Investigation of Polymorphism in 5- Flanking Region of Pit-1 gene and its association with growth traits in Iranian native Goose
Mahsa
Akbari
MSc Graduated of Genetics and Animal Breeding, Faculty of Agriculture, Urmia University, Urmia, Iran
author
Mokhtar
Ghaffari
Assistant Professor, Department of Animal Science, Faculty of Animal Science, Urmia University, Urmia, Iran
author
Ali
Hashemi
Associate Professor, Department of Animal Science, Faculty of Animal Science, Urmia University, Urmia, Iran
author
Ghorban
Elyasi Zarringhabaie
Instructor, East Azarbaijan Agricultural and Natural Resources Research and Education Center, Tabriz, Iran
author
text
article
2018
per
The objective of this study was detection of polymorphism in 5- Flanking region of Pit-1 gene and its association with growth traits in Iranian native Goose by PCR-SSCP method. For this purpose, blood samples were taken from 160 Goose from East Azerbaijan breeding station that located in Malekan. DNA was extracted from whole blood using the Bailes method and polymerase chain reactions were performed for amplification of 241 bp fragment containing in 5- Flanking region of Pit-1 gene.Single Strand Conformation Polymorphism (SSCP) was used for genotyping. For this purpose, vertical electrophoresis of PCR products was performed on 8% acrylamide gel, at 120 V, for 18 h at 4 C˚. Silver-staining of gels is used to detect, resulted four genotypic patterns of 1, 2, 3 and 4 with frequencies of 31.2%, 36.4%, 7% and 25.4%, respectively. Analysis of variance was performed using GLM proc of SAS software. The statistical analysis indicated that the effect of different patterns were significant on the 30-Day Bodyweight (p<0.01), but on the 60-90-150 -Day Bodyweight had no significant effect.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
14
v.
4
no.
2018
5
12
https://www.ivj.ir/article_81729_55ed2017d9f8db0db46855b05b5c7194.pdf
dx.doi.org/10.22055/ivj.2017.73037.1862
Dietary Effect of mulberry (morus alba) leaves supplementation on intestinal villi morphology of broiler chickens.
Seyed Saatar
Tohidifar
DVSc Graduated of Poultry Science, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
author
Shahab
Bahadoran
Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
author
Hosein
Hasanpour
Associate Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
author
Mohammad Hasan
Mehraei Hamzekolaei
DVSc Graduated of Poultry Science, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
author
Elahe
Askari
Assistant Professor, Department of Nutrition, Faculty of Health and Nutrition, Lorestan University of Medical Sciences, Khorramabad, Iran
author
Javad
Gholamzadeh
DVM Graduated from Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
author
text
article
2018
per
To investigate the effect of dietary Morus alba leaves on intestinal morphology of broiler chickens, 180 one day old Ross chicks were purchased and randomly divided into four equal groups (each group contains 45 chickens in 3 replicates). Group 1 as control group received basal diet, groups 2, 3 and 4 received diet supplemented with 0.25%, 0.5% and 1% Morus alba leaves, respectively. Chicks were provided a standard diet base on corn. Chicks were raised on floor-pen under standard conditions until day 42 of age. At days 21 and 42, six chicks of each group were randomly selected and samples were taken from Duodenum, Jejunum and Ileum for morphometric analysis. The findings of this study showed that increasing dietary Morus alba had positive effect on intestinal morphology parameters and in 1% it was significant (p<0.05). The duodenum villous length and width were significantly (P<0.05) greater in the group supplemented by 1% Morus alba than control. In addition, high levels of Morus alba leaves (0.5% and 1%) increased the surface of small intestine compared to the control group.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
14
v.
4
no.
