ORIGINAL_ARTICLE
Molecular detection and phylogenetic analysis of Peste des petits ruminant’s virus in QOM Province 2016 and 2018
AbstractBackground: Peste des Petits Ruminants (PPR) is a severe, highly infectious and fatal viral disease of small ruminants. Four lineages of PPR virus have been identified globally based on sequence analysis nucleoprotein (N) and fusion (F) gene. The aim of this study was molecular detection and phylogenetic analysis recently circulating PPR virus in small ruminants in QOM Province in Iran. A total of 10 anti-mortem samples (mesenteric lymph node) were collected from clinically suspicious animals and examined for the presence of PPRV by a one-step RT-PCR assay. Samples positive with RT-PCR were subjected to subsequently genetically characterized by sequencing of the nucleoprotein (N) gene and phylogenetic analysis of PPR virus (PPRV) strains.Results: Of the 10 clinical samples examined, 40.0% were positive with RT PCR for viral nucleic acid. The nucleotide sequence and phylogenetic analysis indicated that these isolates were clustered genetically with Lineage IV isolates of the PPRV.Conclusion and discussion: phylogenetic analysis and molecular findings of this study confirmed active lineage IV PPRV infections among goat populations in QOM Province.This study and latter studies revealed that the Iranian PPRV isolates belong to lineage IV and are closely related to the Pakistan, Tajikistan and India isolates of PRPV. We recommended that more studies should be done from other parts of the country, especially the border areas, in order to more accurately illustrate the status and circulation of the active lineage of the PPR virus in Iran.
https://www.ivj.ir/article_128889_7fc996b0ed54d327e7865bbc10ecec99.pdf
2021-02-19
5
15
10.22055/ivj.2019.192722.2162
Peste des Petits Ruminants (PPR)
Paramyxovirus
Molecular Detection
lineage
Hassan
Bazyar
bazyar.h@gmail.com
1
DVSc Graduated in Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ahvaz, Iran
LEAD_AUTHOR
Mohammad
Nouri
mnoori25@gmail.com
2
Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
Ali-Reza
Ghadrdan-Mashhadi
kianeg2000@yahoo.com
3
Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
Masoud Reza
Seyfi Abad Shapouri
masoudrs@yahoo.com
4
Professor, Department of pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
Hamidreza
Varshovi
hr_varshovi@yahoo.com
5
Assistant Professor, Department of Animal Viral Vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
AUTHOR
William G
DUNDON
w.dundon@iaea.org
6
Consultant Molecular Microbiologist, Joint FAO Division of Nuclear Techniques in Food and Agriculture, IAEA Laboratories Seibersdorf, Vienna, Austria
AUTHOR
Zeinab
Hedayati
hedayaty2000@yahoo.com
7
Assistant Professor, Department of Animal Viral Vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
AUTHOR
Abdollahpour, G., Raoofi, A., Najafi, J., Sasani, F., & Sakhaie, E. (2006). Clinical and para-clinical findings of a recent outbreaks of peste des petits ruminants in Iran. Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health, 53(Suppl 1): 14–16.
1
Albina, E., Kwiatek, O., Minet, C., Lancelot, R., de Almeida, R. S., & Libeau, G. (2013). Peste des petits ruminants, the next eradicated animal disease. Veterinary Microbiology, 165: 38–44.
2
Ashley, C., & Banyard, Satya Parida. (2014). Peste des Petits Ruminants Virus Molecular Epidemiology of Peste des Petits Ruminants Virus Chapter, 5 (261): 69-95.
3
Taylor, W. P., & Barrett, T. (2007). Rinderpest and peste des petits ruminants. Diseases of Sheep, 61, 450-469.
4
Bailey, D., Banyard, A. C., Dash, P., Ozkul, A., & Barrett, T. (2005). Full genome sequence of peste des petits ruminants virus, a member of the morbillivirus genus. Virus Research, 110: 119-24.
5
Bazarghani, T. T., Charkhkar, S., Doroudi, J., & Bani Hassan, E. (2006). A review on peste des petits ruminants (PPR) with special reference to PPR in Iran. Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health, 53: 17–18.
6
Couacy-Hymann, E. (2015). Current Advances in Genome Detection of Peste Des Petits Ruminants Virus. Peste Des Petits Ruminants Virus. Springer, 155–69.
7
Esmaelizad, M., Jelokhani-Niaraki, S., & Kargar-Moakhar, R. (2011). Phylogenetic analysis of peste des petits ruminants virus (PPRV) isolated in Iran based on partial sequence data from the fusion (F) protein gene. Turkish Jornal of Biology, 35: 45–50.
8
Furley, C. W., Taylor, W. P., & Obi, T. U. (1987). An outbreak of peste des petits ruminants in a zoological collection. Veterinary Record, 121: 443-447.
9
Gibbs, E. P., Taylor, W. P., Lawman, M. J., & Bryant, J. (1979). Classification of peste des petits ruminants virus as the fourth member of the genus morbillivirus. Intervirology, 11: 268–74.
10
Kerur, N., Jhala, M. K. & Joshi, C. G. (2008). Genetic characterization of Indian peste des petits ruminants virus (PPRV) by sequencing and phylogenetic analysis of fusion protein and nucleoprotein gene segments. Research in Veterinary Science, 85: 176–83.
11
Kwiatek, O., Minet, C., Grillet, C., Hurard, C., Carlsson, E., Karimov, B., et al. (2007). Peste des petits ruminants (PPR) outbreak in Tajikistan. Journal of Comparative Pathology, 136(2-3): 111-119.
12
Megersa, B., Biffa, D., Belina, T., Debela, E., Regassa, A., Abunna, F., et al. (2011). Serological investigation of Peste des Petits ruminants (PPR) in small ruminants managed under pastoral and agro-pastoral systems in Ethiopia. Small Ruminant Research, 97: 134–8.
13
Munir, M., Zohari, S., & Berg, M. (2013). Molecular biology and pathogenesis of Peste des Petitis ruminants. Berlin: Springer p: 1-152.
14
Munir, M., Zohari, S., Saeed, A., Khan, Q. M., Abubakar, M., LeBlanc, N., & Berg, M. (2012b) Detection and phylogenetic analysis of peste des petits ruminants virus isolated from outbreaks in Punjab, Pakistan. Transboundary and Emerging Diseases, 59 (1): 85-93.
15
Radostits, O. M., Gay, C. C., Hinchcliff, K. W., & Constable, P. D. (Eds.). (2006). Veterinary Medicine E-Book: A textbook of the diseases of cattle, horses, sheep, pigs and goats. Elsevier Health Sciences. Saunders Ltd. pp:730-760
16
Shaila, M. S., Shamaki, D., Forsyth, M. A., Diallo, A., Goatley, L., Kitching, R. P., & Barrett, T. (1996). Geographic distribution and epidemiology of peste des petits ruminants virus. Virus Research, 43(2): 149-53.
17
Shahriari, R., Khodakaram-Tafti, A., & Mohammadi, A. (2019). Molecular characterization of Peste des Petits ruminants virus isolated from four outbreaks occurred in southern Iran. BMC Veterinary Research, 15: 177.
18
Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). Molecular Evolution Genetics Analysis version MEGA 6 Molecular Biology and Evolution, 30: 2725-2729.
19
Zaher, K. S., & Ahmed, W. M. (2014). Isolation and identification of field isolate of Peste des Petits Ruminants Virus in Egypt. Global Veterinaria, 12: 667-672.
20
ORIGINAL_ARTICLE
Study of the presence of blaTEM, blaSHV and blaCTX-M genes in Escherichia coli strains isolated from sheep in Kerman province
The aim of this study was to the determination of prevalence of resistant Escherichia coli isolates to commonly used β-lactam antibiotics and some related resistance genes in sheep. Totally, 67 E. coli isolates from 67 healthy sheep were considered to determine resistance against 9 antibiotics belonging to commonly used beta-lactam antibiotics by disc diffusion method. Also, the presence of blaTEM, blaSHV and blaCTX-M genes was investigated by PCR. The results showed all isolates were resistant to at least one of the tested antibiotics. The high prevalence of resistant strains to cephalexin, cefotaxime and ceftazidime was 98.5%, 98.5% and 97%, respectively. Also, 5 samples (7.4%) were positive for ESBLs producing E. coli. The results of this study indicated an increasing rate of resistance to commonly used β-lactam antibiotics among sheep. Therefore, antibiotic prescription methods should be limited and prevention strategies should be considered against infections to avoid dissemination of antibiotic resistance in food-producing animals.
https://www.ivj.ir/article_128890_1e8ec5e587beadda96cc75aa0f75bf0a.pdf
2021-02-19
16
23
10.22055/ivj.2019.190457.2154
β-Lactam
Antibiotic Resistance
Escherichia coli
ESBLs
Maziar
Jajarmi
maziar.jajarmi@uk.ac.ir
1
Assisstant Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
AUTHOR
Amir
Asadabadi Safat
amir_asadabadi@yahoo.com
2
DVM Graduated, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
LEAD_AUTHOR
Ehsanollah
Sakhaee
ehsan_sakhaee@yahoo.com
3
Professor, Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
AUTHOR
reza
ghanbarpour
ghanbar@uk.ac.ir
4
Professor, Molecular Microbiology Research Group, Shahid Bahonar University of Kerman, Kerman, Iran
AUTHOR
Adefarakan, T. A., Oluduro, A. O., David, O. M., Ajayi, A. O., Ariyo, A. B., & Fashina, C. D. (2014). Prevalence of antibiotic resistance and molecular characterization of Escherichia coli from faeces of apparently healthy rams and goats in Ile-Ife, Southwest, Nigeria. Ife Journal of Science, 16(3), 447-460.
1
Aliasadi, S., & Dastmalchi Saei, H. (2015). Fecal carriage of Escherichia coli harboring extended-spectrum beta-lactamase (ESBL) genes by sheep and broilers in Urmia region, Iran. Iranian Journal of Veterinary Medicine, 9(2), 93-101.
2
Balami, E. Y., Abdulrahman, H. I., Gashua, M. M., Galadima, H. B., & Gulani, I. A. (2018). Occurrence of Multi Drug Resistant Commensal Escherichia coli in Apparently Healthy Lambs and Kids from Maiduguri, Northeastern Nigeria. Asian Journal of Research in Animal and Veterinary Sciences, 1-10.
3
Carattoli, A. (2008). Animal reservoirs for extended spectrum β‐lactamase producers. Clinical Microbiology and Infection, 14, 117-123.
4
Erb, A., Stürmer, T., Marre, R., & Brenner, H. (2007). Prevalence of antibiotic resistance in Escherichia coli: overview of geographical, temporal, and methodological variations. European Journal of Clinical Microbiology & Infectious Diseases, 26(2), 83-90.
5
Geser, N., Stephan, R., & Hächler, H. (2012). Occurrence and characteristics of extended-spectrum β-lactamase (ESBL) producing Enterobacteriaceae in food producing animals, minced meat and raw milk. BMC Veterinary Research, 8(1), 1-9.
