Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
13
2
2017
06
22
Effects of Cholesterol loaded cyclodextrin on vitrification of the cat epididymal sperm
5
12
FA
Maryam
Rahbar
دانش آموخته دکترای حرفه ای دامپزشکی، دانشگاه شهید چمران، اهواز ایران
maryam.rahbar68@gmail.com
Farid
Barati
0000-0003-3773-019X
Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
fabrtir@yahoo.com
Ghodratollah
Mohammadi
گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمرا، اهواز، ایران
ghmohammadi9@yahoo.com
Bahman
Mosallanejad
گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران، اهواز، ایران
mosallanejad@gmail.com
10.22055/ivj.2017.46882.1679
Cholesterol loaded cyclodextrin (CLC) efficiently protected sperm of some species against cryodamage. On the other hand, vitrification of sperm especially in human opened a new horizon in the field of sperm freezing. The aim of the present study was to evaluate the effect of CLC on vitrification of cat sperm. The tom epididymal sperm (n=22 pairs) was extracted from epididymides and subjected to vitrification in TRIS based solution that was incorporated with or without CLC. The warmed sperm was analyzed for motility, viability and morphology. Vitrification significantly (P<0.0001) reduced the sperm motility (%) in CLC-plus (3.6±2.28) and CLC-minus (1.1±2.02) compared to the fresh samples (28.3±1.82). Sperm viability was also significantly decreased the following vitrification either with CLC (80.6±2.44) or without CLC (78.2±2.15) compared to the fresh samples (89.9±1.94; P=0.0011). In spite of the significant impact of vitrification on cat epididymal sperm, it seems that there is an indigenous tolerance to the vitrification procedure and CLC did not have more beneficial effect on it.
Cat,Cyclodextrin,Epididymal sperm,Vitrification
https://www.ivj.ir/article_48595.html
https://www.ivj.ir/article_48595_a8cecf21b5162d2ce0b94cf3622e13b4.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
13
2
2017
06
22
concentrations of Royal Jelly supplementation in tris diluent on semen quality parameters of Arabi ram
13
21
FA
soraya
Raeesi Dehkohneh
Graduate student of Animal Physiology
soraya.raeesi@gmail.com
Morteza
mamouei
Associate Professors, Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran
mamouei_m@yahoo.com
saleh
tabatabaei
Assistant Professors Department of Animal Sciences, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran
s_tabatabaei58@yahoo.com
Jamal
Fayazi
Associate Professors, Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran
j_fayazi@yahoo.com
hessam
naghibi rokni
MSc of Animal Physiology, Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran
rokni_hessam@yahoo.com
10.22055/ivj.2017.30765.1564
This experiment was conducted to investigate the effects of different concentrations of royal jelly in tris extender on motility, viability, morphological abnormalities and membrane integrity of spermatozoa in Arabi rams. For this purpose, the semen of 10 Arabi rams were polled and then examined under 5 treatments including the tris diluent containing the concentrations of zero, 0.5, 1, 1.5 and 2% royal jelly in three times of zero, 24 and 48 hours in a completely randomized design. The results showed a significant difference in motility of spermatozoa among royal jelly treatments and control (P<0.05). At 0.5, 1 and 1.5% of royal jelly, sperm motility was significantly more than the control group (P<0.05). Sperm viability rate was not affected by treatments. The used concentrations of royal jelly have not significantly effect on the amount of morphological abnormalities of Arabic ram spermatozoa in comparison with control group. Between different concentrations of royal jelly treatments and control group, there was not significant difference in the sperm plasma membrane integrity (P>0.05). Concentrations 1 and 1.5% of royal jelly improved the amount of sperm plasma membrane integrity when compared with 0.5 and 2% of royal jelly concentrations (P<0.05). In general, according to this study, it is concluded that the low levels of royal jelly (0.5, 1 and 1.5%) improved the sperm progressive motility during liquid storage in Arabi ram.
Arabi ram,Royal Jelly,Sperm,Tris diluent,Quality parameters
https://www.ivj.ir/article_48599.html
https://www.ivj.ir/article_48599_e6b102df523e0b95d8f606d1dbc1160d.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
13
2
2017
06
22
Study of polymorphism in intron 4 and exon 5 of Ghrelin gene in Khouzestan native chickens using PCR-RFLP
22
28
FA
S
Rezaei Yazd Abadi
MSc Gradueted of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran
rezaeisaeed64@gmail.com
H
Roshanfekr
Associate Professor, Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran
roshanfek_hd@yahoo.com
M.T.
