Genetic variation in exon 2 of GDF9 gene in two breeds of goats from Khuzestan
Elham
Javdan
دانشگاه کشاورزی و منابع طبیعی رامین خوزستان
author
جمال
فیاضی
گروه علوم دامی دانشگاه کشاورزی و منابع طبیعی رامین خوزستان
author
محمدتقی
بیگی نصیری
گروه علوم دامی دانشگاه کشاورزی و منابع طبیعی رامین خوزستان
author
صالح
طباطبایی وکیلی
گروه علوم دامی دانشگاه کشاورزی و منابع طبیعی رامین خوزستان
author
text
article
2014
per
Litter size is an important economical trait in sheep and goat breeding. Applying traditional animal breeding systems such as selection and mating based on phenotypic evaluations are time-consuming and usually costly. In this case, molecular genetics can be an alternative solution. Some genetics studies have proven that litter size is genetically determined by the action of single genes with a major effect, named fecundity genes. This study was carried out to detect polymorphism in GDF9 gene. Ninety one Najdi goats of 3 locations in northwest, southeast and center of Khouzestan and 27 Lori-Bakhtiari goats, in 2 locations, north-eastern and south-eastern of province were selected randomly. After extracting of DNA, amplification of a 295 bp fragment of exon 2 of GDF9 gene was performed using specific primers. In this research three SSCP patterns for exon 2 of GDF9 gene in two breeds of goats were found in Khuzestan Province. However, no significant correlation between the observed patterns and the multiple pregnancy rate was observed in the two goat breeds. Allele A in Najdi goat populations of the northwest and southeast, and in Lori-Bakhtiari goats of northeast and southeast have the highest frequency. AB genotype had the highest frequency in Najdi goats of southeast and middle populations while, in the northwest population, the AA genotype was more frequent. The populations of Lori-Bakhtiari goats in all studied locations (northeast and southeast) exhibit the AA genotype as the most frequent genotype.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
2
no.
2014
5
11
https://www.ivj.ir/article_5831_9c7c9bec7336b606dc671d6772d23e06.pdf
Morphology of red blood cells in tetraploid rainbow trout, Oncorhynchus mykiss larvae
سالار
درافشان
گروه شیلات، دانشکده منابع طبیعی، دانشگاه صنعتی اصفهان
author
امیر
وفایی سعدی
گروه شیلات، دانشکده منابع طبیعی، دانشگاه صنعتی اصفهان
author
علی
نکویی فرد
موسسه تحقیقات علوم شیلاتی کشور، مرکز تحقیقات ژنتیک و اصلاح نژاد ماهیان سردآبی شهید مطهری یاسوج و مرکز مرجع آرتیما در منطقه میانی و غربی آسیا (اورمیه).
author
text
article
2014
per
The aim of this study was to evaluate some red blood cell abnormalities in diploid and tetraploid rainbow trout. Tetraploidy induction was done using heat shock 28°C for 10 min, starting at 65 degree-hour post fertilization on fertilized eggs from Iranian brood stock. Eggs from the same lot, without heatshock treatment were selected as control (diploid) group. Ploidy status was verified using red blood cell dimensions as well as the number of nucleular organizing regions in the cell. The results showed that tetraploidy increased all red blood cell dimensions in comparison to diploids (P<0.05). Red blood cell abnormalities were significantly higher in tetraploids compared to diploids (P<0.05). The most frequent cell abnormalities were segmentation of the nucleus, 8.2%; amitosis, 4.1%; immature cell 3.8% and cell with incomplete membrane about 1%. The imbalanced increase in red blood cell dimensions plus significant elevation in cell abnormalities would affect the tetraploid fish welfare and fitness in comparison to diploids.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
2
no.
