Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
12
2
2016
07
01
Infection of broiler chickens to Ornithobacterium rhinotracheale
In Urmia city
5
10
FA
Yadollah
Asadpour
Veterinary Department
yasadpour@yahoo.com
mehdi
Rezaei
0000-0003-1178-0260
Assistant professor, Department of clinical sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, iran.
mehdi217mr@yahoo.com
Ebrahim
Rahimabadi
Researcher Follow, Department of veterinary, Agricultural and Natural Resources Rresearch Center, Guilan, Iran
rahimabadie@yahoo.com
10.22055/ivj.2016.16408
<em> Ornithobacterium rhinotracheale</em> (ORT) a bacterium is known to be associated with respiratory disease, growth retardation, mortality and decrease egg production. The aim of the present study was conducted seroprevalence, isolation, and molecular diagnosis of <em>O. rhinotracheale</em> by PCR method. In this study, 355 serum samples were collected from 20 broiler flocks. Serological results showed that 15 flocks (75%) were positive to <em>O. rhinotracheale</em> infection by ELISA. Total of 200 tracheal swabs (20 samples) were collected. The <em>O. rhinotracheale</em> was isolated from tracheal swabs of 5 flocks (25%) by culture and but with using specific primers of ORT in PCR method was detected A 784 bp fragment of the 16SrRNA gene from 8 flocks (40%). By statistical, there was no significantly different between correlation of the isolates and flocks ORT titers (P>0.05). The results of this study indicated that the prevalence of <em>Ornithobacterium rhinotracheale</em> antibodies is high in the broiler flocks in Urmia city and PCR is a suitable method for diagnosis of the <em>O. rhinotracheale</em>
Ornithobacterium Rhinotracheale,PCR,ELISA,broiler,Urmia
https://www.ivj.ir/article_16408.html
https://www.ivj.ir/article_16408_963e06b8ce27f0aacdcbfeb2653dc538.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
12
2
2016
07
01
Seroprevalence of Linguatula serrata nymph infection in sheep from west of Iran
11
18
FA
Alireza
Alborzi
, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran.
alirezaalborzi@yahoo.com
Masoud
Ghorbanpoor
, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran
ghorbanpoor2000@yahoo.com
Hossein
Hamidinejat
, Department of Pathobiology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran
hsa.hamidinejat@gmail.com
Somayeh
Chamanara
School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran
somayechamanara@gmail.com
Sepideh
Noori
School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran
noori418@yahoo.com
10.22055/ivj.2016.16409
Linguatula serrata is one of the parasitic zoonoses. The adults, larval stages of the parasite live in nasal airways of canids and visceral organs of herbivors respectively. The aim of this study was to determine the seroprevalence of L. serrata nymph infection in sheep from west of Iran by indirect enzyme-linked immunosrobent assay (ELISA). Blood samples (n=1048) were taken from sheep of two province (Ilam (n= 598) and Kermanshah (n=450)) in west of Iran ( at least five different cities of each province). The collected sera were tested by ELISA using excretory-secretory antigens of L. serrata nymph and anti IgG sheep conjugate. Data were classified according to sex, ages of the animals (1-<2, 2-<3, 3-≥4 years), area, and sheep herds with or without dogs, analyzed using statistical software (SPSS). Seroprevalence of L. serrata infection in sheep was 30.5% (317 of 1048) from west of Iran, 27.8%(125 of 450 ) from Ilam and 32.1% (192 of 598) from Kermanshah provinces. There was significant difference in prevalence among five different area of two provinces( P<0.001). Of the animal males 31.5% (165 of 523) and females 28.9% (152 of 525) were infected with L. serrata. Infection with the parasite was not significantly associated with gender of the animals (p>0.05). Seroprevalence was significantly increased with the increase of age, lower (12.1%) in 1-<2 years and higher (49.3%) in 3-≥4 years old of the animals( P<0.01). There was significant difference in prevalence among sheep herds with (39.8%) and without (23.4%) dogs (P<0.01). This is the first report in assessment of L. serrata infection in sheep by ELISA. Relatively high prevalence of L. serrata infection in the sheep is reflection of linguatolosis in dogs from the areas, it must be consider to all progamms for control and reduction of the infection in definitive and intermediate hosts.