Beigi Nasiri
Professor, Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran
mt_nassiri@yahoo.com
J
Fayazi
Associate Professor, Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran
j_fayazi@yahoo.com
10.22055/ivj.2017.43262.1632
Abstract<br /> <br /> This study was performed in order to identify polymorphism in fourth intron and fifth exon in Khuzestan province native chicken’s ghrelin gene using PCR-RFLP method. Therefore, blood sampling from 100 chickens of Native chickens in Khuzestan province breeding and Jihad livestock inputs randomly was performed. DNA extraction from blood samples was performed using salt method. The quantity and quality of DNA was determined using gel electrophoresis (1%) and Nanodrop respectively and then 228 bp fragment of fourth intron and fifth exon region was amplified using polymerase chain reaction (PCR). Eventually amplified samples were digested with HaeIII enzyme and digest parts of 173 and 55 bp were found. The results showed that in all the samples after enzymatic digestion was only a same band shape therefore, considering the same genotype samples examined in this study, mutation was not founded. Not found polymorphism may be due to closed nurture environment and the low number of samples.
Khouzestan native chickens,Ghrelin gene,PCR-RFLP
https://www.ivj.ir/article_48600.html
https://www.ivj.ir/article_48600_e392f5b457708c7c83bfb0162224ab40.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
13
2
2017
06
22
Comparison of ELISA method, PCR and Ziehl-Neelsen staining of the rectal mucosa for detection of Mycobacterium avium subsp. paratuberculosis infection in cattle
29
37
FA
Mehdi
Zarei
Associate Professor, Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
zarei@scu.ac.ir
Masoud
Ghorbanpour
0000000203014577
Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
m.ghorbanpoor1967@gmail.com
S
Tajbakhsh
MSc Graduated of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
samanetajbakhsh@yahoo.com
N
Mosavari
Assistant Professor, Department of Bacteriology, Razi Vaccine and Serum Research Institute, Hessarak, Karaj, Iran
nadermosavari@yahoo.com
10.22055/ivj.2017.47784.1690
Mycobacterium avium subsp. paratuberculosis is the etiologic agent of Johne’s disease in cattle and other ruminants. This disease is considered the most extensive, in terms of incidence, and the most important disease, in terms of economic importance, in the animal husbandry industry. Rapid and accurate diagnosis of disease is extremely important in preventing the spread of disease in the herd. In the present study, the prevalence of Mycobacterium avium subsp. paratuberculosis infection in slaughtered cows in Ahvaz slaughterhouse was evaluated and compared using three methods, rectal scraping PCR, acid-fast staining and serum ELISA. In this study, blood and rectal scraping samples were collected from 200 cows. Serum ELISA was performed with a commercial ELISA kit, Ziehl-Neelsen staining was done based on conventional method, and PCR was performed using IS900 and hspX specific primers for Mycobacterium avium subsp. paratuberculosis. Rectal scraping PCR detected 27 of 200 cows (13.5%) infected with Mycobacterium avium subsp. paratuberculosis, acid-fast staining of the rectal mucosa detected 8 cows (4%), and serum ELISA detected only 6 cows (3%). Comparing the results of these three tests shows moderate agreement between acid-fast staining and rectal scraping PCR tests (kappa score, 0.42), low agreement between ELISA vs. rectal scraping PCR (kappa score, 0.08), and ELISA vs. acid-fast staining (kappa score, 0.11). According to the results of the present study, although the rectal scraping PCR showed better results in rapid diagnosis of the infected cows, but in order to achieve a more precise result for rapid diagnosis of the infected cows and also in epidemiological studies for determining the prevalence of Mycobacterium avium subsp. paratuberculosis infection, simultaneous conduct of rectal scraping PCR and serum ELISA can be recommended.