6
Kapoor, G., Saigal, S., & Elongavan, A. (2017). Action and resistance mechanisms of antibiotics: A guide for clinicians. Journal of Anaesthesiology, Clinical Pharmacology, 33(3), 300.
7
Marshall, B. M., & Levy, S. B. (2011). Food animals and antimicrobials: impacts on human health. Clinical Microbiology Reviews, 24(4), 718-733.
8
Mølbak, K. (2004). Spread of resistant bacteria and resistance genes from animals to humans–the public health consequences. Journal of Veterinary Medicine, Series B, 51(8‐9), 364-369.
9
WHO. (2014). Antimicrobial resistance global report on surveillance: 2014 summary (No. WHO/HSE/PED/AIP/2014.2). World Health Organization.
10
Pehlivanoglu, F., Turutoglu, H., Ozturk, D., & Yardimci, H. (2016). Molecular characterization of ESBL-producing Escherichia coli isolated from healthy cattle and sheep. Acta Veterinaria, 66(4), 520-533.
11
Pitout, J. (2012). Extraintestinal pathogenic Escherichia coli: a combination of virulence with antibiotic resistance. Frontiers in Microbiology, 3, 9.
12
Ramos, S., Igrejas, G., Silva, N., Jones-Dias, D., Capelo-Martinez, J. L., Caniça, M., & Poeta, P. (2013). First report of CTX-M producing Escherichia coli, including the new ST2526, isolated from beef cattle and sheep in Portugal. Food Control, 31(1), 208-210.
13
Roschanski, N., Fischer, J., Guerra, B., & Roesler, U. (2014). Development of a multiplex real-time PCR for the rapid detection of the predominant beta-lactamase genes CTX-M, SHV, TEM and CIT-type AmpCs in Enterobacteriaceae. PloS One, 9(7), e100956.
14
Tacconelli, E., Pezzani, M. (2019). Public health burden of antimicrobial resistance in Europe. The Lancet Infectious Diseases, 19(1):4-6.
15
Tenover, F. C. (2006). Mechanisms of antimicrobial resistance in bacteria. The American Journal of Medicine, 119(6), 3-10.
16
CLSI. (2018). Performance standards for antimicrobial susceptibility testing. Clinical and Laboratory Standards Institute supplement M100.
17
ORIGINAL_ARTICLE
A study on the effect of chromium sources on thyroid hormones and blood parameters in broiler chicks under physiological stress
In poultry farms, the stresses are inevitable, therefore, it is very necessary to achieve an efficient and practical way to control and reduce its negatives effects. Thus, this study was conducted to evaluate the effects of different sources of chromium on thyroid hormones and some blood parameters of broiler chicks under physiological stress conditions. A total of 216 one day old Ross broiler chicks (male) were used in a 3 × 2 factorial arrangement with two stress conditions (no stress, stress) and three levels of additive (no Additive, 2000ppb chromium-methionine, and 2000ppb chrome-methionine mill) in a completely randomized design with 6 treatments, 3 replicates and 12 bird per each. Dexamethasone was added to the diet (1.5 mg/kg diet) during 24-18 days of age to induce physiological stress. The birds were fed with chromium diets from 18 d until the end of the experiment. Blood samples were taken at 24 and 46 days of age via brachial vein and then analyzed for blood glucose, lipids, cortisol, thyroxine (T4) and thyroid iodothyronine (T3). Physiological stress reduced the concentration of thyroid hormones, glucose, serum lipids and cortisol hormones. In stressed birds, the use of chrome methionine diet significantly reduced cholesterol of serum. Chromium supplementing diet increased thyroxine (T4) concentration. Dietary chromium methionine significantly decreased cortisol concentrations of serum. Based on the results, adding chromium ppb 2000 from various sources reduces the effects of physiological stress on thyroid hormones and cholesterol concentrations in the serum of birds under physiological stress.
https://www.ivj.ir/article_128893_7fcb3b4cbf4874d1740617d0a5e20e51.pdf
2021-02-19
24
34
10.22055/ivj.2019.159626.2095
Physiological stress
Thyroid hormones
blood parameters
Chromium
broiler chickens
Seyedeh Khoshghadam
Hoseini
seyedeh.hoseini.k@ut.ac.ir
1
MSc Graduated, College of Aburaihan, University of Tehran. Tehran, Iran
AUTHOR
Seyed Davood
Sharifi
sdsharifi@ut.ac.ir
2
Associate Professor, Department of Animal and Poultry, College of Aburaihan, University of Tehran, Tehran, Iran
LEAD_AUTHOR
Maryam
Bagheri Varzaneh
bagheri_m58@yahoo.com
3
Assistant Professor, Scientific and Industrial Research Organization, Tehran, Iran
AUTHOR
shekofeh
Ghazanfari
sharifi_sd@yahoo.com
4
Associate Professor, Department of Animal and Poultry, College of Aburaihan, University of Tehran, Tehran, Iran
AUTHOR
Aengwanich, W., & Chinrasri, O. (2003). Effect of dexamethasone on differential white blood cell counts and heterophil/lymphocyte ratio in Japa-nese quails (Coturnix coturnix japonica). Song-klanakarin Journal of Science and Technology, 25: 183-189.
1
Anderson R. A., & Kozlovsky A. S. (1985). Chromium intake, absorption and excretion of subjects consuming self-selected diets. The American Journal of Clinical Nutrition, 41: 1177-1183.
2
Barzegar Yarmohammadi A., Sharifi S. D., & Mohammadi-Sangcheshmeh A. (2020). Efficacy of dietary supplementation of nanoparticles-chromium, chromiummethionine and zinc-proteinate, on performance of Japanese quail under physiological stress. Italian Journal Of Animal Science, 19: 1123-1134.
3
Bernjian, A., Sharifi, S. D., Mohammadi Sang Cheshmeh, A., & Ghazanfari, S. (2018). Effect of chromium nanoparticles on physiological stress induced by exogenous dexamethasone in japanese quails. Biologycal Trace Element Researh, 184: 474–481.
4
Cole, T., Wilcox, H., & Heimberg, M. (1982). Effects of adrenalectomy and dexamethasone on hepatic lipid metabolism. Journal of Lipid Research, 23: 81-91.
5
Foucaud, L., Niot, I., Kanda, T., & Besnard, P. (1998). Indirect dexamethasone down-regulation of the liver fatty acid-binding protein expression in rat liver. Biochimistry Biophysic Acta (BBA)-Lipids Lipid Metabolism, 1391(2):204–212.
6
Ebrahimnazhad, Y., & Ghanbari, S. (2014). The effect of dietary chromi-um supplementation on blood biochemical parameters of broilerchicks. Greener Journal of Biollogycal Scieince, 4:098–102.
7
Gao, J., Lin, H., Song, Z., & Jiao, H. (2008). Corticosterone alters meat quality by changing pre-and postslaughter muscle metabolism. Poultry Science, 87: 1609-1617.
8
Hassanzadeh, M., Moghimi Niaki, A. A., Babapour, V., Mohit, A., & Mirzaie, S. (2016). A study of the employment of melatonin supplementation and darkness regime on reducing the negative effects of acute heat stress and mortality in broiler chickens. Iranian Journal of Veterinary Medicine, 10: 7-17.
9
Hu, X., Guo, Y., Huang, B., Zhang, L., Bun, S., Liu, D. et al. (2010). Effect of corticosterone administration on small intestinal weight and expression of small intestinal nutrient transporter mRNA of broiler chickens. Asian-Australasian Journal of Animal Sciences, 23: 175-181.
10
Jingjing, X., Li, T., Lin, L., Liyang, Z., Xi, L., Hsiao-Ching, L. et al. (2015). Effects of acute and chronic heat stress on plasma metabolites, hormones and oxidant status in restrictedly fed broiler breeders Poultry Science, 63: 66-69.
11
Lee, D. N., Wu, F. Y., Cheng, Y. H., Lin, R. S., & Wu, P. C. (2003). Effects of dietary chromium picolinate supplementation on growth performance and immune responses of broilers. Asian Australasian Journal of Animal Sciences, 16: 227-233.
12
Li, Y., Cai, H., Liu, G., Dong, X., Chang, W., Zhang, S. et al. (2009). Effects of stress simulated by dexamethasone on jejunal glucose transport in broilers. Poultry Science, 88, 330-337
13
Lien, T., Horng, Y., & Yang, K., (1999). Performance, serum characteristics, carcase traits and lipid metabolism of broilers as affected by supplement of chromium picolinate. British Poultry Science, 40, 357-363.
14
Lin, H., Sui, S., Jiao, H., Buyse, J., & Decuypere, E. (2006a). Impaired development of broiler chickens by stress mimicked by corticosterone exposure. Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology, 143, 400-405
15
Lin, H., Decuypere, E., & Buyse, J. (2006b). Acute heat stress induces oxidative stress in broiler chickens. Comparative biochemistry and physiology part a: Molecular and Integrative Physiology, 144, 11-17.
16
Mirfendereski, E., & Jahanian, R. (2015). Effects of dietary organic chromium and vitamin C supplementation on performance, immuneresponses, blood metabolites, and stress status of laying hens subjected to high stocking density. Poultry Science, 94, 281–288.
17
Moeini, M. M., Bahrami, A., Ghazi, S., & Targhibi, M. R. (2011). The effect of different levels of organic and inorganic chromium supplementation on production performance, carcass traits and some blood parameters of broiler chicken under heat stress condition. Biological Trace Element Research, 144, 715-724.
18
Odihambo Mamma, J., Thaxton, J., Vizzier-Thaxton, Y. & Dodson, W. (2006). Physiological stress in laying hens. Poultry Science, 85: 761-769.
19
Ognik, K., & Sembratowicz, I. (2012). Stress as a factor modifying the metabolism in poultry. a review. Annals universities marine curie-skłodowska. Section eye: Zootechnica, 30.
20
Pechova, A., & Pavlata, L. (2007). Chromium as an essential nutrient: A review. Veterinarni Medicina-Praha, 52: 1-6.
21
Puvadolpirod, S., & Thaxton, J. (2000). Model of physiological stress in chickens 1. Response parameters. Poultry Science, 79: 363-369.
22
Sahin, K., Sahin, N., Onderci, M., Gursu, F., & Cikim, G. (2002). Optimal dietary concentration of chromium for alleviating the effect of heat stress on growth, carcass qualities, and some serum metabolites of broiler chickens. Biological Trace Element Research, 89: 53-64.
23
Sirirat, N., Lug, J. J., Hung, A. T. Y., Chin, S. Y., & Lien, T. F. (2012). Effects different levels of nanoparticles chromium picolinate supplementation on growth performance, mineral retention, and immune responses in broiler chickens. Journal of Agricultural Science, 4, 48-58.
24
Smith, M., & Teeter, R. (1987). Potassium balance of the 5 to 8-week-old broiler exposed to constant heat or cycling high temperature stress and the effects of supplemental potassium chloride on body weight gain and feed efficiency. Poultry Science, 66, 487-492.