"Sheep,Linguatulla serrata,Antigen,ELISA,Iran"
https://www.ivj.ir/article_16409.html
https://www.ivj.ir/article_16409_7adabe8db65d2884816d9d3b4f491253.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
12
2
2016
07
01
Comparison of effects of castor oil and senna for bowel preparation before abdominal radiography in dogs
19
26
FA
Reza
Avizeh
عضو هیئت علمی دانشگاه شهید چمران اهواز
avizeh@scu.ac.ir
Alireza
Ghadiri
Shahid Chamran University of Ahvaz
alighadiri@scu.ac.ir
Bahman
Mosallnejad
Shahid Chamran University of Ahvaz
bmosallanejad@scu.ac.ir
Mahdi
Pourmahdi
Shahid Chamran University of Ahvaz
pourmahdim@scu.ac.ir
Niloofar
Ahmadian
Graduated from Shahid Chamran University of Ahvaz
niloofar.ahmadian68@yahoo.com
10.22055/ivj.2016.16410
A clean bowel preparation prior to X-ray examination is essential to obtain an accurate diagnosis. This study was performed to compare the effect of senna and castor oil on the bowel preparation of dogs for abdominal radiography. Twenty adult healthy mongrel dogs from each sex ranging in age from 18 to 30 months received either a senna (20mg/kg) or a castor oil (4ml/kg) regimen through an orogastric tube the day before abdominal radiography. Only water was given ad libitum to animals during the day before radiography. Radiologist unaware of the method of bowel preparation, reviewed the standard radiographs and graded the feces and gas in the colon and rectum, fecal radio-opacity and quality of radiographs on a scale of 0 to 3 (0=inadequate to 3=excellent). The cleanliness scores of colon for the senna and castor oil groups were 2.5±0.53 and 2.3±0.48, respectively. Also the cleanliness scores of rectum for the senna and castor oil groups were 2.5±0.53 and 2.4±0.52, respectively. Both senna and castor oil significantly improves bowel cleansing score in dogs (p < 0.05). There were no significant differences between the groups with respect to colon and rectum cleansing score, fecal radio-opacity and quality of radiographs (p ≥0.05).There were no clinically differences between the two groups for any of the side effects. It is concluded that senna and castor oil are safe and effective methods of colon cleansing, well tolerated by dogs.
Bowel Preparation,Senna,Castor oil,Dog
https://www.ivj.ir/article_16410.html
https://www.ivj.ir/article_16410_a411a36e5c13ee11b7b6177b5ee0f615.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
12
2
2016
07
01
The effects of dietary 1, 25-Dihydroxycholecalciferol (Calcitriol) and root hydroalcoholic extract of Withania Coagulans on immune response and small intestinal morphology of broiler chickens
27
42
FA
Sayed Javad
Hosseini
The Excellence Center for Animal Sciences and Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, PO Box 91775-1163, Mashhad, Iran.
h.seyedjavad@yahoo.com
محمد طاهر
میرکزهی
Department of Animal Science, Higher Educational Complex of Saravan. P.O. Box: 99516-34145, Saravan, Sistan and Baluchestan, Iran.
mt_mirakzehi@yahoo.com
Hassan
Saleh
Department of Animal Science, Higher Educational Complex of Saravan. P.O. Box: 99516-34145, Sistan and Baluchestan, Saravan, Iran.