2018
13
20
https://www.ivj.ir/article_81734_135c07d2b710b76b5b8c4087e64c962d.pdf
dx.doi.org/10.22055/ivj.2018.34650.1591
Macroscopic study of lymph nodes development in sheep fetus
Kaveh
Khazaeel
Assistant Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
Mahmood
Khaksary Mahabady
Associated Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
Mahdi
Pourmahdi
Associated Professor, Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
Behnam
Yazdanjoo
DVM Graduated from Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
text
article
2018
per
Due to the importance of immune system and important roles of lymph nodes against pathogens and with regard to there were not any study about anatomy of lymph nodes in sheep fetuses, this research was conducted to study macroscopic developmental growth of lymph nodes in fetal period. Also, anatomical specifications and estimating time of formation of some important lymph nodes evaluated. For this purpose, sixty sheep fetuses collected from slaughterhouse of Ahvaz, after fixation in 10% formalin solution and sex determination, were divided into to four groups according to CRL. Mandibular, superficial cervical (prescapular), caudal mediastinal, jejunal mesenteric and popliteal lymph nodes from five lymphocenters included head, neck, thorax, abdominal viscera and pelvic limb was evaluated, respectively. In each sample, appearance formation of nodes was studied and in the case of formation, gross shape, location, and their number were checked. Minimum and maximum of fetuses CRL were detected 3.5 and 48.5 cm(43 to 138 days old). In 32 male and 28 female studied fetuses, Sexuality had no effect on checked parameters. Unusual observations were not recorded in location of the nodes. Study of the lymph nodes in sheep fetuses showed that before 59 days old, all lymph nodes were not been visible macroscopically and before 45 days old, none of lymph nodes were macroscopic developed.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
14
v.
4
no.
2018
21
30
https://www.ivj.ir/article_81735_d64f60037bf7a5a1b9739c9a38841741.pdf
dx.doi.org/10.22055/ivj.2017.89720.1927
Evaluation of negative energy balance and some metabolic disorders on peri-partum period in Arabian ewes, Khuzestan
Amir
Zakian
Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
author
MohammadRahim
Haji-Hajikolaei
Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
Meysam
Tehrani-Sharif
Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Garmsar Branch, Garmsar, Iran
author
Kokab
Faramarziyan
DVSc of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
Pegah
Safaei-Borujeni
DVSc of Veterinary Clinical Pathology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
text
article
2018
per
BACKGROUND: According to a tradition of raising sheep and goats and economic dependence of rural households to produce meat and wool, therefore, one of the most important challenges in this livestock is establishing energy balance and nutritional needs. OBJECTIVES: In the present study to determine the prevalence of negative energy balance and to investigate some metabolic disorders around lambing conducted. METHODS: For this purpose 148 blood samples from pregnant (104) and non-pregnant (44) ewes collected and the age and the BCS also recorded. Serum concentrations of BHB, calcium, glucose, urea, total protein and total antioxidant capacity (TAC) was measured by routine laboratory methods. RESULTS: The results showed significant differences between pregnant and non-pregnant sheep from point of BHB (P<0.001), calcium (P<0.01), glucose (P<0.01), urea (P<0.001) concentrations and also between single and twin pregnant sheep from point of age (P<0.001), BHB (P<0.001), calcium (P<0.001), glucose (P<0.001), urea (P<0.001) and TAC (P<0.001) concentrations. Interaction of correlation between age and BCS with serum BHB concentration evaluated, there was no statistically significant correlation (P>0.05) but statistically significant negative correlation between the concentration of BHB and urea (P<0.05) and a significant positive correlation (P<0.05) between BHB and glucose was observed. It was also found that 16.35, 46.15 and 97.11 percent of pregnant ewes in this study suffering from hypoglycemia, hypocalcemia and subclinical pregnancy toxemia (hyperketonaemia), respectively, and accordingly peri-partum metabolic disorders in Arabic sheep was worse than imagined. CONCLUSIONS: It can be concluded, the overwhelming population of Khuzestan province's livestock are sheep; as a result, attention to the nutritional and management issues, especially in a terminal month of pregnancy and the first few weeks of birth emerge more than ever.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
14
v.
4
no.
