Study effect of silymarin on abdominal adhesions after entrotomy in dog
علی
بنی آدم
عضو هیات علمی- دانشگاه شهید چمران اهواز
author
سایه
صمیمی
دانش اموخته دکتری دامپزشکی
author
حسین
نجف زاده
دانشگاه شهید چمران اهواز
author
text
article
2014
per
The objective of this study was to show the effect of silymarin on peritoneal adhesions in dog. Ten adult male and female dogs were divided into two equal groups randomly: control and intraperitoneal silymarin. A 10-cm midline laparotomy incision was made in all dogs. After entrotomy, the abdominal wall incision was sutured. Re-laparotomy was performed on the 30th postoperative day and adhesion formation was macroscopically graded according to the Nair classification and Adachi classification. Serum malondialdehyde and total antioxidant activity were determined. Adhesion scores in silymarin group were significantly (p<0.05) greater than control group. The adhesion formation in silymarin group was more extensive and denser than control group. Silymarin might not be absorbed from the peritoneal cavity and act as a foreign substance. The serum concentration of malondialdehyde and total antioxidant activity was not different between control and silymarin groups significantly. Thus, silymarin does not have any benefitial effect on peritoneal adhesions, in local administration.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
3
no.
2014
5
12
https://www.ivj.ir/article_6371_5160a90ea4c788c11b4ead9a2450992b.pdf
Characterization of trap types in Staphylococcus aureus isolates from mastitis bovine and ovine milk samples in the North West of Iran
Habib
Dastmalchi Saei
Assistant Professor, Department of Microbiology, Faculty of Veterinary Medicine, Urmia University
author
Somayyeh
Hosseinzadeh
دانشجوی PhD باکتریشناسی، دانشکده دامپزشکی، دانشگاه ارومیه
author
text
article
2014
per
The virulence of Staphylococcus aureus is essentially determined by cell wall associated proteins and secreted toxins, whose expression is largely regulated by two-component regulatory systems (TCRSs) such as accessory gene regulator (agr) and RAP-TRAP systems. Four allelic variants of agr (agr I-IV) and trap (trap 1-4) so far have been identified. Some reports state that there are clinical trends according to each agr group, but there is a scarcity of information about trap types. So in this study, the polymorphism of the trap locus was analyzed by restriction endonuclease PCR in a population of 43 epidemiologically unrelated S. aureus isolated from bovine (n=21) and ovine (n=22) mastitis. The entire 504-bp open reading frame of the trap gene was amplified from all isolates by PCR. The PCR products were then digested with MseI, giving two different profiles corresponding to trap types 2 and 3. Isolates from cow were classified in trap types 2 (13; 61.9%) and 3 (8; 38.1%). The vast majority of isolates recovered from sheep belonged to trap type 2 (90.9%) and type 3 contained only 9.1% of the sheep isolates. In general, types 2 and 3 accounted for 76.7% and 23.3% of the analyzed isolates, respectively. It is worth noting that trap types 1 and 4 were not detected among the studied isolates. In conclusion some trap types were highly prevalent in mastitis cases probably because of possession of a genetic background which endows them with superior ability to infect the mammary gland.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
3
no.
2014
13
20
https://www.ivj.ir/article_6372_11b6a160fde2a8ada592b0c29f8e50ab.pdf
Study on Prevalence of Monogenean trematodes in some Cyprinid species of Gamasiab River ,Hamedan Province
Javad
Daghigh Roohi
Head of Aquatic Animals Nutrition and Live Food Research Station
author
Behyar
Jalali Jafari
Professor of veterinary faculty / Azad University, Science and Research Branch
author
Hasan
Nezamabadi
Fish health and disease department/ Iranian Fisheries Research Organization
author
Gholamreza
Mehdizadeh
MSc. in National Inland Water Aquaculture Reaserch Institute
author
text
article
2014
per
Gamasiab is the constant and most important river of Nahavand plain in Hamedan province and one of the longest rivers of Iran. In order to investigate the aquaculture potential of this region, a survey of natural fish parasites of this river were done by two seasonal sampling at summer and autumn in 2008. Totally 169 pieces of 7 Cyprinid species were investigated and 5 different species of monogenean parasites isolated from skin and gills of the fishes. Dactylogyrus lenkorani was isolated from branches of Capoeta damascina, Barbus lacerta, Capoeta aculeate and Capoeta trutta with infection percentage of 60%, 100%, 66.6% and 50%, respectively. Dactylogyrus vistulae was isolated from branches and Gyrodactylus sp. from skin of Squalius cephalus with infection percentage of 40% and 7.2%, respectively. Dactylogyrus holciki segregated of Alburnus mossulensis branches by about 5.8% infection. Finally Paradiplozoon sp. was isolated from gills of Chondrostoma regium and Barbus lacerta with 9.5% and 10% infection, respectively. This survey introduces a part of parasitic fauna of Gamasiab river in Hamedan province. Infection of Barbus lacerta with D. lenkorani was reported for the first time from Iran.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
3
no.