hsaleh.um@gmail.com
10.22055/ivj.2016.16411
This study was carried out to investigate the effects of 1, 25-Dihydroxycholecalciferol (1, 25 (OH)2 D3) (calcitriol) and root hydroalcoholic extract of Withania coagulans (WC) root on immune response and small intestinal morphology of broiler chickens. Treatments were arranged factorially (2×3×2) consisted of a positive control with adequate Ca and a negative control diet (Ca level reduced by 30%), three levels of WC (0, 100 and 200 mg/kg diet), and two levels of 1, 25 (OH)2 D3 (0 and 0.5 g/kg diet). Six hundred male day old Ross 308 broiler chicks were randomly distributed into 60 floor pens, 10 birds each. Each treatment was replicated 5 times (50 birds). Each diet was fed ad libitum to chicks from day one to 42 d of age. Sheep red blood cells (SRBC) test was used as measure of humoral immunity. So, at 25 and 32 days of age chicks were injected to evaluate the primary and secondary antibody response. At 37 days of age, the cutaneous basophil hypersensitivity (CBH) test was used to evaluate cell-mediated immune response. On d 21 and 42, one bird per replicate was killed to determine small intestinal morphology and lymphoid organ weights. The results of humoral immune response showed that reduction of dietary Ca level by 30% and supplementation of WC did not significantly influence total antibody levels. The CBH response showed that supplementation of WC and calcitriol had no significant effect on interdigital skin thickness. At 42 d of age, the weight of spleen significantly reduced by feeding of negative control diet. Also, supplementation of WC at 200 mg/kg significantly increased the weight of thymus at this age. It was found that supplementation of WC at 200 mg/kg resulted in shorter villus length at 42 d of age.
Calcitriol,Withania coagulans,Immune response,intestine morphology,broiler chickens
https://www.ivj.ir/article_16411.html
https://www.ivj.ir/article_16411_f369f33ba216f90fd08727cbcf4d1d43.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
12
2
2016
07
01
Investigating Effect of adding Camelia siensies powder to diet of Cyprinus carpio and its effect on immune factors
43
54
FA
Mojgan
Khodadadi
Professor
mjkhodadadi@gmail.com
Shokufeh
Ranjbar
دانشجو
shekufeh.ranjbar@gmail.com
10.22055/ivj.2016.16412
In this experiment the effects of<em> Camellia sinensis</em> as a phytobiotic on the immunity factors of the <em>Cyprinus</em> <em>carpio</em> was examined. For this purpose 360 juvenile <em>Cyprinus</em> <em>carpio</em> with the average weight of 22± 1g were selected and after being adapted to the environment the fish were stored in 12 tanks (30 fish in each tank). The <em>Camellia sinensis</em> was added to the diet in three levels 1.5% (treatment 2) 3% (treatment 3) and 5% (treatment 4) and the diet without <em>Camellia sinensis</em> was used for the control group (treatment 1). Each treatment was recurred 3 times a day. The fish were fed 3% of their weight. At the end of the 45 day treatment period, 15 fish were randomly selected from each treatment and their blood sample was taken from the caudal vein. The immunity factors including differential white blood cell count, total protein, immunoglobulin M (IgM) serum, albumin and lysozyme were analyzed and the results showed that the lysozyme activity had Significantly difference in compared with control treatment (P<0.05) and maximum amount of it was observed in 1.5 % treatment that was equal to 135.67±15.33 . Also, maximum of total Protein 4.89±0.48 and maximum of immunoglubin M 3.35±0.52 were in 1.5 % treatment (P<0.05), but albumin amount was alfected by none of measured factors in exprement (P>0.05). In general that prescribing <em>Camellia sinensis</em> at 1.5% level orally stimulates some non-specific blood and immune factors and it can be used as the immunity stimulant for the juvenile <em>Cyprinus</em> <em>carpio</em>.