2018
31
41
https://www.ivj.ir/article_81736_b17d50eb3ddeb95a2bbe0c94473cd64a.pdf
dx.doi.org/10.22055/ivj.2017.83433.1906
Molecular typing of avian metapneumovirus in broiler chickens in Isfahan province
Bahzad
Sepiani
DVM Graduated from Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
author
Majid
Gholami-Ahangaran
Associate Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
author
Hassan
Momtaz
Professor, Department of Microbiology, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
author
text
article
2018
per
Avian metapneumovirus is one of agents that it play role in inducing respiratory signs in birds. For study of common type of avian metapneumovirus (AMPV) in broiler chickens in Isfahan province, 35 broiler chicken flocks with high mortality were sampled. After RNA extraction and synthesis of cDNA, AMPV was investigated by one pair of specific primer. The AMPV positive samples were investigated for AMPV types (A, B, C and D) by type specific primers. Results showed 17 out of 35 (48.57%) of flocks were infected to AMPV. In this study, 67 out of 210 samples (31.90%) were positive for AMPV. The mean of morbidity to AMPV was 54.90% in sampled flocks. The typing of positive samples showed all of positive samples belonged to B type. In conclusion, by considering to high distribution of B type of AMPV in broiler chicken flocks, it is necessary to apply a proper AMPV vaccination program with autogenic strains, after approval of pathogenicity of AMPV strains.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
14
v.
4
no.
2018
42
49
https://www.ivj.ir/article_81737_cfbb572b32464009548529c5c177d457.pdf
dx.doi.org/10.22055/ivj.2017.65695.1826
Biological Evaluation of Different Di-calcium Phosphate Produced in Iran and Comparison of Their Impacts on Performance and Bone Characteristics in Male Broiler Chicks
Farzin
Ali malayeri
PhD Student of Animal and Poultry Health and Nutrition, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Mohsen
Farkhoy
Associate Professor, Department of Animal and Poultry Health and Nutrition, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Mehrdad
Modirsanei
Associate Professor, Department of Animal and Poultry Health and Nutrition, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Masood
Hashemzadeh
Research Assistant Professor, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension organization, Karaj, Iran
author
Mohammad
Rezaeen
Associate Professor, Department of Animal and Poultry Health and Nutrition, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
text
article
2018
per
Abstract In this study, in order to evaluate the produced domestic Di-calcium phosphate(DCP), 26 domestic DCP production factories that were active at the time of the research were visited and sampling was done randomly from the final product. After testing according to standard indices, only 7 samples of Di-calcium phosphate were in range of the national standard. To determine their biological value, 720 male Ross 308 broiler chickens were purchased and randomly divided into 8 equal groups with 6 replicates and then kept in the battery cage system for 21 days. The available phosphorus levels in all the diets used in the experimental and control groups were set at 0.48 percent. At the 10 and 21 days of age, feed consumption, weight gain and feed conversion ratio were calculated. Also At the end of 21 days of age the amount of ash, calcium and phosphorus of tibia bone was measured. At the 21 days of age, no significant difference was observed between experimental and control treatments in terms of the production indices (P>0.05) But the weight gain index at 10 days of age showed a significant difference between experimental and control treatments (P<0.05). There was no significant difference between bone ash content and test and control samples (P>0.05). But, the amounts of tibia calcium in two groups of F and G were significantly lower than those of the control group (P<0.05).Also the amounts of bone phosphorus in B, C, F and G groups were significant and lower than the control group (P<0.001). The relative biological values of the experimental samples, according to both criteria of the weight gain and the bone ash for the Di-calcium phosphates of A, B, C, D, E, F, and G samples were estimated 98.1, 103.3, 96.9, 100.2, 97.9, 94.0, and 100.2 percent, respectively.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
14
v.
4
no.