2014
21
28
https://www.ivj.ir/article_6373_32af3d9b118f6f0d86649c429cf591a0.pdf
Effect of dietary ascorbic acid on bone collagen and body composition of the Caspian brown trout (Salmo trutta caspius) fingerlings
Houman
Rajabi Islami
Assistan Professor in Department of Fisheries
author
Narges
Arab
M.Sc. Graduated of Fisheries
author
text
article
2014
per
Ascorbic acid (AA) is known to perform numerous biochemical and physiological functions especially for teleost fish. A feeding trial with a total of six hundred Caspian brown trout fingerlings (9.6±0.6 g) was conducted to determine the effect of AA on bone collagen and body composition of the Caspian brown trout (Salmo trutta caspius) as a valuable native teleost species. Five different diets containing 0, 50, 100, 200 and 400 mg kg-1 L-Ascorbyl-2-Polyphosphate were prepared as experimental treatments. Feeding of the fish was done in triplicates for 9 weeks in each treatment, each containing 40 Caspian brown trout fingerlings. The results showed significant differences in final weight, bone collagen and body composition of Caspian brown Trout fingerlings at the end of the feeding trial (p<0.05). Increase trends in AA amount of diets cause significant rise of weight (p<0.05), reaching to the final weight of 29.74±3.52 g at the 200 mg AA kg-1 treatment. Fish fed by 200 mg AA kg-1 diet had also the maximum amount of bone collagen; which had significant difference with control treatment (p<0.05). Besides, the highest ash content and fat concentrations were found in 50 mg kg-1 AA. Based on the broken line model, the optimum bone collagen and protein amounts were obtained at 247.54 and 250.55 mg AA kg-1 diet, respectively.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
3
no.
2014
29
42
https://www.ivj.ir/article_6374_5f374fe9e2905cfd40d7e8f841ce9e94.pdf
The effects of citric acid and two medicinal plants fennel and fenugreek on growth performance, Humoral immunity, serum protein and intestinal microbial population in broilers
جلال
سالاری
مدرس دانشگاه علمی کاربردی قم
author
مجید
کلانتر نیستانکی
عضو هیئت علمی مرکز تحقیقات کشاورزی و منابع طبیعی قم
author
فاطمه
صاحبی اعلا
دانشگاه بوعلی سینا
author
text
article
2014
per
This experiment was conducted to investigate the effects of citric acid and medicinal plants on growth performance, humeral immunity, serum proteins and intestinal microbial population in broilers. A total number of 225 Ross 308 (one-day old) broiler chickens were randomly divided into 4 treatments with 6 replicates of 3 chicks each. Treatments including: 1- basal diet (without any additives), 2- basal diet + medicinal plants in equal mounts (0.5%), 3- basal diet +medicinal plants in equal mounts (0.5%) + 0.5% citric acid, 4- basal diet + 0.5 % fenugreek + 0.5 % citric acid, 5- basal diet + 0.5% fennel + 0.5% citric acid. Feed intake was significantly higher in treatment 5 compared to control and FCR was significantly higher in treatment 5 and 2 compared to treatment 3 and 4 (P< 0.05). Total serum protein levels were higher in treatment 2 than in treatment 1 (P< 0.05). Albumin / globulin ratio was significantly higher in treatment 3 and 4 compared to other treatments. The results indicated that the overall use of fennel and fenugreek plants and citric acid had satisfactory effects on growth and performance of broiler chickens.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
3
no.