25
Zhao, L., Zeng, J., Sun, S., Ding, H., Loud, H., & Li, W. (2009). Chromium (iii) nanoparticles affect hormone and immune responses in heat-stressed rats. Biological trace element research, 129, 157-169.
26
Taha, N. M, Mandour A. A., & Habeila, O. H. (2013). Biochemical effect ofchromium element on lipid profile of broilers. Alex Journal of Veterinary Science, 39, 74–81
27
Toghyani, M., Toghyani, M., Shivazad, M., Gheisari, A. and Bahadoran, R. (.2012). Chromium supplementation can alleviate the negative effects of heat stress on growth performance, carcass traits, and meat lipid oxidation of broiler chicks without any adverse impacts on blood constituents. Biological trace element research, 146, 171-180.
28
Virden, W. & Kidd, M. (2009). Physiological stress in broilers: Ramifications on nutrient digestibility and responses. Journal of applied poultry research, 18: 338-347.
29
Vincent, J. B. (2001). The bioinorganic chemistry of chromium (III). Polyhedron, 20(1),1–26.
30
ORIGINAL_ARTICLE
Effects of sperm parameters and incubation conditions of sperms with foreign DNA in ovine sperm transfection
Sperm-mediated gene transfer (SMGT) based on the intrinsic ability of sperms to bind and take-up of exogenous DNA was introduced in 1989. From that time on, it has been a challenging topic. One of the serious challenges was the motility of transfected sperms and hence their ability to fertilize the oocyte. The present study aimed to determine the effects of media and incubation conditions and also sperm parameters on the transfection of ovine spermatozoa by foreign DNA. In this study, the effects of various incubation temperature (5, 20 and 37 ºC), three different media (PBS, TCM and DMEM), presence of FBS in medium, viability and motility of sperms and sperm capacitation in DNA absorption rate and intensity were assessed by using rhodamine-labeled EGFP plasmid. Results showed that incubation of spermatozoa with plasmid in 37ºC leads to more transfection rate but various incubation media and presence or absence of FBS had no significant effect on DNA uptake rate and intensity. Motility rate and capacitation of sperms had no significant effects too. However, in sperms with a damaged membrane, the DNA uptake rate increased significantly. All of the spermatozoa with true DNA absorption (post acrosome absorption) were immotile and none of the examined treatments in this study could produce motile transfected spermatozoa. Regarding the results of this study, it seems that membrane-damaged spermatozoa incubated with foreign DNA can be used for SMGT-ICSI to produce transgenic animals.
https://www.ivj.ir/article_128894_61d5c6ee44ea42b7a8072ed677463215.pdf
2021-02-19
35
46
10.22055/ivj.2019.184521.2134
Ovine
SMGT
Sperm
Transfection
Khosro
Hoseini Pajooh
pajhooh@yahoo.com
1
Assistant Professor, Department of Biotechnology, Iranian Research Organization for Science and Technology, Tehran-Iran
LEAD_AUTHOR
Parviz
Tajik
p.tajik@ut.ac.ir
2
Professor, Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
Anzar, M., & Buhr, M. (2006). Spontaneous uptake of exogenous DNA by bull spermatozoa. Theriogenology, 65(4), 683-690.
1
Arias, M. E., Sanchez-Villalba, E., Delgado, A., & Felmer, R. (2017). Effect of transfection and co-incubation of bovine sperm with exogenous DNA on sperm quality and functional parameters for its use in sperm-mediated gene transfer. Zygote, 25(1), 85-97. doi:10.1017/s096719941600037x
2
Atkinson, P. W., Hines, E. R., Beaton, S., Matthaei, K. I., Reed, K. C., & Bradley, M. P. (1991). Association of exogenous DNA with cattle and insect spermatozoa in vitro. Molecular Reproduction and Development, 29(1), 1-5.
3
Blash, S., Melican, D., & Gavin, W. (2000). Cryopreservation of epididymal sperm obtained at necropsy from goats. Theriogenology, 54(6), 899-905.
4
Buhr, M. M., Canvin, A. T., & Bailey, J. L. (1989). Effects of semen preservation on boar spermatozoa head membranes. Gamete Research, 23(4), 441-449. doi:10.1002/mrd.1120230409
5
Cabrera, M., Chan, P., Kalugdan, T., & King, A. (1997). Transfection of the inner cell mass and lack of a unique DNA sequence affecting the uptake of exogenous DNA by sperm as shown by dideoxy sequencing analogues. Journal of Assisted Reproduction and Genetics, 14(2), 120-124. doi:10.1007/bf02765781
6
Camaioni, A., Russo, M., Odorisio, T., Gandolfi, F., Fazio, V., & Siracusa, G. (1992). Uptake of exogenous DNA by mammalian spermatozoa: specific localization of DNA on sperm heads. Reproduction, 96(1), 203-212.
7
Canovas, S., Gutierrez-Adan, A., & Gadea, J. (2010). Effect of exogenous DNA on bovine sperm functionality using the sperm mediated gene transfer (SMGT) technique. Molecular Reproduction and Development, 77(8), 687-698. doi:10.1002/mrd.21205
8
Eghbalsaied, S., Ghaedi, K., Hosseini, S., Tanhaie, S., Forouzanfar, M., Hajian, M., . . . Nasr Esfahani, M. (2009). Selection of the Most Appropriate Medium for Assessing Motility and DNA Uptake of Bovine Spermatozoa. Yakhteh Medical Journal,, 10( 4), 266-271.
9
Eghbalsaied, S., Nasr-esfahani, M. H., Ghaedi, K., & Mozafari, N. (2008). Lipofectamine Does Not Enhance Bovine Sperm Transfection Efficiency Yakhteh Medical Journal, 10(1),48
10
Gandolfi, F. (1998). Spermatozoa, DNA binding and transgenic animals. Transgenic Res, 7(3), 147-155.
11
García-Vázquez, F., Gutiérrez-Adán, A., & Gadea, J. (2009). Evaluation of binding sperm-exogenous DNA in ejaculate and epididimary porcine spermatozoa. Archivos de Medicina Veterinaria, 41(2), 131-138.
12
García-Vázquez, F. A., García-Roselló, E., Gutiérrez-Adán, A., & Gadea, J. (2009). Effect of sperm treatment on efficiency of EGFP-expressing porcine embryos produced by ICSI-SMGT. Theriogenology, 72(4), 506-518.
13
Gordon, J. W., & Ruddle, F. H. (1981). Integration and stable germ line transformation of genes injected into mouse pronuclei. Science, 214, 1244-1246.
14
Hassanane, M. S., El Makawy, A. I., Helalia, S. M., Abdoon, A. S., Khalil, K. M. A., Ghanem, T. A., . . . Shen, W. (2017). First study of sperm mediated gene transfer in Egyptian river buffalo. Journal of Genetic Engineering and Biotechnology, 15(2), 475-482.
15
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Hoseini pajooh, K., Tajik, P., Karimi poor, M., & Behdani, M. (2014). Tranfection of ram spermatozoa with pEGFP carrying human lysozyme gene. Journal of Veterinary Resarch, 69(3), 245-254.
18
Hoseini Pajooh, K., Tajik, P., Karimipoor, M., & Behdani, M. (2016). Techniques for augmentation of exogenous DNA uptake by ovine spermatozoa. Iranian Journal of Veterinary Research, 17(1), 25-30.
19
Hoseini Pajooh, K., Tajik, P., & Karimipour, M. (2014). Dynamics of interaction between ram sperm with plasmid carrying human lysozyme gene in SMGT. Journal of Comparative Pathobiology, 11,(3), 1331-1344.
20
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21
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22
Lavitrano, M., Forni, M., Bacci, M. L., Di Stefano, C., Varzi, V., Wang, H., & Seren, E. (2003). Sperm mediated gene transfer in pig: Selection of donor boars and optimization of DNA uptake. Molecular Reproduction and Development, 64(3), 284-291.
23
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24
Li, L., Shen, W., Min, L., Dong, H., Sun, Y., & Pan, Q. (2006). Human lactoferrin transgenic rabbits produced efficiently using dimethylsulfoxide-sperm-mediated gene transfer. Reprod Fertil Dev, 18(6), 689-695.
25
Pereyra-Bonnet, F., Fernández-Martín, R., Olivera, R., Jarazo, J., Vichera, G., Gibbons, A., & Salamone, D. (2008). A unique method to produce transgenic embryos in ovine, porcine, feline, bovine and equine species. Reproduction, Fertility and Development, 20(7), 741-749.
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27
Perry, A. C. F., Wakayama, T., Kishikawa, H., Kasai, T., Okabe, M., Toyoda, Y., & Yanagimachi, R. (1999). Mammalian Transgenesis by Intracytoplasmic Sperm Injection. Science, 284(5417), 1180-1183. doi:10.1126/science.284.5417.1180
28
Rubessa, M., Lotti, S. N., Kandel, M. E., Popescu, G., & Wheeler, M. B. (2019). SLIM microscopy allows for visualization of DNA-containing liposomes designed for sperm-mediated gene transfer in cattle. Molecular Biology Reports, 46(1), 695-703.
29
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30
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31
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Simões, R., Binelli, M., Nicacio, A. C., Milazzotto, M. P., Feitosa, W. B., Visintin, J. A., & Assumpção, M. E. O. A. (2007). Use of spermatozoa as vectors of exogenous dna for in vitro production of bovine transgenic embryos. Reproduction, Fertility and Development, 19(1), 320-321.
34
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35
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36
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37
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38
Zhao, Y., Yu, M., Wang, L., Li, Y., Fan, J., Yang, Q., & Jin, Y. (2012). Spontaneous uptake of exogenous DNA by goat spermatozoa and selection of donor bucks for sperm-mediated gene transfer. Molecular Biology Reports., 39(3), 2659-2664.