2014
12
18
https://www.ivj.ir/article_5832_c1fd832c36b4c536605a0b525829d0a0.pdf
Evaluation of potential sensitization of Morganella morganii to acidic conditions by Ethanol and EDTA
مهدی
زارعی
author
مهدی
پورمهدی بروجنی
دانشگاه شهید چمران اهواز
author
سید علی
جزایری
دانشگاه شهید چمران اهواز
author
text
article
2014
per
The ability of bacteria to tolerate low pH is a very important trait to survive in a variety of environments. Morganella morganii, the most prolific histamine former in fish and seafood products, has the ability to tolerate low pH and survive in acidic environments. In the present study, the ability of EDTA and ethanol to sensitize M. morganii to low pH and organic acids was assessed. To achieve this purpose, cells of M. morgani in exponential or stationary growth phases were exposed to pH=5, adjusted by adding hydrochloric, acetic, lactic, citric or tartaric acids into TSB, in the presence of 5 % ethanol and/or 7.5 mM EDTA, for one hour. Survival percentage was obtained by dividing the surviving population by the initial population. According to the results of the present study, 5 % ethanol made both the exponential and stationary phase cells of M. morganii more sensitive to acidic environments tested (p<0.05). The same results were observed for 7.5 mM EDTA. Combination of 5 % ethanol and 7.5 mM EDTA in acidic environments showed more pronounced effects on the acid tolerance of M. morganii (p<0.05), while in the presence of this combination the lowest survival percentages of exponential and stationary phase cells of the bacterium were observed. Thus, ethanol and EDTA, individually or combined, can enhance the rate of inactivation of M. morganii during exposure to low pH and organic acids. Furthermore, in the present study, exponential phase cells of M. morganii were more susceptible to acidic conditions than the stationary phase cells (p<0.05).
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
2
no.
2014
19
28
https://www.ivj.ir/article_5833_edf0b970559e15692092df051fe68b73.pdf
Study of the distribution of infectious pancreatic necrosis (IPN) in rainbow trout farms of Iran using RT-PCR
مهدی
سلطانی
گروه بهداشت و بیماری های آبزیان، دانشکده دامپزشکی دانشگاه تهران
author
شقایق
روح الهی
دانشجوی دکتری تخصصی بهداشت و بیماری های آبزیان، دانشکده دامپزشکی، دانشگاه تهران
author
اشکان
زرگر
استادیار گروه بهداشت و بیماری های آبزیان، دانشکده دامپزشکی دانشگاه تهران
author
کاظم
عبدی
متخصص بهداشت و بیماری آبزیان، دفتر بررسی و مدیریت بیماری های آبزیان، سازمان دامپزشکی کشور
author
سمیرا
محمدیان
دانشجوی دکتری تخصصی بهداشت و بیماری های آبزیان، دانشکده دامپزشکی، دانشگاه تهران
author
امرا...
قاجاری
دستیار پاتولوژی دامپزشکی، دفتر بررسی و مدیریت بیماری های آبزیان، سازمان دامپزشکی کشور
author
text
article
2014
per
In this case-study, geographical distribution of infectious pancreatic necrosis (IPN) was studied in 18 provinces of Iran using RT-PCR during 2011-2012. Since the occurrence of coinfection by IPNV, infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicaemia virus (VHSV) with the same clinical signs can occur, all samples were tested for presence of these three virus agents. Samples of larva and juveniles from rainbow trout with clinical signs such as anorexia, exophthalmia; darkened skin and high mortality as well as sperms and oval samples were obtained. Results showed that from 214 pooled samples, 96 (44.85%) in 15 provinces were positive for IPN. The highest relative frequency was observed in Lorestan, Isfahan, Chaharmahal va Bakhtiyari, Fars, Kermanshah, Kohgilouye va Boyerahmad, Mazandaran and Qazvin. These results show that IPN disease is distributed in north, western north, west, western south and south parts of country. Therefore it is important to improve the preventive criteria to decrease the economical losses due to the disease morbidity and mortality in farmed trout in Iran.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
2
no.
2014
29
39
https://www.ivj.ir/article_5834_34814c02df52e473a9531127fb40e3f7.pdf
Effect of dietary supplementation Spirulina platensis on blood, immunological and serum biochemical parameters of Benny fish Mesopotamichthys sharpeyi (Günther, 1874)
Reza
Saligheh Zadeh
Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr Marine Science and Technology
author
Vahid
Yavari
Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology
author
Seyed Mohammad
Mousavi
Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology
author
Mohammad
Zakeri
Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology
author
text
article
2014
per
Effect of different levels of spirulina dietary supplementation on some blood-immune parameters and serum biochemical parameters of Benny Mesopotamichthys sharpeyi fingerlings were investigated in an 8 weeks experimental period. Dried and powdered spirulina algae in five levels of 0, 2.5, 5.0, 7.5 and 10.0 % was added to the experimental diet. Experimental fish were fed to satiation twice daily. Haemato-immunological and blood serum biochemical parameters were higher in benny fish receiving diet with 10% spirulina compared to fish being fed diet with 0% of spirulina. Haematological parameters and blood serum biochemical parameters including haemoglobin concentration, haematocrit, total protein, total albumin, total globulin and albumin/globulin ratio were not significantly (P>0.05) affected by spirulina supplementation. However, lysozyme activity levels in 10% treatment were higher and showed a significant difference (P<0.05) from control treatment. The results revealed that spirulina supplementation at 10% level leads to improve haemato-immunological and serum biochemical parameters of benny fish.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
2
no.