2014
43
48
https://www.ivj.ir/article_6375_0c9315bb167941c62a8c71f6214d8318.pdf
A survey of Ovine Herpesvirus-2 (OvHV-2) infection among Ahvaz sheep population by PCR
مسعودرضا
صیفی آبادشاپوری
دانشگاه شهید چمران اهواز
author
محمد
رشنو
دانشجو
author
ساوش
منصوری
دانش آموخته
author
محمد
صباغان
داشجو داشنگاه علوم پزشکی جندی شاپور
author
مجتبی
حقی کرم اله
دانشجو
author
ماجده
بلادی موسوی
دانش آموخته
author
رضا
دهنوی زاده کازرونی
دانش آموخته
author
text
article
2014
per
Malignant catarrhal fever (MCF) is one of the most important viral diseases of cattle and some other animal species. MCF in cattle is due to infection by two viruses: alcelaphineherpesvirus-1 (AlHV-1) and ovine herpesvirus-2 (OvHV-2). Epidemiologically, MCF in cattle is associated with wildebeests (natural host of AlHV-1) or sheep (natural host of OvHV-2). The aim of this study was to investigate the OvHV-2 infection in the sheep population of Ahvaz. Therefore, 100 whole blood samples were collected from sheep of the region and tested by PCR. Initially buffy coats were separated from the blood samples and subjected to DNA extraction. Thereafter, the presence of OvHV-2 DNA in the samples was assessed by a semi-nested PCR. The results indicated that 82% of the sampled sheep were carrier of OvHV-2 DNA. Infection rate observed in this study was lower than in some previous studies. This difference could be due to breed differences of sheep or genetic diversity of OvHV-2.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
3
no.
2014
49
54
https://www.ivj.ir/article_6376_a2585056272deece1860a0c44bb159d3.pdf
The preventative role of MgO nanoparticles in amnesia induced by morphine in male mouse
Maryam
Abdolahzadeh dashty
کارشناس ارشد فیزیولوژی ، دانشکده علوم،دانشگاه شهید چمران اهواز
author
Mahnaz
Kesmati
دانشیار گروه زیست شناسی دانشکده علوم دانشگاه شهید چمران اهواز
author
Lotfolah
Khaje por
استادیار گروه زیست شناسی،دانشکده علوم، دانشگاهشهید چمران اهواز
author
Hosein
Najafzadeh varzi
دانشیار گروه فارماکولوژی دانشکده دامپزشکی، دانشگاه شهید چمران اهواز
author
text
article
2014
per
Consumption of opioid agonists such as morphine before and after training distruct the memory. It has been shown that some magnesium supplements improve various forms of memory but influx limitation of the ion via the blood brain barrier has limited its use. Mineral nano particles such as magnesium oxide nano particles (nano MgO) with unique physiochemical properties, can easily pass across biological barriers but their effects on memory and amnesia are not clear. In this study effect of magnesium oxide nanoparticles on morphine induced amnesia has was been investigated. In this study, the mice in the weight range 25to 30g were used in 12 groups consisted: control, receiving morphine (5 and 10 mg/kg), receiving nano MgO (1, 2.5, 5, 10 mg/kg, IP) and interaction groups of morphine and nano MgO. The Step-down apparatus was used for evaluating the passive avoidance memory. The results showed that morphine administration after training in dose of 5 mg/kg made significant amnesia. MgO nanoparticles in doses of 2.5, 5, 10mg/kg after training improved passive avoidance memory but had no effect on locomotor activity. However, mgo in these doses inhibited morphine induced amnesia. It seems that MgO nanoparticles prevent the mechanisms of morphine induced amnesia by passing through blood brain barrier and involving in memory pathways.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
3
no.