Camellia sinensis,Cyprinus carpio,Immunity factor
https://www.ivj.ir/article_16412.html
https://www.ivj.ir/article_16412_97200f59e081629c32c068809ce5288d.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
12
2
2016
07
01
Comparison of hatching, deformity, survival rates, growth indicators and body composition between all-female rainbow trout’s (Oncorhynchus mykiss) through Gynogenesis by use of gamma irradiation
55
63
FA
Mahdi
ُsoltani
عضو هیأت علمی دانشگاه تهران
msoltani@ut.ac.ir
gholamreza
shahhosseini
Member of Scientific Staff
gshahhosseini@yahoo.com
Marzieh
Heidarieh
عضو هیأت علمی
heidarieh-m@yahoo.com
Ali
Taheri Mirghaed
عضو هیأت علمی
ataherimirghaed@ut.ac.ir
Ashkan
Zargar
عضو هیأت علمی
a-zargar@ut.ac.ir
10.22055/ivj.2016.16413
Gamma radiation at doses 450, 600, 750, 900 and 1050 Gry were used. Also, early thermal shock to induce diploid was used at 28°C. Hatching, deformity and survival rates were measured after developing period in different groups. Growth performance and chemical body analysis in fish were measured at the end of trail. Histological studies on the gonads were undertaken to demonstrate the success of gynogenesis induction. The highest and the lowest hatching and survival were obtained in the triploid group (p<0.05). No significant difference was observed in survival and deformity among irradiated treatments. Treatment irradiated with 450 gray showed the highest percentage of hatching (p<0.05). Growth indices showed a significance increase in gynogen groups compared to control and triploid groups (p<0.05). There was no significant difference between chemical compositions (protein, fat, ash and dry matter) among different treatments. The gonads histology demonstrated a successful in gynogenesis using gamma radiation. According to these findings it can be concluded that the use of gynogenesis by gamma ray in rainbow trout is a suitable method for increasing the production.
Trout,Gamma radiation,Gynogenesis,Growth indicators,body composition
https://www.ivj.ir/article_16413.html
https://www.ivj.ir/article_16413_3c700e56bc7ce37b72cd72a57a8cf213.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
12
2
2016
07
01
Ultrasonographic estimation of intraperitoneal fluid volume in dogs
64
71
FA
Alireza
Ghadiri
عضو هیات علمی
alighadiri@scu.ac.ir
Reza
Avizeh
Shahid Chamran University of Ahvaz
avizeh@scu.ac.ir
Mehdi
Pourmahdi Borujeni
Shahid Chamran University of Ahvaz
pourmahdim@scu.ac.ir
Hadis
Mirzabeigee
Shahid Chamran University of Ahvaz
hadis82m@yahoo.com
10.22055/ivj.2016.16414
The ability of accurately determine intraperitoneal fluid volume would be useful in both research and clinical practice. The purpose of this study was to develop a method by which intraperitoneal fluid volume in dog can be estimated, using ultrasonography. In this survey volume of intraperitoneal fluid in six adult healthy female dogs (mean weight 16.3 kg) were determined after injection of 1000 and 2000 ml of saline 0.9% solution, in their abdominal cavities. Depth of peritoneal fluid was measured in three regions, ultrasonographically. In each region of abdomen, volume was estimated based on geometric formula, using a segment of a sphere volume. In order to generalize the results of ultrasonography, point and interval estimations with 95% confidence interval and calculation error<sup>'</sup>s percent were also calculated using SPSS software. Interval estimation showed each millimeter of ascitic fluid in ultrasonography is equal to 11 to 15 ml fluid in cranial abdomen, 14 to 20 ml in mid-abdomen and 16 to 28 ml in caudal abdomen. The volume estimation with ultrasonography in caudal to the sternum, umbilical area and cranial to the pelvis were 50.9 higher, 10.4 lower and 12.8 lower than actual volumes of 1000 ml, respectively and were 56.9 higher, 5.3 lower and 50.3 lower than actual volumes of 2000 ml, respectively. In conclusion, calculation error percentage was much more in caudal to the sternum and cranial to the pelvis than in umbilical area. As a result, the best location for volume estimation of intraperitoneal fluid was in around the umbilicus.
