcass weight and breast meat (P<0.05). The consumption of threonine alone or along with tryptophan decreased the ascites mortality during the whole period (P<0.05). Furthermore, the right ventricle weight to total ventricle ratio was lower in the amino acids (alone or together) fed birds as compared to the birds in control birds (P<0.05). No effects of the amino acids was observed on blood enzymes, thyroid hormones, anus temperature and the blood indices at day 42 of age (P>0.05). CONLUSIONS: taken together, tryptophan and threonine decreases the feed consumption and feed conversion ratio and consequently decrease the ascites mortality in broiler chickens.BACKGROUND: Ascites is a metabolic disease that causes the economic losses in broiler industry. OBJECTIVES: This study was performed to investigate the effect of threonine and tryptophan on performance, meat quality and quantity, mortality, some enzymes, thyroid hormones, some blood indices and heart parameters of broiler chickens. METHODS: Three hundred one-day-old female chicks (Ross 308) were used in a completely randomized design with four treatments of control (without amino acids) and 140% tryptophan, 130% threonine and combination of both amino acids. Each treatment included the five replicates and 15 birds per each replicate. RESULTS: consumption of tryptophan and threonine caused the improvement of performance, carcass weight and breast meat (P<0.05). The consumption of threonine alone or along with tryptophan decreased the ascites mortality during the whole period (P<0.05). Furthermore, the right ventricle weight to total ventricle ratio was lower in the amino acids (alone or together) fed birds as compared to the birds in control birds (P<0.05). No effects of the amino acids was observed on blood enzymes, thyroid hormones, anus temperature and the blood indices at day 42 of age (P>0.05). CONLUSIONS: taken together, tryptophan and threonine decreases the feed consumption and feed conversion ratio and consequently decrease the ascites mortality in broiler chickens.Shahid Chamran University of AhvazIranian Veterinary Journal1735-687313420180121Developmental trend of the neurovertebral growth changes in the spine of the male and female goat fetuses (Capra hircus)Developmental trend of the neurovertebral growth changes in the spine of the male and female goat fetuses (Capra hircus)67735767110.22055/ivj.2017.53539.1730FASeyed Morteza GhaziPhD Graduated in Comparative Veterinary Anatomy and Embryology, Department of Basic Sciences, Faculty
of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IranReza RanjbarAssociate Professor of Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran
University of Ahvaz, Ahvaz, Iran0000-0002-3213-1684Mahmoud Khaksary MahabadyAssociate Professor of Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran
University of Ahvaz, Ahvaz, IranJournal Article20160807. Total and regional length of spinal cord and vertebral column were studied in 36 goat fetuses, collected from the Ahvaz slaughterhouse. These specimens were assigned into 3 groups, group1 (CRL,10-20 cm), group2 (20-30 cm) and group3 (30-40 cm). Each group consisted of 12 fetuses (6 male and 6 female). Observations in all 3 groups revealed that in the spinal cord the longest region was belong to the thoracic region, followed by cervical, lumbar, sacral and coccygeal, while in vertebral column the length of sacral region was shorter than that of coccygeal. Statistically, there were no significant differences in either spinal cord and vertebral column regions according to the sex of fetuses, while there were significant differences (P<0.001) between all regions in either spinal cord and vertebral column of all 3 groups. Normal vertebral formula in the male and female goat fetuses was C7T12-13L6-7S5Co10-12 Except in cervical region, the number of vertebrae in other regions were in agree and disagree with the records appeared in certain anatomical books.. Total and regional length of spinal cord and vertebral column were studied in 36 goat fetuses, collected from the Ahvaz slaughterhouse. These specimens were assigned into 3 groups, group1 (CRL,10-20 cm), group2 (20-30 cm) and group3 (30-40 cm). Each group consisted of 12 fetuses (6 male and 6 female). Observations in all 3 groups revealed that in the spinal cord the longest region was belong to the thoracic region, followed by cervical, lumbar, sacral and coccygeal, while in vertebral column