2018
50
60
https://www.ivj.ir/article_81738_361d4838fa837ec85a5625ac81db1c05.pdf
dx.doi.org/10.22055/ivj.2017.91829.1937
Evaluation of dry cow therapy effects on mamary infections and milk production rates in holstein breed
saad
Gouraninezhad
Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
Naghmeh
Moori Bakhtiari
Assistant Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
Moslem
Shahpari
DVM Graduated from Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
text
article
2018
per
The infection in dairy heifers may damage the developing secretary tissues reducing the future milk production in such animals. The efficacy of antibiotics for treating MI in unbred and primigravid heifers has been demonstrated by a few researchers. The objective of the present study was to compare the times of dry cow therapy in control of post partum MI and milk production in primigravid heifers. For this porpose, 75 primigravid heifers were selected and placed in three groups including: 60 day before parturition (group A), 30 day before parturition (group B), and 30-60 day before parturition (group c). Perepartum mammary glands secretion were collected to investigate microbial contamination.Then, intra mammary treatment with cloxacillin ointment was done in 50 heifers in two groups of A and B. In 3th day after parturition, 10 ml milk sample of 75 heifers was collected separately in 12ml sterile tube to investigate microbial contamination. Milk producing of heifers in three groups was recorded in 30, 60 and 90 day after parturition. Based on bacterial culture results mammary gland secretaries, in before and after parturition, the least bacterial contamination was observed in group B heifers and the most contamination was observed in control group of heifers. Between Heifers in group A and C, significant difference were found. By survey on milk production of three group heifers, it was found that there is significant differentiation between milk production in 30 and 90 day after parturition in three group and maximum production was demonstrated in group B and then in heifers group A. In this research, the best response to the treatment was demonstrated in group B, but, because of probability of antibiotic persistence in milk, recommended that dry cow therapy be done in 60 day before parturition.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
14
v.
4
no.
2018
61
70
https://www.ivj.ir/article_81740_26922ca30cb34cb1ae98bea8643147fd.pdf
dx.doi.org/10.22055/ivj.2017.87723.1917
The effect of oral administration of Carwey (Bunium persicum L) seed aqueous extract on abomasal emptying in neonatal lambs
Hamidreza
Mohammadi
Assistant Professor, Department of Clinical Science, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran
author
Mostafa
Abdollahi
DVM Students, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran
author
Ashkan
Jebelli-Javan
Associate Professor, Department of Food Quality Control and Hygiene, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran
author
text
article
2018
per
Abomasal hypomotility plays an important role in pathogenesis of some abomasal disorders such as abomasal bloat that there are same serious side effects associated with using synthetic drugs for its treatment, such as diarrhea and antibiotic resistance and for decreasing these side effects, administration of herbal medicine can be appropriate approach. Evaluating effect of Bunium persicum L on lamb,s abomasal emptying is goal of this study. This study was conducted on twelve five-day-olds Sangsari-female-lamb (average weight 4 kg). lambs received five oral treatments including salin (30ml), Eeythromycin (100 mg/kg), Carwey (0.4 gr/kg), Carwey (0.6 gr/kg) and Carwey (0.8 gr/kg), respectively. Acetaminophen absorption test was used to evaluating the rate of abomasal emptying. After drawing relational model between plasma Acetaminophen concentration and time with regression method showed that treating with erythromycin and different levels of aqueous extract of Carwey seed (0.4, 0.6 and 0.8 g/kg) increased the rate of abomasal emptying in comparison to the negative control, significantly (P<0.05). The stimulatory effect of erythromycin on abomasal emptying was higher than aquatic extract of Carwey seed, significantly (P<0.05). No clinical side effect were observed following the administration of erythromycin and Carwey in lambs. This study showed that aqueous extract of Carwey seed has a stimulatory effect on lamb's abomasal emptying but more studies are needed on the effect of this seed,s components on abomasal emptying.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
14
v.
4
no.