39
ORIGINAL_ARTICLE
Genotype profiling of Mycobacterium avium subspecies paratuberculosis isolates recovered from feces and milk of Iranian bovine, ovine and caprine hosts, are the sheep (S) type strains mysteriously overlooked in Iran
Mycobacterium avium subspecies paratuberculosis (MAP) is known worldwide for its role in causing paratuberculosis (Ptb), a chronic incurable intestinal tract infection of ruminants. Concerns remain in place as the elusive MAP epidemiology and its potential zoonotic nature continue to challenge veterinarians across the world. Almost half a century since its initial reports, Ptb is still not a notifiable disease in Iran leading to the unfortunate lack of a public-funded control scheme. We undertook this study to extend our knowledge of the MAP population genetic in diverse hosts in Iran. During seven months, 35 MAP isolates collected from milk and feces obtained from sheep, goat and cattle originating 8 provinces plus a further eleven archived MAP isolates were genetically interrogated. The Collins strategy was conducted on examined isolates to differentiate between cattle and sheep types of MAP bacteria. The study was further expanded by RFLP-IS900 analysis using PstI in search for genetic diversity. The findings of Collins strategy showed that all animal species covered by the study were exclusively infected by 'cattle' strains. In genotyping, on the other hand, RFLP-IS900 analysis yielded 10 largely similar genotypes with five of them infecting multiple hosts. Nevertheless, no trace of varying profiles was detected in individual farms and conspecifics. These data, taken together with earlier observations by others, raise the question of the mysterious absence of sheep-type strains in Iran. Circulation of highly similar strains in Iranian farm ruminants might be an indication of a homogenic MAP population with a tendency for diversification started only recently. The extent of temporal and spatial characteristics of such diversification is still unclear and left for future works.
https://www.ivj.ir/article_128897_be275b601a1fdefee425c9bbb856bbad.pdf
2021-02-19
47
54
10.22055/ivj.2019.187699.2140
Paratuberculosis
Mycobacterium avium
Sheep type strain
Lida
Abdolmohamadi
mohammadimail1396@gmail.com
1
PhD Graduated of bacteriology, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
Masoud
Haghkhah
mhaghkha@shirazu.ac.ir
2
Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
Nader
Mosavari
nmosavari@gmail.com
3
Associate Professor, Bovine Tuberculosis Reference Laboratory, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
AUTHOR
KEYVAN
TADAYON
mmb093@gmail.com
4
Associate Professor, Bovine Tuberculosis Reference Laboratory, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
LEAD_AUTHOR
Abdolmohammadi Khiav, L., Haghkhah, M., Tadayon, K., & Mosavari, N. (2018). Isolation of Mycobacterium avium subsp. paratuberculosis and confirmation of cases by Nested-PCR. Veterinary Researches & Biological Products, 32(1), 41-47.
1
Baharsefat, M., Amjadi, A. R., Ahourai, P., Yamini, B., Entessar, F., & Hedayati, H. (1972). Paratuberculosis in goats and sheep in Iran. Epidemiological, clinical, pathological features and laboratory diagnosis. Archives of Razi Institute, 24(1), 49-61.
2
Bannantine, J. P., Wu, C. W., Hsu, C., Zhou, S., Schwartz, D. C., Bayles, D. O., et al. (2012). Genome sequencing of ovine isolates of Mycobacterium avium subspecies paratuberculosis offers insights into host association. BioMed Central Genomics, 13, 89.
3
Bohrer, V. L. (1972). On the relation of harvest methods to early agriculture in the Near East. Economic Botany, 26(2), 145-155.
4
Castellanos, E., Aranaz, A., & De Buck, J. (2010). Rapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types by use of real-time PCR and high-resolution melt analysis of the MAP1506 locus. Journal of Clinical Microbiology, 48(4), 1474-7.
5
Castro, C., González, L., Rozo, J. C., Puerto, G., & Ribón, W. (2009). Biosafety evaluation of the DNA extraction protocol for Mycobacterium tuberculosis complex species, as implemented at the Instituto Nacional de Salud, Colombia. Biomedica, 29(4), 561-566.
6
Collins, D. M., De Zoete, M., & Cavaignac, S. M. (2002). Mycobacterium avium subsp. paratuberculosis strains from cattle and sheep can be distinguished by a PCR test based on a novel DNA sequence difference. Journal of Clinical Microbiology, 40(12), 4760-4762.
7
Collins, M. T., Kenefick, K. B., Sockett, D. C., Lambrecht, R. S., McDonald, J., & Jorgensen, J. B. (1990). Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens. Journal of Clinical Microbiology, 28(11), 2514-2519.
8
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9
Dimareli-Malli, Z., Mazaraki, K., Stevenson, K., Tsakos, P., Zdragas, A., Giantzi, V. et al. (2013). Culture phenotypes and molecular characterization of Mycobacterium avium subsp. paratuberculosis isolates from small ruminants. Research in Veterinary Science, 95(1), 49-53.
10
Djonne, B., Pavlik, I., Svastova, P., Bartos, M., & Holstad, G. (2005). IS900 restriction fragment length polymorphism (RFLP) analysis of Mycobacterium avium subsp. paratuberculosis isolates from goats and cattle in Norway. Acta Veterinaria Scandinavica, 46(1-2), 13-8.
11
Dohmann, K., Strommenger, B., Stevenson, K., De Juan, L., Stratmann, J., Kapur, V. et al. (2003). Characterization of genetic differences between Mycobacterium avium subsp. paratuberculosis type I and type II isolates. Journal of Clinical Microbiology, 41(11), 5215-5223.
12
Galiero, A., Fratini, F., Mataragka, A., Turchi, B., Nuvoloni, R., Ikonomopoulos, J., & Cerri, D. (2016). Detection of Mycobacterium avium subsp. paratuberculosis in cheeses from small ruminants in Tuscany. International Journal of Food Microbiology, 217, 195-9.
13
Mobius, P., Hotzel, H., Rassbach, A., & Kohler, H. (2008). Comparison of 13 single-round and nested PCR assays targeting IS900, ISMav2, F57 and locus 255 for detection of Mycobacterium avium subsp. paratuberculosis. Veterinary Microbiology, 126(4), 324-33.
14
Mobius, P., Luyven, G., Hotzel, H., & Kohler, H. (2008). High genetic diversity among Mycobacterium avium subsp. paratuberculosis strains from German cattle herds shown by combination of IS900 restriction fragment length polymorphism analysis and mycobacterial interspersed repetitive unit-variable-number tandem-repeat typing. Journal of Clinical Microbiology, 46(3), 972-81.
15
Mohrekesh Haghighat, M., Shahmoradi, A. H., Tadayon, K., Keshavarz, R., Ghaderi, R., Sekhavati, M., & Mosavari, N. (2017). Molecular identification of Mycobacterium avium subspecies paratuberculosis 316 F and III & V strains by a multi-approach strategy. Veterinary Researches and Biological Products, 30(2), 89-100.
16
Momotani, E., Ozaki, H., Hori, M., Yamamoto, S., Kuribayashi, T., Eda, S., & Ikegami, M. (2012). Mycobacterium avium subsp. paratuberculosis lipophilic antigen causes Crohn's disease-type necrotizing colitis in mice. SpringerPlus, 1(47), 1-10.
17
O'Mahony, J. & Hill, C. (2002). A real time PCR assay for the detection and quantitation of Mycobacterium avium subsp. paratuberculosis using SYBR Green and the Light Cycler. Journal of Microbiological Methods, 51(3), 283-293.
18
Schonenbrucher, H., Abdulmawjood, A., Failing, K., & Bulte, M. (2008). New triplex real-time PCR assay for detection of Mycobacterium avium subsp. paratuberculosis in bovine feces. Applied and Environmental Microbiology, 74(9), 2751-2758.
19
Singh, A. V., Chauhan, D. S., Singh, A., Singh, P. K., Sohal, J. S., & Singh, S. V. (2015). Application of IS1311 locus 2 PCR-REA assay for the specific detection of 'Bison type' Mycobacterium avium subspecies paratuberculosis isolates of Indian origin. Indian Journal of Medical Research, 141(1), 55-61.
20
Sohal, J. S., Singh, S. V., Singh, A. V., & Singh, P. K. (2010). Strain diversity within Mycobacterium avium subspecies paratuberculosis--a review. Indian Journal of Experimental Biology, 48(1), 7-16.
21
Sting, R., Hrubenja, M., Mandl, J., Seemann, G., Salditt, A., & Waibel, S. (2014). Detection of Mycobacterium avium subsp. paratuberculosis in faeces using different procedures of pre-treatment for real-time PCR in comparison to culture. Veterinary Journal, 199(1), 138-142.
22
VLA. (2008). Johne's disease continues to be the most common cause of bovine enteric disease, Veterinary Record, 163(6), 171-174.
23
Walton, C. L. (1917). Some geographical aspects of the sheep industry. Scottish Geographical Magazine, 33(7), 303-315.
24
Whipple, D., Kapke, P., & Vary, C. (1990). Identification of restriction fragment length polymorphisms in DNA from Mycobacterium paratuberculosis. Journal of Clinical Microbiology, 28(11), 2561-2564.
25
Windsor, P. A. (2015). Paratuberculosis in sheep and goats. Veterinary Microbiology, 181(1-2), 161-169.
26
Wolf, R., Barkema, H. W., De Buck, J., & Orsel, K. (2015a). Factors affecting management changes on farms participating in a Johne's disease control program. Journal of Dairy Sciences, 98(11), 7784-96.
27
Wolf, R., Barkema, H. W., De Buck, J., & Orsel, K. (2015b). Sampling location, herd size, and season influence Mycobacterium avium ssp. paratuberculosis environmental culture results. Journal of Dairy Sciences, 98(1), 275-87.
28
Zikovitz, A. E., Stalis, I. H., Bicknese, E. J., Rideout, B. A., & Pye, G. W. (2018). Resolution of a Localized Granuloma Caused by Mycobacterium avium-intracellulare Complex on the Cere of a Bruce's Green Pigeon (Treron waalia). Journal of Avian Medicine and Surgery, 32(4), 322-327.
29
ORIGINAL_ARTICLE
Study of phylogenetic background and antibiotic resistance of Escherichia coli isolates obtained from healthy household dogs and their owners in Kerman city
The dogs' relationship with their owners has made them one of the most popular pets. They can be a reservoir of many microbial pathogens, so they are important for public health. This study was performed to determine the phylogenetic backgrounds and antimicrobial resistance of E. coli isolates from healthy household dogs and their owners in Kerman province, Iran. Samples were taken regardless of antibiotic usage in the dogs. Based on the history of the animals, 90% of them had not used antibiotics during the few months before the study. 168 E. coli strains belonging to feces of healthy household dogs (n=49), their owners (n=49) and the people without a pet (n=70) were studied; phylogenetic sequences including chuA, yjaA and TspE4.C2 were screened by conventional polymerase chain reaction (PCR). The isolates were investigated phenotypically for antimicrobial resistance against 11 commonly used antibiotics in dogs which were erythromycin, streptomycin, enrofloxacin, oxytetracycline, cefotaxime, ampicillin, trimethoprim-sulfamethoxazole, amoxicillin-clavulanic acid, ceftazidime, ceftriaxone and kanamycin. E. coli isolates were classified into A, D, B1 and B2 phylogroups with the prevalence of 55.9%, 30.3%, 7.1% and 5.3%, respectively. Considerably, antimicrobial resistance to erythromycin and oxytetracycline were predominant while the lowest frequency of antibiotic resistance was detected against ceftriaxone, ceftazidime, amoxicillin-clavulanic acid and kanamycin. This study was performed on apparently healthy dogs so it could determine their carrier role for antibiotic-resistant E. coli strains. This research revealed that healthy household dogs should be considered as the significant reservoir of resistant E. coli isolates especially to erythromycin, oxytetracycline, streptomycin, enrofloxacin, cefotaxime and ampicillin which these resistant strains were mostly belonging to A and D phylogenetic groups.