the length of sacral region was shorter than that of coccygeal. Statistically, there were no significant differences in either spinal cord and vertebral column regions according to the sex of fetuses, while there were significant differences (P<0.001) between all regions in either spinal cord and vertebral column of all 3 groups. Normal vertebral formula in the male and female goat fetuses was C7T12-13L6-7S5Co10-12 Except in cervical region, the number of vertebrae in other regions were in agree and disagree with the records appeared in certain anatomical books.Shahid Chamran University of AhvazIranian Veterinary Journal1735-687313420180121Histophysiological study of thyroid gland in Liza abu exposed to Musa creek pollutionHistophysiological study of thyroid gland in Liza abu exposed to Musa creek pollution74855767210.22055/ivj.2017.37522.1622FANeda KoushaMSc Graduated of Histology, Faculty of Marine Science, Khorramshahr University of Marine Science
and Technology, Khorramshahr, IranNegin SalamatAssociate Professor, Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of
Marine Science and Technology, Khorramshahr, IranMohamad Taghi RonaghAssisstant Professor, Department of Marine Biology, Faculty of Marine Science, Khorramshahr University
of Marine Science and Technology, Khorramshahr, IranAbdolali Movahedi NiyaAssociate Professor, Department of Marine Biology, Faculty of Marine Science, University of Mazandaran,
Babolsar, IranJournal Article20160422The hormones of pituitary-thyroid axis play an important role in the maintaining of the normal physiological status of the body in vertebrates. Therefore, disturbance in the function of this axis can affect other parts of the body. In the present study, 50 male and female Liza abu (45.51 ± 8.2g body weight and 16.25 ± 0.88cm body length) were collected from 5 stations in the Musa creek including: 1) Petrochemical 2) Ghanam 3) Zangi 4) Doragh and 5) Patil stations. 10 L. abu were also caught at Sajafi station (located in the Musa creek) as a control site. Bleeding was carried out and the blood plasma was separated. Then, the plasma levels of thyroid hormones (triiodothyronine (T3) and tetraidothyronin (T4)) were measured using ELISA method. Tissue samples were also taken from thyroid and were fixed in the 15% formalin and prepared according routine histological process. The less and highest amounts of T3 and T4 hormones respectively were measured in the fish plasma from Patil station. The most histopathological changes (such as hemorrhage) were observed in the fish from Petrochemical station. The results of this study indicated the adverse effects of environmental pollution on the balance of thyroid hormones.The hormones of pituitary-thyroid axis play an important role in the maintaining of the normal physiological status of the body in vertebrates. Therefore, disturbance in the function of this axis can affect other parts of the body. In the present study, 50 male and female Liza abu (45.51 ± 8.2g body weight and 16.25 ± 0.88cm body length) were collected from 5 stations in the Musa creek including: 1) Petrochemical 2) Ghanam 3) Zangi 4) Doragh and 5) Patil stations. 10 L. abu were also caught at Sajafi station (located in the Musa creek) as a control site. Bleeding was carried out and the blood plasma was separated. Then, the plasma levels of thyroid hormones (triiodothyronine (T3) and tetraidothyronin (T4)) were measured using ELISA method. Tissue samples were also taken from thyroid and were fixed in the 15% formalin and prepared according routine histological process. The less and highest amounts of T3 and T4 hormones respectively were measured in the fish plasma from Patil station. The most histopathological changes (such as hemorrhage) were observed in the fish from Petrochemical station. The results of this study indicated the adverse effects of environmental pollution on the balance of thyroid hormones.Shahid Chamran University of AhvazIranian Veterinary Journal1735-687313420180121Phenotypic and genotypic resistance of Escherichia coli isolated from broiler chickens of Urmia to sulfonamidesPhenotypic and genotypic resistance of Escherichia coli isolated from broiler chickens of Urmia to sulfonamides86915767310.22055/ivj.2017.37287.1618FASina Mojaver RostamiDVM Graduated from Faculty of Veterinary Medicine, Urmia University, Urmia, IranAbolfazl GhanieiAssistant Professor, Department of Poultry Diseases, Faculty of Veterinary Medicine, Urmia University,