Dog,Ultrasonography,ascites,Intraperitoneal fluid
https://www.ivj.ir/article_16414.html
https://www.ivj.ir/article_16414_54cb33966d447ce36df3e32b4cc43002.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
12
2
2016
07
01
Investigation of immersion effect of Hypophthalmichthys molitrix fillets in garlic extract on some qualitative parameters during refrigerated storage (4°C)
72
84
FA
Zahra
Ghiasvand
عضو هیات علمی دانشکده کشاورزی و منابع طبیعی دانشگاه آزاد اسلامی واحد آزادشهر
zaghiasvand@yahoo.com
راضیه
میهن
دانش آموخته دانشکده کشاورزی و منابع طبیعی دانشگاه آزاد اسلامی واحد آزادشهر
raziehmihan@yahoo.com
فریبرز
قجقی
عضو هیات علمی دانشگاه آزاد اسلامی واحد آزادشهر
fariborzghojoghi@yahoo.com
reza
changizi
professor assistant of Islamic Azad university of babol
rech76ir@yahoo.com
10.22055/ivj.2016.16415
In order to determine the effect of garlic extract on some freshness parameters of silver carp filet, twenty numbers of silver carp were bought and after preparation of fillet with average weight (100±10 g) the experiment in laboratory was conducted. The statistical method containing 4 treatments which had various concentration of garlic extract including control (0), 0.5, 1 and 1.5%. Each treatment was performed in tree replications. Garlic extract was dissolved in distilled water then, the prepared fillets were deep bathed and immersed in experimental solutions for 10 minutes. All of the samples (fillets) were packed in polyethylene bags and were stored in refrigerator at 4 ± 1 °C for 21 days. The fillets were measured at every 7 days during storage period in equipped laboratory to determine the overall quality of fish fillets for 21 days. The results of this study clearly confirmed the role of garlic extract to improve the microbial and biochemical characteristics of silver carp fillet during the experiment. Based on analysis of total viable bacteria counts, total psychotropic bacteria counts, peroxide value, thiobarbituric acid reactive substances, total volatile basic nitrogen, pH and free fatty acids, best treatment which improved these parameters was seen in 1.5% garlic extract. Overall it can be proposed that the 1.5% garlic extract can preserve the quality of refrigerated silver carp fillet in a 14 days period.
Silver carp,Qualitative indicators,garlic extract,Storage
https://www.ivj.ir/article_16415.html
https://www.ivj.ir/article_16415_cc5ce8e39348e911f408696e800a9322.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
12
2
2016
07
01
Histological and Histometrical Study of Barbus grypus Gill
85
93
FA
Hassan
Morovvati
0000-0003-0275-1636
عضو هیات علمی
hmorovvati@scu.ac.ir
Mahmood
khaksary Mahabad
Shahid Chamran University
mkhaksary@scu.ac.ir
Ameneh
Arefi
University of Tehran
arefi_ameneh@yahoo.com
10.22055/ivj.2016.16416
In this study, a total number of 10 fish freshly prepared <em>Barbus grypus</em> and samples from proximal, middle and distal portions of gill arches were dissected. The 5-6 µm sections were made using paraffin embedding techniques and stained by haematoxylin & eosin (H&E) and periodic acid shiff (PAS). The results showed<em> Barbus grypus</em> gill has consisted from 4 gill arches. Gills are located in gill compartment on fish head lateral regions and covered by gill operculum. There are gillrakers on the cranial part and there is a holobranch on the caudal part of the each gill arch. A gill arch has consisted from primary lamellae that secondary lamellae diverge from them at one side and gill rakers at other side. Primary lamellae were covered by stratified squamous epithelium. As secondary lamellae are diverged from their axis, the primary lamellae are interrupted. There are undifferentiated cells, squamous to cuboid-shaped epithelial cells, chloride and mucous cells layer in the primary lamellae epithelium. Secondary lamellae have a vascular center containing pillar cells. Secondary lamellae epithelium is very thin and consisted of 1 or 2 cell layer which including undifferentiated and pavement cells. Gill raker has a stratified epithelium which including a lot of mucous cells. Their central part is supported by bone. Histometrical results showed that primary lamellae epithelial thickness is different from secondary lamellae epithelial thickness significantly (P≤0.05). There is a significant difference between primary lamellae in proximal portion with distal and middle portions of the right gill. The middle portions of the right primary lamellae are different with whole parts of right and left sides of the primary lamellae. There was no significant difference between secondary lamellae epithelial thickness. In conclusion, histological structure of <em>Barbus grypus</em> gill is similar to other teleostei and has no difference with Cyprinidea family.