https://www.ivj.ir/article_128899_e546df3fb5338abf742bfd05e0e36a75.pdf
2021-02-19
55
63
10.22055/ivj.2019.195428.2169
Escherichia coli
Dog
Owners
Antimicrobial Resistance
Phylogenetic
Asma
Askari
askari_asma@yahoo.com
1
Researcher, Research Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences and PhD Graduated in Bacteriology, Department of Pathobiology, Faulty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
AUTHOR
Reza
Ghanbarpour
ghanbar@uk.ac.ir
2
Professor, Molecular Microbiology Research Group, Shahid Bahonar University of Kerman, Kerman, Iran
LEAD_AUTHOR
Mohammad Reza
Aflatoonian
mraflatoonian@yahoo.com
3
Assistant Professor, Research Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran
AUTHOR
Baharak
Akhtardanesh
akhtardanesh@uk.ac.ir
4
Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
AUTHOR
Hamid
Sharifi
sharifihami@gmail.com
5
Professor, HIV/STI Surveillance Research Center, and WHO Collaborating Center for HIV Surveillance, Institute for Futures Studies in Health and Professor, Department of Biostatistics and Epidemiology, Faculty of Public Health, Kerman
AUTHOR
Maziar
Jajarmi
maziar.jajarmi@uk.ac.ir
6
Assistant Professor, Department of Pathobiology, Faulty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
AUTHOR
Akhtardanesh, B., Ghanbarpour, R., Ganjalikhani, S., & Gazanfari, P. (2016). Determination of antibiotic resistance genes in relation to phylogenetic background in Escherichia coli isolates from fecal samples of healthy pet cats in Kerman city. Veterinary Research Forum, 7(4), 301-308.
1
Alizade, H. (2018). Escherichia coli in Iran: An overview of antibiotic resistance: A review article. Iranian Journal of Public Health, 47(1), 1-12.
2
Alizade, H., Jajarmi, M., Aflatoonian, M. R., Kalantar-Neyestanaki, D., Shoja, S., & Ghanbarpour, R. (2018). Comparative prevalence of blactx-m-15 gene with virulence genes and serotypes in Klebsiella pneumoniae. Jundishapur Journal of Microbiology, 11(4), e61285.
3
Amiri, M., Jajarmi, M., & Ghanbarpour, R. (2017). Prevalence of resistance to quinolone and fluoroquinolone antibiotics and screening of qnr genes among Escherichia coli isolates from urinary tract infection. International Journal of Enteric Pathogen, 5 (4), 100-105.
4
Carvalho, A. C., Barbosa, A. V., Arais, L. R., Ribeiro, P. F., Carneiro, V. C., & Cerqueira, A. M. F. (2016). Resistance patterns, ESBL genes, and genetic relatedness of Escherichia coli from dogs and owners. Brazilian Journal of Microbiology, 47 (1), 150-158.
5
Clermont, O., Bonacorsi, S., & Bingen, E. (2000). Rapid and simple determination of theEscherichia coli phylogenetic group. Applied and environmental microbiology, 66 (10), 4555-4558.
6
Clermont, O., Christenson, J. K., Denamur, E., & Gordon, D. M. (2013). The C lermont E scherichia coli phylo‐typing method revisited: improvement of specificity and detection of new phylo‐groups. Environmental microbiology reports, 5 (1), 58-65.
7
CLSI. (2018). Performance Standards for Antimicrobial Susceptibility Testing (28th ed). Clinical and Laboratory Standards Institute supplement M100S. Wayne, PA, US. Pp:52-59.
8
Damborg, P., Nielsen, S. S., & Guardabassi, L. (2009). Escherichia coli shedding patterns in humans and dogs: insights into within-household transmission of phylotypes associated with urinary tract infections. Epidemiology & Infection, 137 (10), 1457-1464.
9
Duriez, P., Clermont, O., Bonacorsi, S., Bingen, E., Chaventre, A., Elion, J. et. al. (2001). Commensal Escherichia coli isolates are phylogenetically distributed among geographically distinct human populations. Microbiology, 147(6), 1671-1676.
10
Escobar-Páramo, P., Grenet, K., Le Menac'h, A., Rode, L., Salgado, E., Amorin, C. et al. (2004). Large-scale population structure of human commensal Escherichia coli isolates. Applied and Environmental Microbiology 70 (9), 5698-5700.
11
Gasparrini, A. J., Crofts, T. S., Gibson, M. K., Tarr, P. I., Warner, B. B., & Dantas, G. (2016). Antibiotic perturbation of the preterm infant gut microbiome and resistome. Gut Microbes, 7(5), 443-449.
12
Ghanbarpour, R., Akhtardanesh, B., Afsahi, E., & Sookhtanloo, S. (2010). Molecular characterization of Escherichia coli pathotypes from diarrheic and healthy dogs. Online Journal of Veterinary Research, 14(2), 316-324.
13
Girardeau, J. P., Dalmasso, A., Bertin, Y., Ducrot, C., Bord, S., Livrelli, V., Vernozy-Rozand, C., & Martin, C. (2005). Association of virulence genotype with phylogenetic background in comparison to different seropathotypes of Shiga toxin-producing Escherichia coli isolates. Journal of Clinical Microbiology, 43(12), 6098-6107.
14
Harada, K., Morimoto, E., Kataoka, Y., & Takahashi, T. (2011). Clonal spread of antimicrobial-resistant Escherichia coli isolates among pups in two kennels. Acta Veterinaria Scandinavica, 53(1), 1-7.
15
Harada, K., Niina, A., Nakai, Y., Kataoka, Y., & Takahashi, T. (2012). Prevalence of antimicrobial resistance in relation to virulence genes and phylogenetic origins among urogenital Escherichia coli isolates from dogs and cats in Japan. American Journal of Veterinary Research, 73(3), 409-417.
16
Harada, K., Okada, E., Shimizu, T., Kataoka, Y., Sawada, T., & Takahashi, T. (2012). Antimicrobial resistance, virulence profiles, and phylogenetic groups of fecal Escherichia coli isolates: A comparative analysis between dogs and their owners in Japan. Comparative Immunology, Microbiology and Infectious Diseases, 35(2), 139-144.
17
Hutton, R. (2018). Antibiotic resistance in small animal veterinary practice: veterinary nurses as antibiotic guardians. The Veterinary Nurse, 9 (1), 4-10.
18
Jang, J., Hur, H.G., Sadowsky, M.J., Byappanahalli, M.N., Yan, T., & Ishii, S. (2017). Environmental Escherichia coli: ecology and public health implications—a review. Journal of Aplied Microbiology, 123(3), 570-581.
19
Johnson, J. R., Kuskowski, M. A., Owens, K., Clabots, C., & Singer, R. S. (2009). Virulence genotypes and phylogenetic background of fluoroquinolone-resistant and susceptible Escherichia coli urine isolates from dogs with urinary tract infection. Veterinary Microbiology, 136 (1-2), 108-114.
20
Jorgensen, J. H., & Turnidge, J. D. (2015). Susceptibility test methods: dilution and disk diffusion methods. Manual of clinical microbiology (11th Edition). American Society for Microbiology (ASM). Washington, US. Pp:1253-1273.
21
Markey, B., Leonard, F., Archambault, M., Cullinane, A. & Maguire, D. (2013). Clinical veterinary microbiology (2nd Edition). Elsevier Health Sciences. Pp: 239-274.
22
Skurnik, D., Le Menac'h, A., Zurakowski, D., Mazel, D., Courvalin, P., Denamur, E., et al. (2005). Integron-associated antibiotic resistance and phylogenetic grouping of Escherichia coli isolates from healthy subjects free of recent antibiotic exposure. Antimicrobial Agents and Chemotherapy, 49 (7), 3062-3065.
23
Sperandio, V. & Hovde, C. J. (2015). Enterohemorrhagic Escherichia coli and other Shiga toxin-producing E. coli (1st ed). American Society for Microbiology (ASM). Washington, US. Pp:552-559.
24
Stenske, K. A., Bemis, D. A., Gillespie, B. E., D'Souza, D. H., Oliver, S. P., Draughon, F. A., et al. (2009). Comparison of clonal relatedness and antimicrobial susceptibility of fecal Escherichia coli from healthy dogs and their owners. American Journal of Veterinary Research, 70(9), 1108-1116.
25
Stokes, H. W., & Gillings, M. R. (2011). Gene flow, mobile genetic elements and the recruitment of antibiotic resistance genes into Gram-negative pathogens. FEMS Microbiology Reviews, 35(5), 790-819.
26
Tenaillon, O.; Skurnik, D.; Picard, B. & Denamur, E. (2010). The population genetics of commensal Escherichia coli. Nature reviews microbiology, 8(3), 207-217.
27
Unno, T., Han, D., Jang, J., Widmer, K., Ko, G., Sadowsky, M. J., & Hur, H. G. (2009). Genotypic and phenotypic trends in antibiotic resistant pathogenic Escherichia coli isolated from humans and farm animals in South Korea. Microbes and Environments, 26(3), 198-204.
28
Wayne, A., McCarthy, R., & Lindenmayer, J. (2011). Therapeutic antibiotic use patterns in dogs: observations from a veterinary teaching hospital. Journal of Small Animal Practice, 52(6), 310-318.
29
Xie, W.Y., Shen, Q., & Zhao, F. J. (2018). Antibiotics and antibiotic resistance from animal manures to soil: a review. European Journal of Soil Science, 69(1), 181-195.
30
Zogg, A. L., Zurfluh, K., Schmitt, S., Nüesch-Inderbinen, M., & Stephan, R. (2018). Antimicrobial resistance, multilocus sequence types and virulence profiles of ESBL producing and non-ESBL producing uropathogenic Escherichia coli isolated from cats and dogs in Switzerland. Veterinary Microbiology, 216, 79-84.