2018
70
76
https://www.ivj.ir/article_81743_776ec6dfba60eee467d6497d7b494f16.pdf
dx.doi.org/10.22055/ivj.2018.94409.1946
Histomorphometrical evaluation of Bursa of Fabricius and Immunohistochemistry Tracing of P53 and Caspase3 in
broiler following physiological stress and protective effect of chromium supplement
Reza
Moeinimoghadam
PhD Student of Histology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Hassan
Morovvati
Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Masoud
AdibMoradi
Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Seyed Davoud
Sharifi
Associate Professor, Department of Animal and Poultry Sciences, Abureyhan Campus, University of Tehran, Tehran, Iran
author
Ali
Shalizar Jalaly
Assistance Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
text
article
2018
per
The purpose of present study was to examine the protective effect of chromium supplement against damages which is induced by physiological stress and evaluation of apoptosis in Bursa of Fabricius of broilers through Immunohistochemical tracing of p53 and caspase-3 proteins. in this experimental study, 320 male Ross broilers were used. This study was designed as a 2 x 4 factorial, with two stress levels (under stress and stress free) and four levels of chromium-methionine supplement (0, 1000, 2000 and 3000ppb) in diet, which distributed into 4 accidental repeated groups of 10 each. stress was induced by adding dexamethasone to the diet, during 17 to 24 days of age. At the end, broilers in all groups were euthanized and the samples that includes Bursa of Fabricius were obtained then pathological and immunohistochemical parameters were evaluated. Physiological stress caused significant changes in medulla and cortex in lymphatic follicle of Bursa of Fabricus. Based on this study, Chromium-methionine supplementation could ameliorate the effects of Physiological stress in Bursa of Fabricius. Physiological stress caused significant increases in p53 and caspase-3 expression levels in Bursa of Fabricius (P<0/05). Chromium-methionine administration markedly reduced the expression levels of p53 and caspase-3 after inducing physiological stress (P<0/05).
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
14
v.
4
no.
2018
77
86
https://www.ivj.ir/article_81744_4256f5f459d869fd61e5dfdf76111a7f.pdf
dx.doi.org/10.22055/ivj.2018.99762.1967
Effects of glutamine protected on body condition score changes and plasma metabolites of Holstein fresh cows
Mostafa
Nemati
- Assistant Professor, Department of Microbiology, Faculty of Para Veterinary, Ilam University, Ilam, Iran
author
Sedigheh
Menatian
Assistant Professor, Department of Animal Science, Faculty of Para Veterinary, Ilam University, Ilam, Iran
author
Shahrzad
Joz ghasemi
PhD Graduated of Animal Nutrition, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
author
Reza
Hoshmandfar
Expert, Department of Microbiology, Faculty of Para Veterinary, Ilam University, Ilam, Iran
author
Mohsen
Taheri
MSc Graduated of Microbiology, Faculty of Para Veterinary, Ilam University, Ilam, Iran
author
Taieb
Saifi
Expert, Department of Microbiology, Faculty of Para Veterinary, Ilam University, Ilam, Iran
author
text
article
2018
per
This study was conducted to evaluate the effects of protected glutamine (Gln) supplementation in the diet of Holstein fresh cows after parturition on dry matter intake (DMI), plasma metabolites, body condition score (BCS) and reproductive performance. Forty Holstein dairy cows (796±58 kg of pre-parturition live weight; 3.25±0.35 BCS) at zero d of parturition were divided to four groups (n=10), including: basal diet (control group: a total mixed ration (TMR) consisting of 49% forage and 51% concentrate mixture on dry matter (DM) basis), basal diet supplemented with 150, 250 or 350 g of Gln protected with formaldehyde/cow per day. Dry matter intake of experimental treatments on 21 d after calving were 12.09, 14.39, 15.40 and 97.15 kg/d respectively (P=0.02). Plasma glucose concentrations of 1 to 4 treatments on 21 d after calving were 48.8, 55.0, 59.2 and 60.5 mg/dl respectively (P<0.01). total protein concentrations of 1 to 4 treatments on 21 d after calving were 5.02, 5.98, 7.10 and 7.20 g/dl respectively (P<0.01). AST concentrations of 1 to 4 treatments on 21 d after calving were 132.5, 82.1, 73.3 and 71.3 U/l respectively (P<0.01). Dietary supplementation with protected Gln had no effect on blood urinary nitrogen (P>0.05). The cows that received Gln changed the BCS less than the control treatment (P=0.03). Dietary supplementation of Gln had no effect on reproductive performance and the number of artificial insemination leading to pregnancy and also the interval between calving to pregnancy were not significant between treatments (P <0.05).
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
14
v.
4
no.