2014
40
46
https://www.ivj.ir/article_5835_9da7ee48545f36d82c7120c206d7effa.pdf
Histologغl and micrometقغ of Gut Associated Lymphatic Tissue (GALT) in mature and immture Silver carp fish
Naeem
Erfanmi Majd
نویسنده مقاله
author
رحیم
پیغان
هیات علمی
author
زینب
یعقوبی
دانشجو
author
text
article
2014
per
Gut Associated Lymphoid tissue (GALT) is a primary lymphoid tissue in mammals and it is a first defense barrier for pathogen factors. There are many reports about existence of inter and intra species differences in fish GALT structure. In this study 10 Silver fish (5 mature and 5 immature) were selected and 0.5 cm samples were taken from intestine bulb to terminal part of main intestine (colon). The 5-6 µm sections were made using paraffin embedding method. The sections were stained by H&E and PAS. The results showed that the GALT are located in 3 regions and in 2 types. The first, distributed in epithelial tissue, the second location was in lamina propria as columnar or aggregated shapes, and the third location was in submucosa. The lymphoid tissue is consisted mainly from lymphocytes, lymphoblasts, a few plasma cells and macrophages. There is a significant difference in intraepithelial lymphocytes number in mature and immature fish (P<0.05). The maximum lymphocytes were seen in intestinal bulb and main intestine of both mature and immature fish. The results showed that there are significant differences in location, shape and distribution of lymphoid tissue in silver carp fish.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
2
no.
2014
47
56
https://www.ivj.ir/article_5836_4b66602e71f4ea70069a977f76fe2806.pdf
Ultrasonographic Study of the Reticulum in Healthy Cow
Alireza
Ghadiri
عضو هیات علمی
author
Alireza
Mashahadi
هیات علمی دانشگاه شهید چمران
author
هادی
نداف
هیات علمی دانشگاه شهید چمران
author
امید
ممبینی
اداره دامپزشکی هفتکل
author
text
article
2014
per
The main aims of this study were to obtain technique and to present ultrasonographic findings of reticulum and adjacent organs of native and mixed breed healthy cows. The reticulum and some adjacent organs were examined ultrasonographically in 15 healthy native and mixed breed cattle. Ultrasonography was performed on standing, nonsedated cattle, with linear and convex transducers. Transducer was applied to the ventral aspect of abdominal cavity, caudal to the xyphoid and the most ventral part of both thoracic walls over the sixth and seventh intercostals spaces. Evaluation included assessment of the contour of the reticulum, reticular contractions, and the organs adjacent to the reticulum. The normal reticulum appeared as a half-circle structure with a smooth contour and a hyperechoic wall; it contracted at regular intervals and was situated immediately adjacent to the ventral portion of the abdominal wall when relaxed. Contents of the reticulum could not be imaged because of its partly gaseous composition. The ruminoreticular groove, craniodorsal blind sac of the rumen, and the ventral sac of the rumen were observed caudal to the reticulum. The distal aspect of the spleen and parts of the abomasum could be imaged. Reticular motility was characterized by a bi-phasic contraction pattern. Four to five biphasic reticular contractions usually were observed during a 4 minutes period. The first reticular contraction lasted a mean (± SD) of 2.69 (±0.30) seconds and the second contraction lasted 4.05 (±0.55) seconds. The mean interval between 2 biphasic contractions was 46.2 (±4.5) seconds. Based on our findings, ultrasonography is useful for assessing the reticulum.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
2
no.