2014
55
64
https://www.ivj.ir/article_6377_34a5930b8b4cd5be1e039d2d6ad6ed49.pdf
Distribution and histochemical studies of Goblet mucous cells in different regions of epidermis in mature male and female Silver carp fish
نعیم
عرفانی مجد
هیات علمی
author
مهرزاد
مصباح
iهیات علمی
author
کاوه
اسفندیاری
دانشجو
author
text
article
2014
per
Fish epidermis contains goblet mucous cells which their primary function is to secrete mucous to skin. For histometrical and histochemical studies of these cells, at least 10 mature male and female silver carp fish were selected and 5 mm thickness samples were taken from different parts of skin including head, dorsum, region, venter region and tail. 5-6µ sections were made using paraffin embedding methods, and stained by H&E, PAS, AB (pH=2.5), AB (pH=1) and AB (pH=2.5) PAS. Number of goblet cells were counted in 100 µm length of epidermis in each regions. The results showed that there are small and ellipsoid to polyhedral cells called goblet cells in different regions of silver carp epidermis. These cells have positive reaction to PAS staining which it showing the neutral property of mucous secretion of these cells. The skin of silver carp fish can be divided on the basis of goblet cells distribution in different parts of epidermis (head, dorsal regions, ventral regions and tail). The results revealed that male silver carp fish has maximum goblet cells in head skin but the female has maximum in dorsal skin. The number of these cells in tail part of male and female are relatively same.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
3
no.
2014
65
74
https://www.ivj.ir/article_6378_12cac4fbcece8e448a6dcad46dc1a0bc.pdf
The effects of different concentrations of Acid ascorbic on freezability of Kordi Goat Spermatozoa
seyed mohammad javad
alavi
دانشجو
author
حمید
کهرام
استاد دانشگاه پردیس کشاورزی تهران
author
سعید
زین الدینی
استاد دانشگاه پردیس کشاورزی تهران
author
حمیدرضا
نائیجیان
دانش اموخته
author
text
article
2014
per
This study was designed to investigate the effects of different concentrations of ascorbic acid (0, 2.5, 4.5, 6.5 and 8.5 mg/ml) on freezing of the Kordi goat spermatozoa. Semen samples were collected by an artificial vagina, twice a week from four pubertal goat. The extender containing semen was frozen in liquid nitrogen and then was stored until using for assessment. Semen was thawed at 37◦C and then motility and progressive motility were assessed by CASA. membrane integrity, viability and morphology of sperms were assessed and malondialdehiyde (MDA) concentration was measured. The results of this experiment showed that extender containing 4.5 and 6.5 mg ascorbic acid significantly improved motility (P<0.01), progressive motility (P<0.05) and viability compare to control treatment (P<0.01). Membrane integrity, morphology and malondialdehiyde were not affected significantly by the different level of ascorbic acid (P>0.05). It can be concluded that adding 4.5 mg/ml ascorbic acid in to goat extender is suitable for preservation of sperm and its fertility.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
3
no.
2014
75
82
https://www.ivj.ir/article_6379_7a1535b45ad272cfbbf4d35e96d2d3ab.pdf
Bioaccumulation of waterborne cadmium and its effect on carcass quality of Sterlet (Acipenser ruthenus)
مرضیه
عروجعلی
دانشکده منابع طبیعی، دانشگاه صنعتی اصفهان
author
فاطمه
پیکان حیرتی
گروه شیلات، دانشکده منابع طبیعی، دانشگاه صنعتی اصفهان
author
سالار
درافشان
گروه شیلات، دانشکده منابع طبیعی، دانشگاه صنعتی اصفهان
author
نصراله
محبوبی صوفیانی
دانشکده منابع طبیعی، دانشگاه صنعتی اصفهان
author
text
article
2014
per
The sublethal effects of water - born cadmium (Cd) on carcass quality (moisture, protein, fat and ash contents) and its accumulation in different tissues including gill, kidney, liver and muscle were analyzed. For this purpose, 60 juvenile Sterlet, Acipenser ruthenus, (39.98 ± 0.45 g weight and 22.45 ± 0.65 cm length) were randomly distributed in 4 experimental groups, each containing 3 replicates, with five fish per replicate. The fish were exposed to different concentrations of Cd for 21 days under semi-static experimental condition as follows: 0, 16, 32 and 64 µg/L Cd. At the end of the experiment, the carcass quality (without head and inside body cavity) and Cd accumulation using wet method were determined in different tissues including gills, liver, muscle and kidney in 3 fish from each replicate. The results showed that moisture, protein, fat and ash contents were in the range of 78-81, 12.6-14, 3.5-4.5 and 12.6-14% respectively without any significant differences among groups (P>0.05). By increasing in Cd concentration of the water, the accumulation in all tested tissues (muscle as an exception) was elevated significantly (P<0.05). In all treated groups, the highest accumulation was measured in the kidney, followed by the gills. However, there were no significant differences in Cd accumulation in gills, kidney and liver in any treated group (P>0.05). The only elevation was observed in Cd accumulation in the gill in comparison between the control and fish exposed to 16 µg/l Cd (P<0.05). Based on the results, it could be concluded that although sublethal Cd exposure for 21 day did not affect the carcass quality in juvenile Sterlet, it couldbe accumulated in different tissues (muscle as an exception) in a dose response manner.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
3
no.