31
ORIGINAL_ARTICLE
The effect of substitution to DL-methionine with L-methionine and dietary protein levels on Expression Myogenic Genes in Japanese quail
This research was conducted to investigate the effect of different levels of protein (20 and 24%) and the replacement of methionine DL with L methionine on the expression of myogenic genes (atrogin-1 and MYF-5) using the RT-qPCR technique in Japanese quail. This experiment was done in the form of a 2×2 factorial with 4 treatments and 4 repetitions and 15 quail in each replicate. The first treatment included DL-methionine and 20% protein (control group). The second treatment consisted of L methionine and 24% proteins, and the third treatment included L methionine and protein 20% and the fourth treatment included DL-methionine and protein 24%. After 35 days of feeding and keeping the quails, with 8 hours interval of hunger, 2 quails were slaughtered in each replicate, and a piece of their chest has been removed immediately and was transferred to the laboratory with Liquid nitrogen, and froze in -80°c. After extraction of the whole RNA, its quality was measured and was used to generate and synthesis the cDNA. Eventually, the expression of myogenic genes was measured by the real-time PCR method. In this method, 𝜷-actin gene, as the source gene, was used to normalize the data. The results showed that by decreasing the protein level from 24% to 20%, atrogin-1 gene expression increased and the MYF-5 gene expression decreased. Also, the replacement of methionine DL with L-methionine did not have a significant effect on the expression of myogenic genes. The results indicated that DL-methionine could be replaced with L methionine, and a 24% protein level is more suitable than 20% in the Japanese quail diet.
https://www.ivj.ir/article_128900_2d3768eab8bacb97a795ed6a334c81ab.pdf
2021-02-19
64
73
10.22055/ivj.2019.152644.2078
Japanese quail
Atrogin-1
Gene expression
Myogenic genes
kei aram
koohgivi
kia.koohgivi@yahoo.com
1
MSc Graduated of Animal genetic and Breeding, Faculty of Animal Science and Food Technology, Agricultural science and Natural Resources University of Khuzestan, Mollasani, Iran
AUTHOR
Hedayatollah
Roshanfekr
roshanfekr_hd@yahoo.com
2
Professor, Department of Animal Science, Faculty of Animal science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Mollasani, Iran
AUTHOR
mahmood
nazari
fat_sa_2005@yahoo.com
3
Assistant Professor, Department of Animal Science, Faculty of Animal science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Mollasani, Iran
LEAD_AUTHOR
Ahmad
Tatar
ahmadtatar@gmail.com
4
Assistant Professor, Department of Animal Science, Faculty of Animal science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Mollasani, Iran
AUTHOR
Aghaei, A., Khosravinia, H., Mamoei, M., Azarfar, A., Shahriari, A., and Ghorbanpor, M. (2018). Effects supplementation of zinc and Vit E on antioxidant enzyme, sexual hormone and some biochemical parameters in breeder flock of Japanese Quails. Iranian Veterinary Journal, 4 (2), 14-24. (In Persian)
1
Dersjant‐Li, Y. and Peisker, M. (2011). A Review on Recent Findings on Amino Acids Requirements in Poultry Studies. Iranian Journal of Applied Animal Science, 1(2), 73-79.
2
Dilger, R.N., Kobler, C., Weckbecker, C., Hoehler, D. and Baker, D.H. (2007). 2-keto-4-(Methylthio) butyric acid (Keto analog of methionine) is a safe and efficacious precursor of L-Methionine in chicks. Journal of Nutrition, 137, 1868-1873.
3
Evonik Industries, AG Health and & Nutrition (2015). Nutritional value of L-Methionine is comparable to DL-Methionine in growing broilers from 14 to 27 days of age. Facts & Figures, 15113.
4
Hyankova, L., Dedkova, L., Knizetva, H. and Klecner, D. (1997). Responses in growth, food intake and food conversion efficiency to different dietary protein concentrations in meat-type lines of Japanese quail. British Poultry Science, 38, 564-570.
5
Huyghebaert, G. (1993). Comparison of DL-methionine and methionine hydroxy analog-free acid in broilers by using multi exponential regression models. British Poultry Science, 34, 351–359.
6
Kaur, S., Mandal, A., Singh, K. and Kadam, M. (2008). The response of Japanese quails (heavy body weight line) to dietary energy levels and graded essential amino acid levels on growth performance and immunocompetence. Livestock Science, 117, 255-262.
7
Lee, J., Giordano, S. and Zhang, J. (2012). Autophagy, mitochondria and oxidative stress: cross-talk and redox signaling. Journal of Biochemistry, 441, 523–540.
8
Nasiri Moghadam, H., Hesabi Nameghi, A. and Madayeni, M.M. (2007). Effect of supplementation of methionine and lysine amino acids on yield and carcass characteristics of broiler chicks. Journal Iranian Agriculture, 20, 183-192. (In Persian)
9
National Research Council. (1994). Nutrition of Poultry. National Academy Press. Washington D. C., U.S.A.
10
Parvin, R., Mandal, A.B., Singh, S.M. and Thakur, R. (2010). Effect of dietary level of methionine on growth performance and immune response in Japanese quails (Coturnix coturnix japonica). Journal Science Food Agriculture, 90, 471-481.
11
Pfaffl, M.W., Horgan, G.W. and Dempfle, L. (2002). Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Research, 30, pp.1-10.
12
Ribeiro, A., Dahlke, F. and Kessler, A.M. (2005). Methionine sources do not affect performance and carcass yield of broilers fed vegetable diets and submitted to cyclic heat stress. Brazilian Journal of Poultry Science, 7(3), 159-164.
13
Sabourin, L.A. and Rudnicki, M.A. (2000). The molecular regulation of myogenesis. Clinical Genetics, 57(1), 16-25.
14
SAS Institute, (1999). SAS/STAT Users Guide. SAS Inc, NC.
15
Shen, Y.B., Ferket, P., Park, I., Malheiros, R.D. and Kim, S.W. (2015). Effects of feed grade l-methionine on intestinal redox status, intestinal development, and growth performance of young chickens compared with conventional dl-methionine. Journal of Animal Science, 93, 2977-2986.
16
Shen, Y.B., Weaver, A.C. and Kim, S.W. (2014). Effect of feed grade L-Methionine on growth performance and gut health in nursery pigs compared to conventional DL-Methionine. Journal of Animal Science, 92, 5530-5539.
17
Vesco, A.P., Gasparino, E., Oliveira Neto, A.R., Guimarães, S.E. and Marcato, S.M. (2015). Effects of Methionine Supplementation on the Expression of Protein Deposition-Related Genes in Acute Heat Stress-Exposed Broilers. PLoS One, 10(2), e0115821.
18
Wen, C., Chen, X., Chen, G.Y., Wu, P., Chen, Y.P., Zhou, Y.M. and Wong, T. (2014). Methionine improves breast muscle growth and alters myogenic gene expression in broilers. Journal of Animal Science 92(3), 1068-1073.
19
ORIGINAL_ARTICLE
Easy, low cost, and precise Identification and interpretation of Newcastle virus and differentiation of its velogenic from lentogenic strains by using the nested RT-ARMS PCR
Newcastle disease is a viral disease of poultry that is caused by type 1 paramyxovirus, from the Avulavirus genus and its fast and precise diagnosis is of high importance. Virulence of this virus depends on Fusion protein (F), one of six proteins of this virus, which can be used for detection of the virus virulence. So, this study aims to design new primers according to bioinformatics science progression and suggest a cheaper and easier method for the identification of Newcastle virus (NDV) as well as distinguish lentogenic from velogenic strains with higher sensitivity and specificity. First, all available strains of Newcastle viruses were collected from NCBI data bank using Blast tool and after multiple alignments, universal and specific primers were designed. In the next step, identification of NDV was set up using universal primers by PCR on cDNA of the control positive sample. Then differentiation of lentogenic from velogenic strains set up by ARMS (Amplification Refractory mutation system)-PCR (Polymerase chain reaction) using specific primers. Because the method was performed on cDNA obtained from reverse transcription reaction (RT), and because the PCR product of the first PCR reaction was used as a template for nested second PCR reaction it is called “nested RT-ARMS PCR”. Afterward, some samples from broiler farms were tested by this method and then compared by Real-Time PCR as a golden standard test. The results showed that sensitivity and specificity of identification of the virus and its strains were fully compatible in both methods. To sum up, this method which consumes a little bit more time but lower expenses, equipment and complexity in comparison with Real-Time PCR, can be suggested as a suitable substitution for the detection of NDV and distinguishing its velogenic from lentogenic strains.
https://www.ivj.ir/article_128901_e9a0cf9cea4198e466ee71b5354a470f.pdf
2021-02-19
74
83
10.22055/ivj.2019.185474.2135
Newcastle
F protein
Velogenic
Lentogenic
Nested RT-ARMS PCR
Maryam
Mohammadi-Dastenaei
mohammadi_vip@yahoo.com
1
DVM Graduated, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
AUTHOR
Abdolkarim
Zamani Moghaddam
azamani2@yahoo.com
2
Professor, Department of Clinical Science, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
AUTHOR
Mostafa
Shakhsi-Niaei
shakhsi-niaei.m@sku.ac.ir
3
Assistant Professor, Department of Genetics, Faculty of Basic Sciences, Shahrekord University, and Research Institute of Biotechnology, Shahrekord University, Shahrekord, Iran
LEAD_AUTHOR
Alexander, D.J. (2003). Newcastle disease. In: Disease of poultry (1st ed). Iowashate university press. Iowa, Pp: 64-87.
1
Alexander, D.J., & Senne D.A. (2008). Newcastle disease,other Avain paramyxoviruses, and pneumovirus infection (12th ed., pp:75-115). Blackwell, Ames, Iowa.
2
Baratchi, S., Ghorashi, S.A., Hosseini, M., & Pourbakhsh S.A. (2003). Differentiation of virulent and non-virulent Newcastle disease virus isolates using RT-PCR. Iranian Journal of Biotechnology, 4(1), 61-63.
3
Fuller, C.M., Collins, M.S., Easton, A.J., & Alexander, D.J. (2007). Partial characterization of five cloned viruses differing in pathogenicity, obtained from a single isolate of pigeon paramyxovirus type1 (PPMV-1) following passage in fowls eggs. Archives of Virology, 152(8), 1575-82.
4
Pham, H.M., Konnai, S., Usui, T., Chang, K.S., Murata, S. Mase, M., Ohashi, K., & Onuma, M. (2005). Rapid detection and differentiation of Newcastle disease virus by Real-Time PCR with melting curve analysis. Archives of Virology, 150(12), 2429-2438.
5
Kant, A., Koch, G., Van Roozelaar, D.J., Balk F., & Ter Huurne, A. (1997). Differentiation of virulent and non‐virulent strains of Newcastle disease virus within 24 hours by polymerase chain reaction, Avian Pathology, 26(4), 837-849.
6
Kianizadeh, M., Aini, I., Omar, A.R., Yusoff, K., & Sahrabadi, M. (2002). Sequence and phylogenetic analysis of the fusion protein cleavage site of Newcastle disease virus field isolates from Iran, Acta virologica, 46(4), 247-51.
7
Kovics, S., & Horvath, J. (2000). Newcastle disease virus (NDV): brief history of its oncolytic strains, Journal of Clinical Virology, 16(1), 1-15.
8
Mase, M., Imai, K., Sanada, Y., Sanada, N., Yuasa, N., Imada, T., Tsukamoto, K., & Yamaguchi, S. (2002). Phylogenetic analysis of Newcastle Disease Virus Genotypes Isolated in Japan, Journal of Clinical Virology, 40(10), 3826–3830.
9
Wise, M.G., Suarez, D.L., Seal, B.S., Pedersen, J.C., Senne, D.A., King, D.J., Kapczynski, D.R., & Spackman, E. (2003). Development of a Real-Time Reverse Transcription PCR for Detection of Newcastle disease virus RNA in clinical Samples, Journal of Clinical Microbiology, 42(1), 329-38.