Urmia, IranVahid MohammadiAssistant Professor, Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine,
Urmia University, Urmia, IranJournal Article20160515Colibacilosis is one of the most important infectious diseases in poultry industry. Antibiotics are the most important tool in dealing with the treatment and control of the disease. Checking the sensitivity of bacterial isolates to antimicrobial compounds, before choosing an antibiotic drug in the region is essential. In this study, phenotypic resistance profile of 44 isolates of Escherichia coli recovered from broiler chickens of Urmia city suspected to colisepticemia to Sulfadiazine evaluated by disk diffusion method. Genotypic resistance profile was also assessed by Polymerase Chain Reaction (PCR) of Sul1 gene. Results showed that 20 (45.5%) Escherichia coli isolates were resistant to sulfadiazine and Sul1 gene was detected in 25 (56.8%) isolates. Five isolates that had the Sul1 gene showed no antibiotic resistance in antibiogram test. The results showed that the resistance rate of isolates to sulfonamides was high and sul1 gene had a major role in sulfadiazine resistance of these isolates.Colibacilosis is one of the most important infectious diseases in poultry industry. Antibiotics are the most important tool in dealing with the treatment and control of the disease. Checking the sensitivity of bacterial isolates to antimicrobial compounds, before choosing an antibiotic drug in the region is essential. In this study, phenotypic resistance profile of 44 isolates of Escherichia coli recovered from broiler chickens of Urmia city suspected to colisepticemia to Sulfadiazine evaluated by disk diffusion method. Genotypic resistance profile was also assessed by Polymerase Chain Reaction (PCR) of Sul1 gene. Results showed that 20 (45.5%) Escherichia coli isolates were resistant to sulfadiazine and Sul1 gene was detected in 25 (56.8%) isolates. Five isolates that had the Sul1 gene showed no antibiotic resistance in antibiogram test. The results showed that the resistance rate of isolates to sulfonamides was high and sul1 gene had a major role in sulfadiazine resistance of these isolates.Shahid Chamran University of AhvazIranian Veterinary Journal1735-687313420180121The study of the effect of drug interactions of phenylbutazone on pharmacokinetics parameters of thiopental sodium in female dogThe study of the effect of drug interactions of phenylbutazone on pharmacokinetics parameters of thiopental sodium in female dog92995767410.22055/ivj.2017.24855.1530FASaeed MoradinejadeDVM, Faculty of Veterinary Medicine, Sanandaj Branch, Islamic Azad University, Sanandaj, IranMahdieh RaeeszadehAssistant Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Sanandaj Branch, Islamic
Azad University, Sanandaj, IranHamid RajaianProfessor, Department of Basic Sciences, Faculty of Veterinary Medicine, Shiraz University, Shiraz, IranJournal Article20161105Pharmacokinetics drug interactions are one of the most important drug interactions in clinical pharmacology science which can consequently affect the pharmacological activities of drugs. The aim of this research study was to investigate the effect of phenylbutazone on various pharmacokinetics parameters of thiopental in drug interactions in female dogs. On this basis, eight female dogs were randomly divided into two groups (control and test groups). The Biochemical and hematological parameters were measured and were found to be at normal range. Normal saline of 0.9% (0.1 ml/kg) and thiopental sodium of 5% (0.34 ml/kg) were intravenously administered to animals in the control group. Equivalent dose of thiopental sodium was also administered following the injection of phenylbutazone 20% via the same route. Serum was separated by centrifugation and thiopental concentration was measured using HPLC method. Pharmacokinetics factors including rate of elimination, half-life, volume of distribution, and clearance were calculated using non-compartmental analysis of the concentration-time curves. The results of this study showed that in comparison with the control group, half-life, distribution volume of thiopental were significantly increased in the test group (P<0.05). ِElimination rate of the thiopental and clearance was also reduced in test group compared to control group and the difference was significant (P<0.05). Therefore, phenylbutazone can increase half-life and volume of distribution