Johne's disease,Mycobacterium avium subsp. paratuberculosis,Ziehl-Neelsen
https://www.ivj.ir/article_48601.html
https://www.ivj.ir/article_48601_3ca759a39c74fc7d33db57864d00465f.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
13
2
2017
06
22
Effects of gamma-irradiated Ichthyophthirius multifiliis coated with calcium phosphate nanoparticles on immunological responses in rainbow trout (Oncorhynchus mykiss) skin mucus
38
47
FA
N
Sheikhzadeh
Associate Professor, Department of Food Hygiene and Aquatic Animals, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
nsheikh@tabrizu.ac.ir
M
Heidarieh
Assistant Professor, Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, Karaj, Iran
mheidarieh@nrcam.org
M
Falsafi
DVM Graduated from Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
falsafi.monireh@yahoo.com
K
Nofouzi
Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
nfouzi@tabrizu.ac.ir
A
Tukmechi
Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
atokmachi@gmail.com
10.22055/ivj.2017.52760.1721
Current study was performed to prepare a radiovaccine against Ichthyophthirius multifiliis, the causative agent of white spot in fresh water fishes. After irradiation with gamma ray, Ichthyophthirius multifiliis parasites were coated with calcium phosphate nanoparticles and then effects of prepared vaccine on non-specific immune system in rainbow trout skin mucus were evaluated. After 10-day adaption period, fish with mean weight 30 gram were distributed to 4 groups: Three groups were bath-vaccinated with gamma-irradiated Ichthyophthirius multifiliis coated with nano calcium phosphate, gamma-irradiated Ichthyophthirius multifiliis or nano calcium phosphate alone. In control group, no additives were used. On day 20, skin mucus from treated fish was analyzed for evaluating the innate immune parameters. Results showed that lysozyme and alkaline phosphatae activities and hemagglutination titer of fish treated with gamma-irradiated Ichthyophthirius multifilis coated with calcium phosphate nanoparticle were higher than other groups. But esterase activity in treatment groups did no change in comparison with the control group. It can be assumed that use of calcium phosphate nanoparticle formulated in gamma-irradiated Ichthyophthirius multifilis trophonts could enhance the innate immunity components in rainbow trout skin mucus. Further studies are needed to evaluate the effects of this radiovaccine on specific immune responses in rainbow trout.
Ichthyophthirius multifiliis,Vaccine,mucosal immunity,rainbow trout
https://www.ivj.ir/article_48602.html
https://www.ivj.ir/article_48602_b4cdb1cbe0201fefb2081db195018fe4.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
13
2
2017
06
22
Histomorphometrical effects of Aloe Vera on diabetic rat adrenal gland
48
57
FA
N
Erfani Majd
Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
naeemalbo@yahoo.com
N
Sadeghi
MSc Graduated of Histology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
n.sadeghi259@gmail.com
Sh
Hosseinifar
0000-0001-6935-7364
Assistant Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
sh.hosseinifar@scu.ac.ir
10.22055/ivj.2017.28887.1550
Aloe vera has many applications in traditional medicine .In present study, the hypoglycemic effects of the Aloe vera and its protective effects on the adrenal gland on diabetic rats were studied. 50 mature male rats (2-3 months old) and weighing 200-250 were divided randomly into 5 groups (10 rats in each groups) as following: Group I (control), has not received any treatment. Group II (Diabetic) which received 65 mg/kg Streptozotocin (IP). and rats with blood glucose more than 250 mg/dL were considered as diabetic. Group III, diabetic rats which received the Aloe vera gel with dose of 400 mg/kg daily. Group IV, diabetic rats which received insulin in 10 units/rat. Group V,received Aloe vera gel only. On days 15 and 30 of experiment, blood glucose of all groups were measured. Animals were euthanized with ether. Then, tissues samples were collected from adrenal glands, and fixed in 10% neutral buffered formalin solution. The sections were made using routine paraffin embedding method and stained by haematoxylin-eosin (H & E). Histomorphometrical parameters were evaluated using Digital Dino-Lite lens and Dino capture software 1. The results showed that thickness of fasciculata layer and the size of the spongiocyte were increased significantly in diabetic rat (P≤0. 05) but the thickness of fasciculata layer was decreased significantly in diabetic rats which received Aloe vera and insulin (P≤0. 05). The blood glucose was decreased significantly in diabetic rats. Conclusion, Aloe vera can be has beneficial and protective effects on adrenal glands in diabetic rats.