2014
57
64
https://www.ivj.ir/article_5837_a0f5e88b8f6e450e35b0746270032f1d.pdf
Survey on Tetracycline and Oxytetracycline antibiotic residues in honeys produced in some honey hives in Khuzestan province using HPLC method in 2012
Ali
Fazlara
دانشیار گروه بهداشت مواد غذایی
author
Hossein
Najafzadeh Varzi
دانشگاه شهید چمران اهواز
author
bahareh
Izadi
Graduated from Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz,
author
text
article
2014
per
Tetracyclines have been used for treatment of European and American foulbrood in beekeeping since years ago. Lack of attention to veterinary recommendations by beekeepers about time and dosage of antibiotics consumption leads to increase antibiotics residues in honey more than standard or Maximum Residual Limits (MRL). In attention to the side effects of antibiotic residues like drug resistance, consumers hyperesthesia etc., the investigation for tetracycline and oxytetracycline residues was considered in some consumed honey samples in Khuzestan province by HPLC method. Totally 60 honey samples were collected from Khuzestan beekeepers and processed based on the protocol for investigation of tetracycline and oxytetracycline residues. According to this matter and in order to extract antibiotic residues, 5 g of each honey sample was mixed and dissolved with 25 ml of McIlvaine buffer and filtered. Then after calibration of HPLC system, 20 µl of each solution was injected to HPLC system and finally the amounts of tetracycline and oxytetracycline residues were determined for each sample. This study showed that 14 (23.33%) samples of whole investigated honeys had no antibiotic residues while 31 (51.66%) out of 60 collected honey samples contaminated to both tetracycline and oxytetracycline residues. The means of tetracycline and oxytetracycline residues in positive samples were equal to 119.23±31.38 and 167.636±37.02 µg/kg respectively. In conclusion, in present study 46 (76.67%) out of 60 studied honey samples contained antibiotic residues. These results showed significant difference with zero µg/kg limits as MRL determined by European Union, Codex Alimentarius and Food and Drug Administration (p≤0.05).
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
2
no.
2014
65
73
https://www.ivj.ir/article_5838_19d1d6b7696940fefe948615240ac247.pdf
Detection of Chlamydophila abortus in sheep abortions in Chaharmahal va Bakhtiari Province using Nested PCR
Mohammadreza
Mahzounieh
Faculty member, Shahrekord University
author
شبنم
گلبوی داغداری
دانشجوی کارشناسی ارشد باکتری شناسی
author
Razieh
Pourahmad
عضو هیئت علمی/ گروه ژنتیک/ دانشکده علوم پایه/ دانشگاه شهرکرد
author
text
article
2014
per
Chlamydophila abortus, the aetiological agent of enzootic abortion of ewes (EAE) is a major cause of lamb loss in many sheep-rearing countries throughout the world. Usually the first clinical manifestation of disease is abortion in the last 2–3 weeks of gestation or when the ewe gives birth to stillborn or weak lambs. Because Chlamydophila does not grow in vitro, organisms usually are not found by routine diagnostic tests. Therefore, it is recommended to detect antigen or genome. The aim of this study was to investigate the presence of these bacteria in aborted sheep fetuses in Chaharmahal va Bakhtiari province. Samples included 48 aspirated liquid from abomasums of aborted fetuses during two lambing seasons, 2011- 2012. The samples were tested by Nested PCR to identify specific 16S rRNA sequence. In this study, EAE accounted for around 52% of all diagnosed abortions. It suggests that C.abortus was a main abortion agent in tested samples and caused considerable reproductive losses particularly in areas where flocks were kept closely congregated during lambing season. Therefore, due to the severe economic losses and the risk of human infection, more research should be planned and done for prevention and control of C.aborus infection.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
2
no.
