@article { author = {Azarkamand, Behzad and Pourmahdi Borujeni, Mehdi and Gharibi, Daryoush and Ghorbanpour, Masoud}, title = {Comparison of serological methods for the diagnosis of brucellosis in water buffalo}, journal = {Iranian Veterinary Journal}, volume = {13}, number = {1}, pages = {5-12}, year = {2017}, publisher = {Shahid Chamran University of Ahvaz}, issn = {1735-6873}, eissn = {2676-704X}, doi = {10.22055/ivj.2017.36718.1612}, abstract = {Brucellosis is zoonotic and contagious disease caused by the genus Brucella. In buffalo, the disease mainly occurred by Brucella abortus and Brucella melitensis and causes significant economic losses including abortion, loss in milk production and low fertility rates in animals. In this study, serum samples from 320 slaughtered water buffaloes were collected from Ahvaz abattoir during 2014-2015. The collected sera were analyzed for the presence of antibody against brucella by two commercial ELISA kits (IDEXX and ID vet), Wright and Rose Bengal methods and the results were analyzed by Cochran and Mc Nemar test. The results showed that the seroprevalence of brucellosis with Wright method was 1.56%. Significant difference was seen between IDEXX (P<0.001) and ID vet (P<0.01) ELISA kits with Wright and Rose Bengal methods, but there was no significant difference between two ELISA kits (P>0.05). Agreement between Rose Bengal and Wright methods with IDEXX and ID vet ELISA kits was moderate and Kappa statistic for these associations were 0.43 and 0.48 respectively, but agreement between Rose Bengal method with Wright method was very good and Kappa statistic for these association was 1. Agreement between IDEXX and ID vet ELISA kits was very good and Kappa statistic for this association was 0.86. This research showed that the sensitivity of ELISA is more than Rose Bengal and Wright methods, but according to the strategy of disease control in Iran, it is recommended that this method is designed for practical use by determining the cut-off point of the test.}, keywords = {Brucellosis,Diagnosis,Serology,buffalo}, title_fa = {مقایسه روش های سرولوژیک تشخیص بروسلوز در گاومیش}, abstract_fa = {بروسلوز بیماری مسری و مشترک بین انسان و دام است که توسط باکتری‌های جنس بروسلا ایجاد می-شود. این بیماری در گاومیش عمدتا توسط بروسلا ابورتوس و بروسلا ملی‌تنسیس ایجاد می‌شود و سبب خسارت‌های اقتصادی مانند سقط، کاهش تولید شیر و کاهش باروری در حیوانات می‌شود. در این تحقیق، با مراجعه تدریجی به کشتارگاه اهواز طی سال‌های 1394-1393 نمونه‌ی سرم 320 رأس گاومیش کشتار شده در کشتارگاه اهواز جمع‌آوری گردید. حضور آنتی‌بادی‌های ضد بروسلا در نمونه‌های سرمی جمع‌آوری شده به وسیله‌ دو کیت الیزای تجاری ( IDEXX و ID vet) و روش‌های رایت و رزبنگال مورد بررسی قرار گرفت و نتایج حاصل با استفاده از آزمون کوکران و مک‌نمار مورد تجزیه و تحلیل قرار گرفت. نتایج نشان داد که شیوع سرمی بروسلوز در روش رایت 56/1 درصد است. تفاوت معنی-داری بین کیت الیزای IDEXX (001/0>P) و ID vet (01/0>P) با روش رزبنگال و رایت وجود داشت، اما دو کیت الیزا تفاوتی نداشتند (05/0