of thiopental in female dog.Pharmacokinetics drug interactions are one of the most important drug interactions in clinical pharmacology science which can consequently affect the pharmacological activities of drugs. The aim of this research study was to investigate the effect of phenylbutazone on various pharmacokinetics parameters of thiopental in drug interactions in female dogs. On this basis, eight female dogs were randomly divided into two groups (control and test groups). The Biochemical and hematological parameters were measured and were found to be at normal range. Normal saline of 0.9% (0.1 ml/kg) and thiopental sodium of 5% (0.34 ml/kg) were intravenously administered to animals in the control group. Equivalent dose of thiopental sodium was also administered following the injection of phenylbutazone 20% via the same route. Serum was separated by centrifugation and thiopental concentration was measured using HPLC method. Pharmacokinetics factors including rate of elimination, half-life, volume of distribution, and clearance were calculated using non-compartmental analysis of the concentration-time curves. The results of this study showed that in comparison with the control group, half-life, distribution volume of thiopental were significantly increased in the test group (P<0.05). ِElimination rate of the thiopental and clearance was also reduced in test group compared to control group and the difference was significant (P<0.05). Therefore, phenylbutazone can increase half-life and volume of distribution of thiopental in female dog.Shahid Chamran University of AhvazIranian Veterinary Journal1735-687313420180121Comparison of the performance between three strains of broiler chicks in IranComparison of the performance between three strains of broiler chicks in Iran1001085767510.22055/ivj.2017.46719.1676FAMansour MayahiProfessor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of
Ahvaz, Ahvaz, IranForough TalazadehAssociate Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran
University of Ahvaz, Ahvaz, IranMahya AbdolshahDVM Graduated from Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IranJournal Article20160311In order to compare performance in three commercial broiler strains, 36ooo day-old broiler chicks Hubbard f15, Cobb-500 and Ross308, were divided into 3 equal groups with 2 replicates and 6000 birds per replicate. The birds were reared under similar conditions from 1 day-old to 49 days of age. The performances of birds including weight gain, feed intake, feed conversion ratio (FCR), livability and flocks production index were measured. Results showed that there was significant differences in weekly weight gain among hybrids (p < 0.05), and the weight of Hubbard F15 was higher than others till the end of 49 days, while cobb500 had the lowest weight gain. Birds feed intake during experiment period was significantly different (p < 0.05) and Hubbard F15 had the lowest feed intake and Cobb500 had the highest feed intake. Feed conversion ratio between birds was significantly different (p < 0.05) and Hubbard F15 had the lowest FCR while cobb500 had the highest. There was significant differences in livability ratio among the hybrids, The lowest mortality rate in the trial was recorded in Hubbard genotype (p<0.05). There was significant differences in production index and Hubbard F15 had the highest production index while cobb500 had the lowest one.In order to compare performance in three commercial broiler strains, 36ooo day-old broiler chicks Hubbard f15, Cobb-500 and Ross308, were divided into 3 equal groups with 2 replicates and 6000 birds per replicate. The birds were reared under similar conditions from 1 day-old to 49 days of age. The performances of birds including weight gain, feed intake, feed conversion ratio (FCR), livability and flocks production index were measured. Results showed that there was significant differences in weekly weight gain among hybrids (p < 0.05), and the weight of Hubbard F15 was higher than others till the end of 49 days, while cobb500 had the lowest weight gain. Birds feed intake during experiment period was significantly different (p < 0.05) and Hubbard F15 had the lowest feed intake and Cobb500 had the highest feed intake. Feed conversion ratio between birds was significantly different (p < 0.05) and Hubbard F15 had the lowest FCR while cobb500 had the highest. There was significant differences in livability ratio among the hybrids, The lowest mortality rate in the trial was recorded in Hubbard genotype (p<0.05). There was significant differences in production index and Hubbard F15 had the highest production index while cobb500 had the lowest one.Shahid Chamran University of AhvazIranian Veterinary Journal1735-687313420180121Simultaneous occurrence of strangle and glanders diseases in a mule in Ilam province, IranSimultaneous occurrence of strangle and glanders diseases in a mule in Ilam province, Iran1091145767710.22055/ivj.2017.56495.1758FAAli Abbas NikvandAssistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran
University of Ahvaz, Ahvaz, IranMehdi RashnavadiAssistant Professor, Faculty of Veterinary Medicine, Ilam University, Ilam, IranNader MosavariAssociate Professor, Animal Tuberculosis central Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension, Karaj, Iran0000-0002-3480-3376Roholla KeshavarzExpert of Animal Tuberculosis Reference Laboratory, Razi Vaccine and Serum Research Institute,
Agricultural Research Education and Extension, Karaj, IranRoholah DelkhahExpert of Hygiene and Animal Diseases Management Office, Veterinary Directorate General of Tehran Province, Iranian Veterinary Organization, Tehran, IranJournal Article20160825Abstract<br /> Glanders is a contagious infection of odd-toe ungulates that is occurs in three forms of skin, nasal and pulmonary. It is caused by bacterium Burkholderia mallei. Horses generally affected to chronic form, whereas often takes as acute and pernicious in donkey and mule. In June 2015, a 5- year- old male mule was referred to veterinary services with complains of inappetence, skin lesion and nasal purulent discharges, in Ilam province. Subcutaneous nodules and cutaneous ulcers, bilateral nasal mucosal ulcers with discharge and snoring were noted during examination. Initially, for suspecting to glanders disease, mallein test was done but its result was negative. Then, nasal and skinny unopened nodule swabs with serum sample were evaluated with microbiology, Complement Fixation Test and Elisa for differentiating of glanders from strangle and ulcerative lymphangitis diseases. Bacterium Burkholderia mallei was not isolated from cultured swabs, but Streptococcus equi isolated. The results of serum Complement Fixation Test and Elisa were also positive for glanders. In conclusion rely on negative mallein test, that is common in clinical cases and negative culture of Burkholderia mallei due to time consuming submit process of bacteriological sample to few individual glanders laboratories may be misleading for glanders diagnosis, but in present case, simultaneous diagnosis of strangle and glanders diseases was made base on combination of clinical signs, positive serum complement fixation test and indirect Elisa for glanders associated with separating of Streptococcus equi bacterium.Abstract<br /> Glanders is a contagious infection of odd-toe ungulates that is occurs in three forms of skin, nasal and pulmonary. It is caused by bacterium Burkholderia mallei. Horses generally affected to chronic form, whereas often takes as acute and pernicious in donkey and mule. In June 2015, a 5- year- old male mule was referred to veterinary services with complains of inappetence, skin lesion and nasal purulent discharges, in Ilam province. Subcutaneous nodules and cutaneous ulcers, bilateral nasal mucosal ulcers with discharge and snoring were noted during examination. Initially, for suspecting to glanders disease, mallein test was done but its result was negative. Then, nasal and skinny unopened nodule swabs with serum sample were evaluated with microbiology, Complement Fixation Test and Elisa for differentiating of glanders from strangle and ulcerative lymphangitis diseases. Bacterium Burkholderia mallei was not isolated from cultured swabs, but Streptococcus equi isolated. The results of serum Complement Fixation Test and Elisa were also positive for glanders. In conclusion rely on negative mallein test, that is common in clinical cases and negative culture of Burkholderia mallei due to time consuming submit process of bacteriological sample to few individual glanders laboratories may be misleading for glanders diagnosis, but in present case, simultaneous diagnosis of strangle and glanders diseases was made base on combination of clinical signs, positive serum complement fixation test and indirect Elisa for glanders associated with separating of Streptococcus equi bacterium.