Aloe vera,Diabetes,Adrenal gland,Histomorphometry,Rat
https://www.ivj.ir/article_48603.html
https://www.ivj.ir/article_48603_e31bfad77b7276e0e3c7ad2da1a49612.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
13
2
2017
06
22
Evaluation of clinical and subclinical hypocalcemia in primiparous and multiparous cows consumed limited calcium and phosphorus diet in close up period
58
68
FA
K
Faramarzian
DVSc Graduated of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
faramarzian85@yahoo.com
Mohammad Rahim
Haji Hajikolaei
0000-0001-8964-8820
Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
mhajih@scu.ac.ir
M
Nouri
Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
m.nouri@scu.ac.ir
m
Mohebbi Fani
0000-0002-7726-1255
Professor, Department of Animal Health Management, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran
mohebbi@shirazu.ac.ir
A
Shahriari
Associate Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
a.shahriari@scu.ac.ir
10.22055/ivj.2017.48689.1701
Clinical and subclinical hypocalcemia are two factors of economic losses in herds, and occurs when dairy cows are unable to replace the calcium lost through colostrum and milk secretion. This study was performed to evaluate occurrence of milk fever, subclinical hypocalcemia and calcium related diseases in high yielding dairy cows. In this study, blood samples of 18 primiparous and 18 multiparous cows in transition period in two farms with the same management status were taken. Dairy cows received limited calcium and phosphorus diet, from 21 days prepartum until parturition and lactation diet according to nutritional requirements after parturition. Serum level of calcium, phosphorous and magnesium were measured with routine laboratory methods. Base on cut point of calcium (8.5 mg/kg), subclinical hypocalcemia in primiparous and multiparous cows before parturition was 16.7 and after parturition was 11.1 and 16.7 %. The frequency of milk fever, retained fetal membranes, metritis and displaced abomasum of all parturition in 1 year were 1.3, 12.6, 5.9 and 0 percent, respectively. Results show that parity dosn’t have significant effect on subclinical hypocalcemia occurrence in cows that consumed limited calcium and phosphorus diet in close up period. Also regardless of parity limited calcium and phosphorus diet in close up period have a positive effect on prevention of milk fever.
: Calcium,Phosphorus,Magnesium,Hypocalcemia,Cow
https://www.ivj.ir/article_48604.html
https://www.ivj.ir/article_48604_209b9c864a93e6c5377b6340c519f0c8.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
13
2
2017
06
22
Changes of testosterone serum concentration in two seasons warm and cold in dogs of Ahvaz district
69
77
FA
S
Gooraninejad
Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
goorani_s@scu.ac.ir
Bahman
Mosallanejad
Associate Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
bmosallanejad@scu.ac.ir
F
Barati
Associate Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
baratif@scu.ac.ir
m
Shahi Khesht
DVM Graduated from Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
dvmmohsen@yahoo.com
10.22055/ivj.2017.43107.1628
Photoperiod and environmental temperature are two important factors that may influence the reproductive activity of various species. As regards the ambient temperature goes up 50 ˚C in warm months of Khuzestan province, so the aim of the present survey was evaluation of the different season's effect (warm and cold) on testosterone serum concentration in native dogs in Ahvaz district. For this purpose, six adult male dogs, clinically healthy and with age 1.5-2 years old were selected. Blood samples were collected during one year, the middle of each month and in the period between hours 8.30 to 11.30 mornings (72 samples). Testosterone concentration was measured using ELISA technique in serum samples. The obtained results were analyzed by repeated measure ANOVA and Pearson correlation. In this survey, mean±standard error of testosterone serum concentration was detected 4.5±1.8 ng/ml yearly. The minimum and maximum of testosterone concentration were 0.2 and 7.3 ng/ml, respectively. The results showed that the changes of serum testosterone level, were significantly influenced by the sampling month (P<0.0001), so that the lowest levels of testosterone concentration was in May and June (1.7 ng/ml), while the highest level was in January and August (6.7 and 6.8 ng/ml) respectively. The mean testosterone concentration was 4.12±0.25 ng/ml in the warm season and 4.87±0.16 ng/ml in the cold season. The difference was statistically significant between the two seasons (P<0.05). In general, the season variables, have the potential effect on testosterone concentration, that is, the temperature and daylight duration correlated negatively with the testosterone serum concentration (temperature R=- 0.27; P=0.001, daylight duration R=- 0.20; p=0.013), and the humidity level, had a positive significant correlation with testosterone concentration (except August) in the studied dogs (R=0.21; P=0.011). In conclusion, it can be stated that the testosterone concentration is affected by the sampling season.