2014
74
80
https://www.ivj.ir/article_5839_5c8bfb69ea8fd722722b8c4a565b84d3.pdf
Comparison between male and female rabbit renal arteries by Doppler ultrasonography
abdolvahed
moarabi
shahid chamran university
author
Reza
Avizeh
نویسنده دوم
author
محمد قاسم
حنفی
نویسنده سوم
author
مهدی
پور مهدی بروجنی
نویسنده چهارم
author
لنا
مهدیه دولت آبادی
نویسنده پنجم
author
text
article
2014
per
Doppler ultrasonography is important in diagnosis of renal diseases by demonstrating vascular anatomy and hemodynamics. Decreased renal blood flow may be the first sign of dysfunction and can be evaluated by doppler ultrasound. The purpose of this study was to obtain the normal images of color doppler and estimation of blood flow velocity parameters of renal arteries and abdominal aorta in adult healthy, New Zealand white rabbits. Therefore, 20 healthy rabbits (10 male and 10 female) with a mean age of 15 months (12 to 21 months) and a mean weight of 1.7 kg (1.4 to 2.6 kg) were selected. In this study, indices of abdominal aorta and renal arteries including PSV, EDV, RI, PI, MV, VF and pulsed Doppler spectral were compared between the sexes. Mean PSV and EDV of abdominal aorta in male and female rabbits were 68.95 and 76.19cm/sec respectively. Mean PSV and EDV of renal artery in males and females were 42.12 and 30.95cm/sec respectively. Numerical values obtained for the PSV, MV and VF of the abdominal aorta and EDV and VF renal arteries between males and females were different (P<0.05). The results obtained in this study can be used as indicators of natural indices in diagnosis of associated diseases in New Zealand white rabbits.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
2
no.
2014
81
87
https://www.ivj.ir/article_5840_054c16407242d7a1436224990a4dd44a.pdf
Isolation and Biochemical Identification of Potentially Probiotic Bacteria from Barbus grypus intestine
تکاور
محمدیان
دانشجو
author
takavar
mohammadiyan
عضو هیات علمی دانشگاه شهید چمران اهواز
author
مجتبی
علیشاهی
عضو هیات علمی دانشگاه شهید چمران اهواز
author
M
Tabandeh
author
D
Gharibi
author
text
article
2014
per
Lactic acid bacteria (LAB) are the most common type of microbes used as probiotics. Isolation and identification of lactic acid bacteria (LAB) from Barbus grypus intestine, based on their probiotic effects, were aimed in this study. Fifty B.grypus (800.82±265.97) were captured from natural water resources of Khuzestan province and transferred to laboratory. The bacterial flora of fish intestine were assessed. The intestinal LABs isolated and identified according to biochemical and morphometrical tests. Isolated LABs were evaluated and ranked based on probiotical indication tests (acid resistance, bile salt resistance , bacterial antagonistic effects and lack of pathogencity in fish). A total of thirty bacillus or cocci shape bacteria, suspected LABs, were isolated. Ten isolates identified as Lactobacillus and more identified to species based on biochemical tests. The probiotical identities of these ten isolates compared and finally four respectively isolates including L. acidophilus، L. delbruecki L.brevis and L. fermentium, were selected as potentially probiotic LABs of B.grypus intestine.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
2
no.
2014
88
97
https://www.ivj.ir/article_5841_2819bf18bb0c09ee98df417c9bf3c020.pdf
Occurrence of viral thymic lymphoma associated with generalized lymphadenopalty and splenomegally in a 3.5 month old Holstein female calf
تقی
تقی پور بازرگانی
استاد دانشگاه تهران
author
Mohammad Javad
Gharagozlou
professor of University of Tehran
author
فرهاد
موسی خانی
بخش خصوصی
author
سعید
مهدی زاده
بخش خصوصی
author
text
article
2014
per
In a dairy farm with 1600 milking cows, a 3.5- month old Holstein female calf showed a prominent hard subcutaneous mass at the lower part of the neck resulted in rumen bloat and dyspnea. In postmortem examination of the calf, apart from huge mass of thymus, astonishing enlargement of innumerable lymph nodes and a large spleen were noticed as well. In histopathologic study, replacement of the thymus, lymph nodes and spleen tissues with immature malignant lymphocytes were found.The adjacent muscles and fats of the extra-thoracic thymus lymphoma were invaded by the neoplastic lymphoid cells. CBC of the patient showed leukocytosis (more than 45000) and lymphocytosis (higher than 93% with 2% atypical lymphocyte). PCR of blood sample of the calf was positive and in ELISA test, the serum antibody was negative (28pp). PCR and ELISA of the mother of this calf were negative while PCR of a sample from the dairy milk tank was strongly positive. It is suggested that due to lack of maternal passive immunity and immaturity of immune system, following ingestion of BLV- contaminated milk, the calf became infected, resulted in occurrence of atypical and widespread viral lymphoma..By the best knowledge of the authors this is the first report of this kind of calf lymphomsarcoma world-wide.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
2
no.
2014
98
103
https://www.ivj.ir/article_5842_5ca4fba6644a559d3c0e137da97e48fb.pdf