10
Naghavi, M. R., Gharehyazi, B., & Hosseini-Salcadeh, G.H. (2013). Molecular Markers (4th ed). Tehran University press. Iran, Pp:160.
11
Newton, C.R., Graham, A., Heptinstall, L.E., Powell, S.J., Summers, C., Kalsheker, N., Smith, J.C., & Markham, A.F. (1989). Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS). Nucleic Acids Res, 17(7), 2503–2516.
12
Office International des Epizooties. (2001). Newcastle disease. Manual of standards for diagnostic tests and vaccines (4th ed), Paris: OLG.
13
Snoeck, C.J., Owoade, A.A., Hymann, E.C., Alkali, B.R., Okwen, M.P., Adeyanju, A.T., Komoyo, G.F., Nakouné, E., Le Faou, A., & Muller, C.P. (2013). High genetic diversity of Newcastle disease virus in poultry in west and central Africa: Cocirculation of genotype XIV and newly definded genotypes XVII and XVIII. Journal of Clinical Microbiology, 51(7), 2250-60.
14
Swayne, D.E., Glisson, J.R., & Jackwood, M.W. (1998). A laboratory manual for the isolation and identification of avian pathogens (4th ed). Pennsylvania, Pennsylvania University Press, US. Pp:1563-1630.
15
Vahidi, V., Zolfaghari, M., Ahmadi, A., shojapour, M., Moaddab, S.R., & Arjomandzadegan, M. (2014). Detecion of opioxacin resistance by rapid Molecular Method of Allele Specific PCR in Mycobacterium tuberculosis. Biological Journal of Microorganisms, 3(9), 21-34.
16
Veronique, J., & Jestin, A. (1990). Detection of Newcastle disease virus RNA in infected allantoic fluids by in vitro enzymatic amplification (PCR), Archives Virology, 118,151-161.
17
ORIGINAL_ARTICLE
A survey on the sedation effects of medetomidine, dexmedetomidine and their combination with acepromazine on cardiovascular function in dog
Medetomidine and dexmedetomidine are the two new alpha-2 agonists available for use in veterinary anesthesia. These drugs are employed for sedation and premedication in small animals. The present study aimed to investigate the effects of medetomidine and dexmedetomidine alone and in combination with acepromazine on sedation, cardiovascular function and electrocardiography in dogs. Sixty dogs were randomly divided into four equal groups. The dogs received one of the treatments of medetomidine (10 μg/kg), dexmedetomidine (5 μg/kg), medetomidine (10 μg/kg) with acepromazine (0.05 mg/kg) and dexmedetomidine (5 μg/kg) with acepromazine (0.05 mg/kg) intramuscularly. Sedation levels, heart rate, non-invasive arterial blood pressure, respiratory rate, body temperature and electrocardiogram were carefully recorded in dogs up to 20 minutes after administration. Sedation scores were significantly higher in groups of medetomidine with acepromazine and dexmedetomidine with acepromazine, at 5 and 20 minutes in comparison to groups of medetomidine and dexmedetomidine alone. Comparison of sedation scores in each group showed a significant increase over time. The comparison of heart rate, within the groups, showed a significant decrease when compared with the baseline value. The respiratory rate showed a decreasing trend in all groups over time. The amplitude of the P wave decreased and the P-R and Q-T intervals increased during the evaluation period in all groups. Sinus arrhythmia, AV-block grade 1, and sinus arrest were seen after the administration of sedative drugs in all groups. In conclusion, it is inferred that the addition of acepromazine to medetomidine and dexmedetomidine increases the sedation level. Heart rate decreased in medetomidine and dexmedetomidine groups and the addition of acepromazine exacerbated this decrease. The combination of acepromazine with medetomidine and dexmedetomidine did not also reduce the occurrence of arrhythmias in the dog.
https://www.ivj.ir/article_128904_a19de3d68768c3bcf4a94c99c5eef4c4.pdf
2021-02-19
84
96
10.22055/ivj.2019.189127.2146
Acepromazine
Medetomidine
dexmedetomidine
Cardiovascular function
Dog
Bahman
Mosallanejad
bmosallanejad@scu.ac.ir
1
Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
LEAD_AUTHOR
Hadi
Imani
h.imani@scu.ac.ir
2
Assistant professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
Ali
Baniadam
baniadam@scu.ac.ir
3
Associate professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
Farhad
Saripour
farhad.saripour@gmail.com
4
Graduated, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
Ahmad, R. A., Kinjavdekar, P., Aithal, H. P., Pawde, A. M., & Kumar, D. (2013). Potential use of dexmedetomidine for different levels of sedation, analgesia and anaesthesia in dogs. Veterinarni Medicina, 58(2), 87-95.
1
Alvaides, R. K., Neto, F. T., Aguiar, A. J. D. A., Campagnol, D., & Steagall, P. V. M. (2008). Sedative and cardiorespiratory effects of acepromazine or atropine given before dexmedetomidine in dogs. Veterinary Record, 162(26), 852-856.
2
Avdosko, G., Birgele, E., & Mencendorfa, L. (2010). Changes in electrocardiogram of Cocker Spaniel and German Shepherd dogs during anesthesia. Veterinarija Ir Zootechnika, 49(71), 8-16.
3
Borges, L. B. P., Nishimura, L. T., Carvalho, L. L., Cerejo, S. A., Auckburally, A., & Mattos-Junior, E. (2016). Behavioral and cardiopulmonary effects of dexmedetomidine alone and in combination with butorphanol, methadone, morphine or tramadol in conscious sheep. Veterinary Anaesthesia and Analgesia, 43(5), 549–560.
4
Cardoso, C. G., Marques, D. R., Da Silva, T. H. M., & De Mattos-Junior, E. (2014). Cardiorespiratory, sedative and antinociceptive effects of dexmedetomidine alone or in combination with methadone, morphine or tramadol in dogs. Veterinary Anaesthesia and Analgesia, 41(6), 636–643.
5
Cardoso, H. M., Padilha, V. S., Tocheto, R., Regalin, D., Gehrcke, M. I., & Oleskovicz, N. (2016). Electrocardiographic, echocardiographic, and indirect blood pressure evaluation in dogs subjected to different sedation protocols. Ciencia Rural, 46(11), 2043-2048.
6
Congdon, J. M., Marquez, M., Niyom, S., & Boscan, P. (2011). Evaluation of the sedative and cardiovascular effects of intramuscular administration of dexmedetomidine with and without concurrent atropine administration in dogs. Journal of the American Veterinary Medical Association, 239(1), 81-89.
7
Doyle, D. J., & Garmon, E. H. (2019). American Society of Anesthesiologists classification (ASA class). In Stat Pearls [Internet], Stat Pearls Publishing. Available from: https://europepmc.org.
8
Gomez Villamandos, R., Palacios, C., Benitez, A., Granados, M. M., Domínguez, J. M., Lopez, I., Ruiz, I., Aguilera, E., & Santisteban, J. M. (2006). Dexmedetomidine or medetomidine premedication before propofol–desflurane anaesthesia in dogs. Journal of Veterinary Pharmacology and Therapeutics, 29(3), 157-163.
9
Granholm, M., McKusick, B. C., Westerholm, F. C., & Aspegren, J. C. (2007). Evaluation of the clinical efficacy and safety of intramuscular and intravenous doses of dexmedetomidine and medetomidine in dogs and their reversal with atipamezole. Veterinary Record, 160(26), 891-897.
10
Hanton, G., & Rabemampianina, Y. (2006). The electrocardiogram of the Beagle dog: reference values and effect of sex, genetic strain, body position and heart rate. Laboratory Animals, 40(2), 123-136.
11
Huupponen, E., Maksimow, A., Lapinlampi, P., Sarkela, M., Saastamoinen, A., Snapir, A., Scheinin, H., Scheinin, M., Merilainen, P., Himanen, S. L., & Jaaskelainen, S. (2008). Electroencephalogram spindle activity during dexmedetomidine sedation and physiological sleep. ACTA Anaesthesiology Scandinavica, 52(2), 289-294.
12
Ko, J. C. H., Fox, S. M., & Mandsager, R. E. (2000). Sedative and cardiorespiratory effects of medetomidine, medetomidine-butorphanol, and medetomidine-ketamine in dogs. Journal of the American Veterinary Medical Association, 216(10), 1578-1583.
13
Kuo, W. C., & Keegan, R. D. (2004). Comparative cardiovascular, analgesic and sedative effects of medetomidine, medetomidine-hydromorphone, and medetomidine-butorphanol in dogs. American Journal of Veterinary Research, 65(7), 931-937.
14
Kuusela, E., Raekallio, M., Vaisanen, M., Mykkanen, K., Ropponen, H., & Vainio, O. (2001). Comparison of medetomidine and dexmedetomidine as premedicants in dogs undergoing propofol-isoflurane anesthesia. American Journal of Veterinary Research, 62(7), 1073-1080.
15
Lemke, K. A. (2004). Perioperative use of selective alpha-2 agonists and antagonists in small animals. Canadian Veterinary Journal, 45(6), 475-480.
16
Leppanen, M. K., Mc Kusick, B. C., Granholm, M. M., Westerholm, F. C., Tulamo, R., & Short, C.E. (2006). Clinical efficacy and safety of dexmedetomidine and buprenorphine, butorphanol or diazepam for canine hip radiography. The Journal of Small Animal Practice, 47(11), 663-669.
17
Monteiro, E. R., Figueroa, C. D., Choma, J. C., Campagnol, D., & Bettini, C. M. (2008). Effects of methadone, alone or in combination with acepromazine or xylazine, on sedation and physiologic values in dogs. Veterinary Anaesthesia and Analgesia, 35(6), 519-527.
18
Monteiro, E. R., Junior, A. R., Assis, H. M. Q., Campagnol, D., & Quitzan, J. G. (2009). Comparative study on the sedative effects of morphine, methadone, butorphanol or tramadol, in combination with acepromazine, in dogs. Veterinary Anaesthesia and Analgesia, 36(1), 25-33.
19
Puighibet, Z., Costa Farre, C., Santos, L., Canfran, S., & Gomez de Segura, I. A. (2015). The sedative effects of intramuscular low‐dose medetomidine in combination with butorphanol or methadone in dogs. Veterinary Anaesthesia and Analgesia, 42(6), 590-596.
20
Raszplewicz, J., MacFarlane, P., & West, E. (2013). Comparison of sedation scores and propofol induction doses in dogs after intramuscular premedication with butorphanol and either dexmedetomidine or medetomidine. Veterinary Anaesthesia and Analgesia, 40(6), 584-589.
21
Sarchahi, A. A., Vesal, N., Nikahval, B., & Karampour, A. (2009). Comparison of the effects of different doses of acepromazine-xylazine on the electrocardiogram in dogs. Iranian Journal of Veterinary Research, 10(3), 208-215.