Barbus grypus,Gill,histology,Histometry
https://www.ivj.ir/article_16416.html
https://www.ivj.ir/article_16416_3f00758f2b77980a35324a1d0c01d510.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
12
2
2016
07
01
Comparative evaluation of Garlic and Atorvastatin effects on lipid profile changes in dog
94
102
FA
Bahman
Mosallanejad
Associate
bmosallanejad@scu.ac.ir
Reza
Avizeh
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
avizeh@scu.ac.ir
محمد
راضی جلالی
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
m_jalali@scu.ac.ir
آرشام
جهانمردی
دانشجوی دانشکده دامپزشکی دانشگاه شهید چمران اهواز، اهواز، ایران.
arsham.4881@gmail.com
10.22055/ivj.2016.16417
The purpose of the present study was to evaluate the effects of garlic and atorvastatin on lipid profile changes in dogs and the influence of time on treatment process. For this purpose, 15 male dogs, 1- 2 years old were randomly divided into 3 groups with 5 animals in each group. Group A (control) included five dogs were fed with cholesterol powder (4 g/kg for 10 days) to induce hyperlipidemia. Group B was similar to group A, but in addition cholesterol, atorvastatin (5 mg/kg) was administered for 45 days. Group C was similar to group B, with the difference that garlic (100 mg/kg body) was administered instead of atorvastatin. Blood samples were collected four times during zero, 10, 40 and 55 days after start of study. Serum total cholesterol, triglycerides, HDL-C and LDL-C were measured by using commercial kits. Atorvastatin (p<0.001) and garlic (p<0.01) showed an effective hypolipidemic activity in reducing blood triglyceride. In comparison between two drugs and their effects on triglyceride, atorvastatin showed a significant difference with garlic (p<0.01). Concerning the effects of drugs on cholesterol level, a significant lowering was seen in the group receiving atorvastatin than garlic on day 40 (p<0.05). The treated groups (B and C), had good results in reduction of LDL-C, on day 55 (p<0.001). In comparison between two drug and their effects on HDL-C, a significant difference was seen between groups A and B, on day 55 (p<0.01). The present study showed that both atorvastatin and garlic have hypolipidemic activity in dogs, but atorvastatin was more effective than garlic.
Atorvastatin,Garlic,Triglyceride,Cholesterol,Dog
https://www.ivj.ir/article_16417.html
https://www.ivj.ir/article_16417_edbe6379d2c62151ed20b28f27125d11.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
12
2
2016
07
01
Invitro effect of juglans regia li. stem bark hydroalcoholic extract on some bacterial pathogens of fish
103
108
FA
نغمه
موری بختیاری
دانشگاه شهید چمران
n.moori@scu.ac.ir
zahra
Tulabi dezfuli
bGraguated student of veterinary faculty, shahid chamran university of Ahvaz, IRAN
zahratulaby@yahoo.com
Bahare
Soleimani
2دانشآموخته دکترای حرفهای دانشکده دامپزشکی دانشگاه شهید چمران اهواز، ایران
b_s_91@yahoo.com
t
m
tak.mohammadian@gmail.com
10.22055/ivj.2016.16418
Fish farmers are losing millions of dollars annually due to the large scale mortality of fish due to diseases caused by antibiotic resistance pathogens. Plant secondary metabolites show activity in the micro to submicromolar range to Gram-positive species. Plant extracts decrease the selective pressure for developing antibiotic resistance. Hence, in the present study antibacterial effect of hydroalcoholic extract of <em>Juglans regia</em> <em>Li</em>. compound against <em>Aeromonas hydrophila</em>, <em>Yersinia ruckeri</em>, <em>Streptococcus iniae</em>, <em>Staphylococcus