2018
87
95
https://www.ivj.ir/article_81745_cf0e8bb267c6f86342cf6066f5ff00a2.pdf
dx.doi.org/10.22055/ivj.2017.83194.1901
Immunogenicity of Infectious Coryza vaccine against a native isolate of Avibacterium paragallinarum from Iran
Abbas
Nouri
Research Assistant Professor, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
author
Mansour
Banani
Research Associate Professor, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
author
Reza
Toroqi
Research Associate Professor, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Mashhad, Iran
author
text
article
2018
per
Infectious Coryza (IC) an acute respiratory disease of chicken that caused by Avibacterium paragallinarum( Av.P). Immunity offered by a commercial IC killed oil vaccine against a native field isolate of Av. P serogroup A of Iran was studied by designing a vaccination / challenge experiment. 36 SPF birds of 14 weeks old were randomly divided into three separate groups. One group was vaccinated by two doses within 2 weeks by a commercial vaccine. After two weeks of last vaccination this group and the second one were challenged with 1×10 8 CFU/ml bacterial suspension prepared from fresh 24 hours cultured of Av. p through infra orbital sinus. The third group kept as control only received phosphate buffer saline. Three birds from each group were bled for serum sample collection on days of 2, 4, 6 and 8 after challenge. On Clinical observation of first group, mild swelling on inoculation site was developed on second day of experiment that gradually disappeared on days after. In second group on post infection day (PID) of 2, nasal discharge and facial swelling that extended to become bilateral beside tracheal rales were noticed in all bird throughout the experiments days. For all birds Sero-conversions monitored by serum plate agglutination (SPA) and agar gel precipitation (AGP) test using antigen prepared from 24 hours culture of bacterium. While birds of control remained negative, All bird of first group shown positive serum reaction by SPA and AGP tests while in second group, only SPA becomes positive after 6 PID. In conclusion, this native isolate have an antigenic relationship with vaccine contained strains and the commercial vaccine can prevent must clinical sign in infected birds. However, more studies needed to verify serovar identity of isolate and reach a conclusive outcomes about efficacy of currently used vaccine in country.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
14
v.
4
no.
2018
96
105
https://www.ivj.ir/article_81746_924ab7b595ec3b52aa5917f5ddd3ef5f.pdf
dx.doi.org/10.22055/ivj.2017.60335.1883
Frequency of iucD, tsh, and iss genes among Escherichia coli isolates in broilers infected with colibacillosis
Hossien
Nikpiran
Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University Tabriz, Branch, Tabriz, Iran
author
Saeed Mostafa
Peighambari
Professor, Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Akbar
Abdi Khasavan
Private Veterinarian, Zanjan, Iran
author
text
article
2018
per
Escherichia coli (E. coli) is component of normal flora of the human intestine, mammals and birds, however, some strains of E. coli due to have virulence factors, are pathogenic. The aim of current study was to determine the frequency of iucD, tsh, and iss genes in broiler flocks infected with colibacillosis in east Azarbaijan-Iran. One Hundred and seventeen E. coli isolates from colibacillosis infected broilers with pericarditis, perihepatitis, and air sacculitis investigated. First, standard screening tests and culture was done, and then standard primers and PCR was used for genotype evaluation of isolates in regard of iucD, tsh, and iss genes. In 29 samples (24.78%) all genes (iucD, iss, and tsh) was positive, and 29 (24.78%), 39 (33.33%), and 44 (37.60%) of samples was positive in regard of dual combinations of genes tsh/iss, tsh/iucD, and iss/iucD, respectively. In addition, 34.18%, 37.60%, and 91.45% of isolates possess tsh, iss, and iucD, respectively. Our results indicated which higher percent of evaluated isolates have the ability to produce aerobactin, and the various combination of genes seen in 24.785 to 37.60% of isolates. It seems more investigation needed to evaluation of virulence factors of east Azarbaijan broilers.
Iranian Veterinary Journal
Shahid Chamran University of Ahvaz
1735-6873
14
v.
4
no.
2018
106
114
https://www.ivj.ir/article_81747_e200a7ad7c1e572ed6e21c148e6c3b39.pdf
dx.doi.org/10.22055/ivj.2017.57760.1771