2014
83
93
https://www.ivj.ir/article_6380_ec8a1a254fbeeda2215029221fe5298e.pdf
Pool water and Rainbow trout contamination to some enteric bacteria in Chaharmahal va Bakhtiari province
mojtaba
Bonyadian
professor associate of hygiene and food quality control, veterinary college, Shahrekord university, Shahrekord,iran
author
hasan
Fardizad
Veterinary medicine graduated, Islamic azad university shahrekord branch, Shahrekord, iran
author
ava
akbarian
shahrekord university
author
efat
Karimi ghahfarokh
shahrekord university
author
text
article
2014
per
This study was to determine the rate of contamination of water and cold water fish to some enteric bacteria in Chaharmahal va Bakhtiari province. One hundred and eight of fish samples were randomly gathered from fish farms, also 24 water samples obtained from pools separately in twophases. All samples in order to in order to isolate bacteria including salmonellae, Escherichia coli and citrobacter freundii were examined by selective and purified culture media. Results of this study showed that neither of fish nor water samples was contaminated to salmonella, but contamination of fishes to Escherichia coli and citrobacter were 20.3 and 7.4 percent, respectively. Water contamination to Escherichia coli was 66.7 % but there were not any contamination to citrobacter and salmonellae in water samples. As the result of this study showed, Rainbow trout fishes in this area are not considered such as a source for human salmonellosis; however there are some risks that other enteric bacteria such as Escherichia coli may involve people by this food stuff.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
3
no.
2014
94
99
https://www.ivj.ir/article_6381_f3cfe6f00e5387742a4d130e7ab04c94.pdf
The first report of myloencephalopathy caused by equine herpes virus type 1 in Iran
تقی
تقی پور بازرگانی
عضو هیات علمی
author
Hassan
Momtaz
عضو هیات علمی
author
فریدرضا
بهارلو
دامپزشک بخش خصوصی
author
مهدی
غفاری
کارشناس آزمایشگاه
author
text
article
2014
per
The prevalence rate and etiology of EHV-1 and EHV-4 are essentially unknown in Iran. Also, according to the incredible distribution of horse supplies and breeding in recent years in the country and presence of several outbreaks of clinical nervous infections in horses of some areas of Iran, this study for the first time in Iran was carried out in order to isolate of EHV-1 from susceptible cases of myeloencephalopathy in horse clubs of Golpayegan city. In this study the blood samples were collected from three horses of Golpayegan’s horse clubs which had anorexia, depression, ataxia, and recumbence and were suspicious to herpes viral encephalitis. Samples extracted bufly-coat were used for inoculation in allantoic cavity of embryonated eggs and RBK cell culture. Genomic DNA was extracted from both allantoic and cell culture fluids and was evaluated for detection of EHV-1 using PCR method. Results showed the presence of EHV-1 in all three samples.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
10
v.
3
no.
2014
100
103
https://www.ivj.ir/article_6382_59c5febdcdb3ffe2efae1235f36965c4.pdf