aureus</em> and <em>Salmonella typhimurium</em> were screened. By standard protocol, hydroalcoholic extract of <em>Juglanse regia Li</em>. stem bark in pharmacology department of Shahid Chamran Veterinary School was prepared. To determine the positive control for each bacterial, antibiogram was done. In vitro evaluation of antibacterial effect of extract was done by disc diffusion method (Kirby bauer) and minimum inhibitory concentration was determind by E-test and macrodilution method. According to result in this study, clear inhibition zone compared with the positive control was seen about <em>A.hydrophila </em>and <em>Y.ruckerii</em>. But no strong effect on other studied bacteria was observed. By E-test, minimum inhibitory concentration of extract in <em>Y.ruckerii</em> and<em> A.hydrophila </em>was evaluated,3.9 and 12 mg/ml,respectively. Minimum inhibitory concentration of extract in <em>Y.ruckerii</em> and<em> A.hydrophila</em> by macrodilution was 3.9 and 62.5 mg/ml and minimum bactericidal concentration was 7.81 and 125 mg/ml, respectively.<em> Y.ruckerii</em> and <em>A.hydrophila</em> are important bacteria in the aquaculture industry and hydroalcoholic extract of this plant was effective on them but more work should be done on its safety and toxicity. Other studied bacteria which extract had any effect on them, should be examined with other form (alcoholic or aqueous) of stem bark <em>Juglans regia Li</em>. extract because type of solvent or the method of preparation can affect antibacterial activity of extract.
juglans regia li,hydroalcoholic extract,fish,bacterial pathogens
https://www.ivj.ir/article_16418.html
https://www.ivj.ir/article_16418_aa9d3256d152c437e9cda43be83dedd9.pdf
Shahid Chamran University of Ahvaz
Iranian Veterinary Journal
1735-6873
2676-704X
12
2
2016
07
01
Case report of a dicephalus fetus with ectopic liver in cow
109
114
FA
Kaveh
Khazaeel
0000-0002-4505-1106
عضو هیات علمی دانشگاه شهید چمران اهواز
k.khazaeil@scu.ac.ir
یزدان
مظاهری
عضو هیات علمی دانشگاه شهید چمران اهواز
ymazaheri@yahoo.com
رضا
رنجبر
عضو هیات علمی دانشگاه شهید چمران اهواز
dabir120@yahoo.com
محمود
خاکساری مهابادی
عضو هیات علمی دانشگاه شهید چمران اهواز
mkhaksary@scu.ac.ir
جمال
نوری نزاد
عضو هیات علمی دانشگاه شهید چمران اهواز
jnourinezhad@gmail.com
علی رضا
غدیری
عضو هیات علمی دانشگاه شهید چمران اهواز
alighadiri@scu.ac.ir
10.22055/ivj.2016.16419
One apparently female dicephalus fetus of a hybrid Holstein cow have been carried to<em> </em>division of anatomy & embryology, faculty of Veterinary Medicine, Shahid Chamran University from Ahvaz slaughter house. Fetus age was estimated about 5.5 month, based on CRL = 39 cm for left fetus and CRL = 37cm for right ones. Some radiographs were prepared before dissection which revealed separated cervical and thoracic vertebra and a common lumbar vertebra. Two unequal hearts in a common pericardium, one pair lungs, one cranial vena cava, two caudal vena cava, two esophagus and two aorta which were blended each other and were made thoracic aorta, in thorax dissection were seen. Right liver was normal and was located in the abdominal cavity, but left ones was located in the thoracic cavity and were diagnosed ectopic liver. There are several reports about dicephalus fetuses in cows and other domestic animals, but it seems this case is the first report of this kind of malformation with ectopic liver in Iran.
Dicephalus fetus,Cow,Ectopic liver
https://www.ivj.ir/article_16419.html
https://www.ivj.ir/article_16419_f774b5d149945b52579b2e18193c253a.pdf