testosterone,season,Dog,Ahvaz
https://www.ivj.ir/article_48605.html
https://www.ivj.ir/article_48605_0aa2a662850e7c20e13b82180bd1e53b.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
13
2
2017
06
22
Histological and histochemical study of Spleen in Argyrosomus hololepidotus
78
85
FA
Hassan
Morovvati
0000-0003-0275-1636
Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
hmorovvati@ut.ac.ir
Salman
Soltani
PhD Student of Histology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
soltani1981@yahoo.com
Mohammad Taghi
sheybani
Associate Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
tsheibani@yahoo.com
Masoud
Adibmoradi
Associate Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
adibmoradi@ut.ac.ir
10.22055/ivj.2017.48094.1694
Argyrosomidae family and the species Argyrosomus hololepidotus due to having high protein levels, are among the most valuable aquatics in the Persian Gulf, Oman Sea and the coasts of Khuzestan. Lymphoid tissues are of the most important tissues in fish which their evaluation is important in health and disease investigation. Unlike mammals, fish lack lymph nodes and also there is not hematopoietic tissue in their bone marrows, so hematopoiesis is done in their spleen and kidney. In this study, 6 Argyrosomus hololepidotus were provided from the Persian Gulf. Tissue samples from the spleen with 5mm thickness were prepared and fixed in 10% buffered formalin. Then tissue sections were prepared in 5-6 μ and after H&E staining, the structures of the capsule and parenchyma of spleen were studied histologically. In order to confirm the results and findings of this staining, some other specific stainings such as PAS, Masson trichrome and silver staining were used. Microscopic results showed that tissue structure of spleen parenchyma was consisted of white pulp, red pulp, melano-macrophage centers and ellipsoid capillaries. Much of the red pulp is occupied by sinusoidal capillaries full of erythrocyte, which indicates the spleen in Argyrosomus hololepidotus serves as a source of blood reservoir.
Argyrosomus hololepidotus,histochemistry,Melano-macrophage,spleen
https://www.ivj.ir/article_48606.html
https://www.ivj.ir/article_48606_36fa384043eca8414a937b50f6a5d032.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
13
2
2017
06
22
Effect of walnut green husk (Juglans regia) powder on immune responses of broiler chickens
86
95
FA
B
Mousavi Razi
MSc Student of Animal Physiology, Faculty of Agriculture Science, University of Guilan, Rasht, Iran
b.mrazii@gmail.com
Mohammad
Roostaei Ali Mehr
Associate Professor, Department of Animal Science, Faculty of Agriculture, University of Guilan, Rasht, Iran
roostaei@guilan.ac.ir
M
Mohiti Asli
Assistant Professor, Department of Animal Science, Faculty of Agriculture, University of Guilan, Rasht, Iran
mmohiti@gmail.com
10.22055/ivj.2017.50827.1712
Experiment was conducted to evaluate the effect of green husk of walnut powder on immune responses, by using 200 broilers. Birds were split into five groups and each group into four sub groups which include 10 birds. The levels of zero, 1, 2, 3 and 4 % powder of green husk of walnut were added to diet of each group. Intramuscular injection of 0.1 mL of the 25% SRBC suspension was performed at 8 and 22 d and the titers of IgG and IgM was determined by haemagglutination test at 21, 28, 35 and 42 d. Blood samples were collected to determine count of red and white blood cells and after that birds were slaughter to evaluate weight of visceral fat and organelles at 42 d. Titer IgM was higher in treated birds with 4 % green husk (2.93) than control (2.12) at 21 d (P<0.05). Titer IgG was higher in treated birds with 4 % green husk (5.5), 3% green husk (5.25) and 2% green husk (4.81) than control (3.68) at 42 d (P<0.05). Ratio of hetrophil to lymphocyte and percentage of heterophil were lower in treated birds with 4 % green husk (0.55 and 33.23 respectively) than control (P<0.05). Percentage of lymphocytes was higher in treated birds with 4 % green husk (59.96) than control (56.87; P<0.05). The highest percentage relative weight of bursa was observed in treated birds with 2 (0.17), 3 (0.17) and 4% green husk (0.16; P<0.05). The percentage relative weight of thymus was higher in treated birds with 3% green husk (0.59) and 4% green husk (0.54) than control (P<0.05). Therefore, supplementation diet with green husk of walnut powder improved function of broiler immune system.