22
Simoes, C. R., Monteiro, E. R., Rangel, J. P. P., Nunes-Junior, J. S., & Campagnol, D. (2016). Effects of a prolonged infusion of fentanyl, with or without atropine, on the minimum alveolar concentration of isoflurane in dogs. Veterinary Anaesthesia and Analgesia, 43(2), 136-144.
23
Sinclair, M. D. (2003) A review of the physiological effects of alpha 2-agonists related to the clinical use of medetomidine in small animal practice. Canadian Veterinary Journal, 44(11), 885–897.
24
Thomsen, M. B., Volders, P. G. A., Beekman, J. D. M., Matz, J., & Vos, M. A. (2006). Beat-to-beat variability of repolarization determines proarrhythmic outcome in dogs susceptible to drug-induced torsades de pointes. Journal of the American College of Cardiology, 48(6), 1268-1276.
25
Tilley, L. P., & Goodwin, J. K. (2001). Manual of Canine and Feline Cardiology (3th Edition). W.B. Saunders Company, Philadelphia, USA. Pp: 43-58.
26
Tilley, L. P., & Smith, F. W. K. (2000). The 5- Minute Veterinary Consult: Canine and Feline (2nd Edition). Lippincott Williams and Wilkins, Baltimore, USA. Pp: 298-300.
27
Valverde, A., Cantwell, S., Hernández, J., & Brotherson, C. (2004). Effects of acepromazine on the incidence of vomiting associated with opioid administration in dogs. Veterinary Anesthesia and Analgesia, 31(1), 40-45.
28
Vesal, N., Sarchahi, A. A., Nikahval, B., & Karampour, A. (2011). Clinical evaluation of the sedative properties of acepromazine xylazine combinations with or without atropine and their effects on physiologic values in dogs. Journal of the Faculty of Veterinary Medicine University of Zagreb, 81(4), 485-498.
29
ORIGINAL_ARTICLE
The effects of selenium nanoparticles and sodium selenite on transcription rate of resistin gene in ewes’ placenta
Resistin is a hormone secreted from adipose tissue that plays an important role in regulating energy homeostasis and glucose metabolism. In humans, only a small amount of resistin is expressed in adipose tissue and is most commonly found in the bone marrow, spleen, lung tissue, and placenta. The purpose of this study was to investigate the effect of oral administration of selenium and selenite sodium nanoparticles on the transcription rate of the resistin gene in pregnant ewes. For this, 20 pregnant ewes were randomly selected and divided into four groups and were administered daily for 10 days leading up to birth. In treated group 1 sodium selenite (0.1 mg/kg body weight), while in treated groups 2 and 3 selenium nanoparticles (at doses of 0.05 and 0.1 mg/kg body weight, respectively) were administered. The fourth group was received distilled water and served as a control group. At the time of parturition, samples were taken from the placenta and the transcription rate of the resistin gene was determined by RT-PCR Real-Time based on a comparison assay of 2-ΔΔCt. The results showed that oral selenium administration to pregnant ewes caused a significant increase in the amount of resistin gene transcription rate which the role of sodium selenite was more pronounced than that of selenium nanoparticles (With p, equal to 0.002, 0.002 and 0.001 respectively).
https://www.ivj.ir/article_128908_b476cb8b7a8a9eb61416069ddab435fc.pdf
2021-02-19
97
107
10.22055/ivj.2019.125104.2029
placenta
Transcription rate
selenium nanoparticles
Resistin
Pedram
Moayeri
dr.moayeri@hotmail.com
1
PhD Graduated in large animal internal medicine, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran
LEAD_AUTHOR
Gholam Ali
Kojouri
drgholam_alikojouri@yahoo.com
2
Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran
AUTHOR
afshin
jafari dehkordi
jafari-a@vet.sku.ac.ir
3
Associated Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran
AUTHOR
ali mohammad
ahadi
ahadi52@gmail.com
4
Assistant Professor, Department of Genetics, Faculty of Science, University of Shahrekord, Shahrekord, Iran
AUTHOR
Mahsa
Abolfazlzadeh
5
Graduated in Nursing, Astara branch of Islamic Azad University
AUTHOR
Ahima, R.S. and Flier, J.S. (2000). Adipose tissue as an endocrine organ. Trends in Endocrinology and Metabolism, 11(8): 327-332.
1
Bauersachs, S.; Kirchgessner, M. and Paulicks, B. (1993) Effects of different levels of dietary selenium and vitamin E on the humoral immunity of rats. Journal of trace elements and electrolytes in health and disease, 7(3):147-152.
2
Behne, D.; Höfer, T.; von Berswordt-Wallrabe, R. and Elger, W. (1982). Selenium in the testis of the rat: studies on its regulation and its importance for the organism. Journal of Nutrition, 112(9):1682-1687.
3
Burk, R.F.; Hill, K.E. and Motley, A.K. (2003) Selenoprotein metabolism and function: evidence for more than one function for selenoprotein P. The Journal of nutrition, 133(5):1517-1520.
4
De Los Monteros, A.E.; Driscoll, S. and Steinke, J. (1970). Insulin release from isolated human fetal pancreatic islets. Science, 168(3935):1111-1112.
5
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41
ORIGINAL_ARTICLE
Seroprevalence of Leptospira interrogans infection in Equids of Lorestan Province: Investigation the role of probable risk factors
Environmental and hosts risk factors are considered as two essential elements in epidemiological studies of infectious diseases. The severity of the infection and also the chance of transmission of pathogenic Leptospira interrogans bacteria from herbivores to humans have always been exposed to environmental and hosts risk factors. This study aimed to investigate the presence of serum reaction in the equid's population of Lorestan province and the determination of native serovars as well as evaluation of the role of environmental and host factors on seroprevalence. A total of 327 equids including 223 horses, 46 mules, and 58 donkeys were sampled and sera samples evaluated against 7 different Leptospira interrogans serovars. Geographical location and environmental conditions of sampled areas were recorded to be used in statistical surveys. The results showed that the serum positive reaction rate in horses, mules, and donkeys was 18.38, 10.87 and 3.45%, respectively. Host risk factors such as sex, age, and breed were effective on serum response rate and a significant difference was found. Canicola serovar with 46.34, 100 and 60% infection in horses, donkeys, and mules, respectively was the most common infective serovar; but a significant difference was not observed between animal species. Also, the results indicated that annual temperature of the environment and altitude at sea level of sampled location have a significant effect on seroprevalence of leptospirosis, but relative humidity and annual rainfall did not have a significant effect. Results of the current study proved that infection with Leptospira interrogans, although in Lorestan province is less severe than in other parts of the country, the most important native serovar of this region is canicola.
https://www.ivj.ir/article_128912_0a1dfe7d4f1ab58dd393e661a01a5a04.pdf
2021-02-19
108
118
10.22055/ivj.2019.156153.2089
Leptospira interrogans
equids
Lorestan
canicola
Zoonosis
shahram
Maleki
shahram.maleki2000@gmail.com
1
Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
LEAD_AUTHOR
Amir
Zakian
amir.zakian7@gmail.com
2
Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
AUTHOR
Gholamreza
Abdollahpour
greza@ut.ac.ir
3
Professor, Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
Adler, B., & Moctezuma, P. A. (2010). Leptospira and Leptospirosis. Journal of Veterinary Microbiology, 140: 287-296.
1
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22
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23
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25
Rezazadeh, F., & Javidi-Brazandeh, M. A. (2010). Age determination and oral and dental diseases in horse. (1st ed). Pardis Bavaran Publication, Tehran, Iran. Pp: 55-101. [In Persian]
26
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27
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28
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29
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30
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31
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32
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33
Ward, M. P. (2002). Seasonality of canine leptospirosis in the United States and Canada and its association with rainfall. Preventive Veterinary Medicine, 56: 203-213.
34
Whitwell, K. E., Blunden, A. S., Miller, J., & Errington, J. (2009). Two cases of equine pregnancy loss associated with Leptospira infection in England. The Veterinary Record, 165: 377-378.
35
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36
ORIGINAL_ARTICLE
The effects of pellet in comparison with mash feed on the production index, intestinal morphology characteristics, index of ascites, quality and microbial count of litter in broiler chickens
This experiment was conducted with the aim of the effects of pellet and mash feed on the production index, intestinal morphology characteristics, index of ascites, quality and microbial count of litter in broiler chickens using three diets of pellet, mash, mash+pellet on broiler performance. This experiment was conducted in a completely randomized design with three treatments and four replicates during 42 days. The measured traits included production index, intestinal morphology traits, ascites, hematocrit percentage, litter moisture and nitrogen content and microbial count of litter. In the whole of the total period, the results showed that the highest production indexes were pellet and pellet+mash treatments. The results of the morphological traits of the intestine showed that pellet and pellet+mash treatments had the highest ratio of villus height to the crypt depth in jejunum and the lowest level of mash treatment was observed. The results showed that the high ratio of villus height to the crypt depth in jejunum resulting from the use of pellet and pellet+mash ration due to production index was improved. The consumption of mash and pellet+mash treatments in the first three weeks reduced ascites significantly and increased with the prolonged feeding period of the pellet diet. The pellet+mash and all-flour treatments had the lowest amount of moisture content and nitrogen content, and as a result, the microbial count of litter was lower than that of pellet treatment. The pellet+mash treatment had the lowest index of ascites (32.62%) and less hematocrit (36%). The final result was that the use of pellet+mash treatment had the highest production index and the most suitable litter quality and the lowest ascites and microbial count compared to the full pellet diet in broiler chickens.
https://www.ivj.ir/article_128914_eac6843dd0cb019b1d96e342ce136c41.pdf
2021-02-19
119
132
10.22055/ivj.2019.170684.2117
Pellet and mash feed
production
intestine morphology
ascites
broiler
Behrouz
Yarahmadi
behrouzy@gmail.com
1
Assistant Professor, Department of Animal Sciences, Lorestan Agricultural and Natural Resources Research and Education Center, AREEO, Khorram Abad, Iran
LEAD_AUTHOR
mohsen
mohamadi saei
mohsenmohamadi57@gmail.com
2
PhD of Animal Science, Department of Animal Sciences, Lorestan Agricultural and Natural Resources Research and Education Center, AREEO, Khorram Abad, Iran
AUTHOR
Akbar
Yaghoobfar
yaghobfar@yahoo.com
3
Professor, Department of Animal Nutrition, Animal Sciences Research Institute of Iran, AREEO, Karaj, Iran
AUTHOR
Hassan
Norouzian
noroozianh@gmail.com
4
Assistant Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorram Abad, Iran
AUTHOR
Al-Homidan, A., Robertson, J. F. &Petchey, A. M. (2003). Review of the effect of ammonia and dust concentrations on broiler performance. Worlds Poultry Science Journal, 59, 340-349.
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2
Amouian Khaveh, A., Karim Tarshizi M. A. &Shariatmadari, F. (2016). Performance, susceptibility to ascites, and economic efficiency in broiler chickens under the influence of alternate feeding program mesh- pellet. Journal of Animal production, 18(1), 85-94. (In Persian).
3
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5
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7
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