broiler,Green husk of walnut,immune system
https://www.ivj.ir/article_48607.html
https://www.ivj.ir/article_48607_d8e26160daf60943b8c1f83d069b7276.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
13
2
2017
06
22
The use of fascia lata as an autograft for permanent treatment of anterior cruciate ligament ruptures with extra capsular technique in dogs; New Surgical Technique
96
111
FA
H
Naddaf
Associate Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
hdnaddaf@scu.ac.ir
A
Ghadiri
Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
alighadiri@scu.ac.ir
A.R.
Imani
DVSc Graduated of Veterinary Surgery, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
imaniamirreza@yahoo.com
10.22055/ivj.2017.34365.1588
Cruciate ligament rupture is one of the main reasons of lameness in dogs. Standard techniques based on differences in patient selection criteria has not been determined yet and is still in question. The aim of this study is to provide a new technique that uses the strength of the fascia lata, passing it through the regular isometric knee and creating mutual stability. A total of 10 adult male dogs weighing between 14-20 kg of mixed breed were randomly divided into two equal groups, MRIT and fascia lata (FLG). Under general anesthesia and cruciate ligament was resected. Cruciate ligament rupture in FLG was stabilized Using fascia lata and creating channels in to the proximal tibia and distal femur. In MRIT group, stabilization was also established in the usual way. In order to evaluate and compare the two methods, following parameters were scored in 16 Consecutive weeks, drawer movement, weight gain and lameness in walk and trot, arthritis and osteoarthritis in radiographic findings, pain, range of motion and thigh circumference. According to the results, FLG technique in this 16 weeks study is comparable with other restorative techniques based on the studied parameters. This technique is valuable as it has a lot of features of other restorative techniques. it is an invasive technique and needs skill of the surgeon and there is a possibility of damage to vital structures due to slightest mistake. However, in order to ensure of the results of this technique and provide clinical usage, long-term studies with more cases and evaluation is needed.
Cruciate ligament,New technique,Dog
https://www.ivj.ir/article_48608.html
https://www.ivj.ir/article_48608_490df8b95478a33a4fb28911ce35b90c.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
13
2
2017
06
22
A Cases Report Of Myiasis In Honey Bee Broods In Khozestan, Iran
112
116
FA
HESSAM
Naghibi-Rokni
MSc of Animal Physiology, Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan
rokni_hessam@yahoo.com
mehrzad
mesbah
Professor , Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
mehrmesbah@yahoo.com
mohamad hossen
razi jalali
Associate Professor , Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
mh.jalali@scu.ac.ir
S
Tabatabaei
Associate Professor, Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan
salehtabatabaei@yahoo.com
10.22055/ivj.2017.31772.1574
Abstract: <br /> Cutaneous myiasis in honey bee broods is caused by some calliphoridae family flies. This disease causes the fatal infections in late of pupae period. In this disease, pupae of honey bee are seen in open forms and their eyes are discolored. The present infection was observed in April 2015 in one of the less populated colonies with the older queen belonged to education and research station of veterinary faculty of shahid chamran university of Ahvaz. For evaluation and definitive diagnosis, the samples were transported to the laboratory and based on the clinical observations and microscopic evaluation, cutaneous myiasis was detected in broods of honey bee. Brood die occurred in margin of frames and particularly in the final frame, and eggs and larvae as well as the third age of the parasite and also the adult flies with truancy and spawning was observed in the hive. The treatment of this infection was done by eliminating and burning the infected frames of hive. However, the acceptable method for treatment has not been recommended yet. It seems that the best way is the utilization of general prevention methods, elimination and destruction of infected frame by incineration and to bury accompanied by lime application. Put the frames outdoors and use them without disinfection, increases the risk of this parasite infection.
Honey Bee,Myesis,Calliphoridae,Iran
https://www.ivj.ir/article_48610.html
https://www.ivj.ir/article_48610_345fac314d65b52af3afe7ce20e35a7f.pdf